التفاصيل البيبلوغرافية
| العنوان: |
Detection of isoniazid and rifampin resistance of Mycobacterium tuberculosis by a multiplex allele-specific polymerase chain reaction (PCR) assay |
| المؤلفون: |
Allegui, Zeyneb, Ghariani, Asma, Draoui, Henda, Ghezal, Ameni, Mehiri, Emna, Slim-Saidi, Leila |
| المصدر: |
International Journal of Mycobacteriology; Mar2012, Vol. 1 Issue 1, p34-39, 6p |
| مصطلحات موضوعية: |
RIFAMPIN, ISONIAZID, MYCOBACTERIUM tuberculosis, POLYMERASE chain reaction, MULTIDRUG-resistant tuberculosis, DRUG resistance |
| مستخلص: |
Abstract: Background: The use of molecular techniques is a major improvement for the rapid routine detection and control of multidrug-resistant tuberculosis (MDR-TB). Materials: In this study, the multiplex allele-specific polymerase chain reaction (MAS-PCR) was developed to simultaneously detect the most frequent mutations associated with isoniazid (INH) and rifampin (RIF) resistance in a single assay. Results: The assay was tested with 53 clinical isolates. Among them, 27 were MDR strains, 17 were mono-resistant to INH, one was mono-resistant to RIF, and eight were susceptible. The MAS-PCR assay showed a specificity of 100% in detecting drug resistance. An equivalent sensitivity of 92.6% in detecting MDR and RIF-resistance was found. The sensitivity for the detection of INH-resistance was 88.6%. Conclusions: The MAS-PCR assay was a simple and rapid method for detecting the INH and RIF-resistance in Mycobacterium tuberculosis (MT) clinical strains. It is also easy to perform and to interpret. The assay is inexpensive and a less-demanding technique. [Copyright &y& Elsevier] |
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| قاعدة البيانات: |
Complementary Index |
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