التفاصيل البيبلوغرافية
| العنوان: |
Role of human oocyte-enriched factors in somatic cell reprograming. |
| المؤلفون: |
El-Gammal, Zaynab1, AlOkda, Abdelrahman1, El-Badri, Nagwa1 nelbadri@zewailcity.edu.eg |
| المصدر: |
Mechanisms of Ageing & Development. Oct2018, Vol. 175, p88-99. 12p. |
| مصطلحات موضوعية: |
SOMATIC cells, OVUM physiology, MOLECULAR chaperones, CHROMATIN-remodeling complexes, TRANSCRIPTION factors, RNA-binding proteins |
| مستخلص: |
Highlights • Oocyte enriched factors are able to reprogram somatic cell nuclei towards an embryonic stem cell-like state. • Reprograming effect of oocyte-specific factors is achieved through metabolic switch, chromatin remodeling, and global epigenetic transformations. • These factors include chromatin remodeling enzymes, histone remodelling chaperones, pluripotency associated factors, transcription factors, chromatin remodeling subunits, regulators of mitochondrial homeostasis, Akt activator, RNA-binding proteins, along with other factors. • Characterization of these factors is critical for improving the quality of resulting reprogramed cells, which is crucial for therapeutic applications. Abstract Cellular reprograming paves the way for creating functional patient-specific tissues to eliminate immune rejection responses by applying the same genetic profile. However, the epigenetic memory of a cell remains a challenge facing the current reprograming methods and does not allow transcription factors to bind properly. Because somatic cells can be reprogramed by transferring their nuclear contents into oocytes, introducing specific oocyte factors into differentiated cells is considered a promising approach for mimicking the reprograming process that occurs during fertilization. Mammalian metaphase II oocyte possesses a superior capacity to epigenetically reprogram somatic cell nuclei towards an embryonic stem cell-like state than the current factor-based reprograming approaches. This may be due to the presence of specific factors that are lacking in the current factor-based reprograming approaches. In this review, we focus on studies identifying human oocyte-enriched factors aiming to understand the molecular mechanisms mediating cellular reprograming. We describe the role of oocyte-enriched factors in metabolic switch, chromatin remodelling, and global epigenetic transformation. This is critical for improving the quality of resulting reprogramed cells, which is crucial for therapeutic applications. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
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