المؤلفون: Bombelli, Alberto, Araya-Cloutier, Carla, Boeren, Sjef, Vincken, Jean Paul, Abee, Tjakko, den Besten, Heidy M.W.

المصدر: Food Research International 175 (2024) ; ISSN: 0963-9969

مصطلحات موضوعية: Cell envelope, Cross-protection, LiaSR, Mode of action, Natural antimicrobials, Prenylated isoflavonoids, SigmaB regulon, Two-component system, VirRS

الوصف: Glabridin is a prenylated isoflavan which can be extracted from liquorice roots and has shown antimicrobial activity against foodborne pathogens and spoilage microorganisms. However, its application may be hindered due to limited information about its mode of action. In this study, we aimed to investigate the mode of action of glabridin using a combined phenotypic and proteomic approach on Listeria monocytogenes. Fluorescence and transmission electron microscopy of cells exposed to glabridin showed membrane permeabilization upon treatment with lethal concentrations of glabridin. Comparative proteomics analysis of control cells and cells exposed to sub-lethal concentrations of glabridin showed upregulation of proteins related to the two-component systems LiaSR and VirRS, confirming cell envelope damage during glabridin treatment. Additional upregulation of SigmaB regulon members signified activation of the general stress response in L. monocytogenes during this treatment. In line with the observed upregulation of cell envelope and general stress response proteins, sub-lethal treatment of glabridin induced (cross)protection against lethal heat and low pH stress and against antimicrobials such as nisin and glabridin itself. Overall, this study sheds light on the mode of action of glabridin and activation of the main stress responses to this antimicrobial isoflavan and highlights possible implications of its use as a naturally derived antimicrobial compound.

وصف الملف: application/pdf

العلاقة: https://edepot.wur.nl/645829Test; https://research.wur.nl/en/publications/effects-of-the-antimicrobial-glabridin-on-membrane-integrity-andTest-

الإتاحة: https://doi.org/10.1016/j.foodres.2023.113687Test
https://research.wur.nl/en/publications/effects-of-the-antimicrobial-glabridin-on-membrane-integrity-andTest-

المؤلفون: Cloeckaert, Axel, Roop, R. Martin, Scholz, Holger, Whatmore, Adrian, Zygmunt, Michel S

المساهمون: Infectiologie et Santé Publique (UMR ISP), Université de Tours (UT)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), East Carolina University Greenville (ECU), University of North Carolina System (UNC), Centre for Biological Threats and Special Pathogens Berlin (ZBS), Robert Koch Institute Berlin (RKI), Animal and Plant Health Agency Addlestone, UK (APHA)

المصدر: ISSN: 1664-302X.

مصطلحات موضوعية: Brucellaceae, Brucella, Ochrobactrum, genetics/genomics, diversity, evolution, cell envelope, virulence, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology

الوصف: International audience

العلاقة: info:eu-repo/semantics/altIdentifier/pmid/38333583; hal-04523408; https://hal.inrae.fr/hal-04523408Test; https://hal.inrae.fr/hal-04523408/documentTest; https://hal.inrae.fr/hal-04523408/file/2024_Clockaert_Front-Microbiol_vol-15.pdfTest; PUBMED: 38333583; PUBMEDCENTRAL: PMC10850559; WOS: 001158508700001

الإتاحة: https://doi.org/10.3389/fmicb.2024.1370330Test
https://doi.org/10.3389/fmicb.2024.1370330/fullTest
https://hal.inrae.fr/hal-04523408Test
https://hal.inrae.fr/hal-04523408/documentTest
https://hal.inrae.fr/hal-04523408/file/2024_Clockaert_Front-Microbiol_vol-15.pdfTest

المؤلفون: Abhishek Mondal, Manisha Siwach, Manzoor Ahmad, Sunish Kumar Radhakrishnan, Pinaki Talukdar

مصطلحات موضوعية: Biophysics, Biochemistry, Pharmacology, Biotechnology, Evolutionary Biology, Developmental Biology, Infectious Diseases, Biological Sciences not elsewhere classified, Chemical Sciences not elsewhere classified, Information Systems not elsewhere classified, sup >–, potent antibacterial agents, negative bacterial strains, linked hetero hydrazones, evolving challenges posed, efficient antibacterial activity, cell envelope homeostasis, become increasingly difficult, transmembrane h, therefore imperative, specific class, promising avenue, ongoing pursuit, high efficacy, hcl transport, different gram, develop resistance, cooperative protonation, compounds offer, compounds inhibits

الوصف: The development of potent antibacterial agents has become increasingly difficult as bacteria continue to evolve and develop resistance to antibiotics. It is therefore imperative to find effective antimicrobial agents that can address the evolving challenges posed by infectious diseases and antimicrobial resistance. Using artificial transmembrane ion transporters is an emerging and promising avenue to address this issue. We report pyridyl-linked hetero hydrazones as highly efficient transmembrane HCl symporters. These compounds offer an appropriate HCl binding site through cooperative protonation, followed by recognition of chloride ions. HCl transport by these compounds inhibits the growth of different Gram-negative bacterial strains with high efficacy by affecting the cell envelope homeostasis. This specific class of compounds holds substantial promise in the ongoing pursuit of developing highly efficient antibacterial agents.

العلاقة: https://figshare.com/articles/journal_contribution/Pyridyl-Linked_Hetero_Hydrazones_Transmembrane_H_sup_sup_Cl_sup_sup_Symporters_with_Efficient_Antibacterial_Activity/25050614Test

الإتاحة: https://doi.org/10.1021/acsinfecdis.3c00455.s001Test
https://figshare.com/articles/journal_contribution/Pyridyl-Linked_Hetero_Hydrazones_Transmembrane_H_sup_sup_Cl_sup_sup_Symporters_with_Efficient_Antibacterial_Activity/25050614Test

المؤلفون: Raveschot, Cyril, Cudennec, Benoit, Deracinois, Barbara, Frémont, Marc, Vaeremans, Maxime, Dugersuren, Jamyan, Demberel, Shirchin, Drider, Djamel, Dhulster, Pascal, Coutte, Francois, Flahaut, Christophe

المساهمون: INRA, ISA, Univ. Artois, Univ. Littoral Côte d’Opale, Université de Lille, 1057473|||BioEcoAgro - UMR transfrontalière INRAE 1158 (VALID), Institut Charles Viollette (ICV) - ULR 7394 ICV, BioEcoAgro - UMR-T 1158, BioEcoAgro - UMR transfrontalière INRAE 1158, Institut Charles Viollette (ICV) - ULR 7394

مصطلحات موضوعية: Cell envelope associated peptidases, fermented milk, Lactobacillus, peptides

الوصف: The aim of our study was to characterize the proteolytic activity of 170 Lactobacillus strains isolated from traditional Mongolian dairy products (yogurt and fermented milk), and to investigate their capacity to generate bioactive peptides during milk fermentation. All isolates were screened for proteolytic activity using skim milk agar-well diffusion test. Fifteen strains (9 Lactobacillus helveticus and 6 Lactobacillus delbrueckii subsp. bulgaricus) were then selected and further evaluated using an original strategy based on multiparametric analysis, taking into account growth rate, acidification capacity, proteolytic activity, cell envelope associated peptidase (CEP) profile and LC–MS/MS analysis of peptides. All parameters were analyzed using principal component analysis (PCA). Results showed that strain growth and acidification correlate with peptide production and that Mongolian L. helveticus strains differ from Western strains in terms of CEP distribution. The PCA revealed that CEP profiles are major determinants of β-casein hydrolysis patterns. Strains with distinctive proteolytic activities were identified. ; 304

وصف الملف: application/octet-stream

العلاقة: Plateforme intégREe AppLiquée au criblage haut débit de CATalyseurs pour les bioraffineries; Food Chemistry; http://hdl.handle.net/20.500.12210/58318Test

الإتاحة: https://doi.org/20.500.12210/58318Test
https://hdl.handle.net/20.500.12210/58318Test

المؤلفون: Cloeckaert, Axel, Roop, R. Martin, Scholz, Holger, Whatmore, Adrian, Zygmunt, Michel S

المساهمون: Infectiologie et Santé Publique (UMR ISP), Université de Tours (UT)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), East Carolina University Greenville (ECU), University of North Carolina System (UNC), Centre for Biological Threats and Special Pathogens Berlin (ZBS), Robert Koch Institute Berlin (RKI), Animal and Plant Health Agency Addlestone, UK (APHA)

المصدر: ISSN: 1664-302X.

مصطلحات موضوعية: Brucellaceae, Brucella, Ochrobactrum, genetics/genomics, diversity, evolution, cell envelope, virulence, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology

الوصف: International audience

العلاقة: info:eu-repo/semantics/altIdentifier/pmid/38333583; hal-04523408; https://hal.inrae.fr/hal-04523408Test; https://hal.inrae.fr/hal-04523408/documentTest; https://hal.inrae.fr/hal-04523408/file/2024_Clockaert_Front-Microbiol_vol-15.pdfTest; PUBMED: 38333583; PUBMEDCENTRAL: PMC10850559; WOS: 001158508700001

الإتاحة: https://doi.org/10.3389/fmicb.2024.1370330Test
https://doi.org/10.3389/fmicb.2024.1370330/fullTest
https://hal.inrae.fr/hal-04523408Test
https://hal.inrae.fr/hal-04523408/documentTest
https://hal.inrae.fr/hal-04523408/file/2024_Clockaert_Front-Microbiol_vol-15.pdfTest

المؤلفون: Moustafa A. TagElDein, Noha G. Mohamed, Yasser E. Shahein, Laila Ziko, Nahla A. Hussein

مصطلحات موضوعية: Bacterial Physiology and Genetics, Genetics, FOS Biological sciences, Biochemistry, Genetics and Molecular Biology, Life Sciences, Ribosome Structure and Translation Mechanisms, Molecular Biology, Cyclotide Bioengineering and Protein Anchoring Mechanisms, Membrane Proteins, Bacterial outer membrane, Cell envelope, Cell biology, Biology, Escherichia coli, Porin, Signal transduction, Biochemistry, Gene

الوصف: Escherichia coli cell envelope is crucial for stress sensing and signal transduction, mediated by numerous protein–protein interactions to enable adaptation and survival. Interfering with these interactions might affect envelope integrity leading to bacterial death. The outer membrane lipoprotein (RcsF) is the stress sensor of the regulator of capsule synthesis (Rcs) phosphorelay that senses envelope threats. RcsF interacts with two essential proteins, IgaA (repressing the Rcs system) and BamA (inserting β-barrel proteins in the outer membrane). Disturbing RcsF interactions may alter Rcs signaling and/or membrane integrity thus affecting bacterial survival. Here, we derived the sequence of a peptide mimicking RcsF (RcsFmim), based on the in silico docking of RcsF with IgaA. Expression of rcsF mim caused 3-to-4-fold activation of the Rcs system and perturbation of the outer membrane. Both effects result in decreased E. coli growth rate. We anticipate that RcsFmim present a candidate for future ... : يعد غلاف خلية الإشريكية القولونية التجريدية أمرًا بالغ الأهمية لاستشعار الإجهاد ونقل الإشارة، بوساطة العديد من تفاعلات البروتين والبروتين لتمكين التكيف والبقاء على قيد الحياة. قد يؤثر التدخل في هذه التفاعلات على سلامة المغلف مما يؤدي إلى موت البكتيريا. البروتين الدهني الغشائي الخارجي (RcsF) هو مستشعر الإجهاد لمنظم التركيب الفوسفوري للكبسولة (Rcs) الذي يستشعر تهديدات الغلاف. يتفاعل RcsF مع اثنين من البروتينات الأساسية، IgaA (قمع نظام Rcs) و BamA (إدخال بروتينات β - barrel في الغشاء الخارجي). قد تؤدي تفاعلات RcsF المزعجة إلى تغيير إشارات Rcs و/أو سلامة الغشاء مما يؤثر على بقاء البكتيريا. هنا، اشتقنا تسلسل محاكاة الببتيد RcsF (RcsFmim)، بناءً على الالتحام في السيليكو لـ RcsF مع IgaA. تسبب تعبير rcsF mim في تنشيط نظام Rcs من 3 إلى 4 أضعاف واضطراب الغشاء الخارجي. يؤدي كلا التأثيرين إلى انخفاض معدل نمو الإشريكية القولونية. نتوقع أن تقدم RcsFmim مرشحًا لتطوير الببتيد المضاد للبكتيريا في المستقبل. ...

العلاقة: https://dx.doi.org/10.60692/9xtf7-56g40Test

الإتاحة: https://doi.org/10.60692/m8h9v-y0j3910.60692/9xtf7-56g40Test

المؤلفون: Moustafa A. TagElDein, Noha G. Mohamed, Yasser E. Shahein, Laila Ziko, Nahla A. Hussein

مصطلحات موضوعية: Bacterial Physiology and Genetics, Genetics, FOS Biological sciences, Biochemistry, Genetics and Molecular Biology, Life Sciences, Ribosome Structure and Translation Mechanisms, Molecular Biology, Cyclotide Bioengineering and Protein Anchoring Mechanisms, Membrane Proteins, Bacterial outer membrane, Cell envelope, Cell biology, Biology, Escherichia coli, Porin, Signal transduction, Biochemistry, Gene

الوصف: Escherichia coli cell envelope is crucial for stress sensing and signal transduction, mediated by numerous protein–protein interactions to enable adaptation and survival. Interfering with these interactions might affect envelope integrity leading to bacterial death. The outer membrane lipoprotein (RcsF) is the stress sensor of the regulator of capsule synthesis (Rcs) phosphorelay that senses envelope threats. RcsF interacts with two essential proteins, IgaA (repressing the Rcs system) and BamA (inserting β-barrel proteins in the outer membrane). Disturbing RcsF interactions may alter Rcs signaling and/or membrane integrity thus affecting bacterial survival. Here, we derived the sequence of a peptide mimicking RcsF (RcsFmim), based on the in silico docking of RcsF with IgaA. Expression of rcsF mim caused 3-to-4-fold activation of the Rcs system and perturbation of the outer membrane. Both effects result in decreased E. coli growth rate. We anticipate that RcsFmim present a candidate for future ... : يعد غلاف خلية الإشريكية القولونية التجريدية أمرًا بالغ الأهمية لاستشعار الإجهاد ونقل الإشارة، بوساطة العديد من تفاعلات البروتين والبروتين لتمكين التكيف والبقاء على قيد الحياة. قد يؤثر التدخل في هذه التفاعلات على سلامة المغلف مما يؤدي إلى موت البكتيريا. البروتين الدهني الغشائي الخارجي (RcsF) هو مستشعر الإجهاد لمنظم التركيب الفوسفوري للكبسولة (Rcs) الذي يستشعر تهديدات الغلاف. يتفاعل RcsF مع اثنين من البروتينات الأساسية، IgaA (قمع نظام Rcs) و BamA (إدخال بروتينات β - barrel في الغشاء الخارجي). قد تؤدي تفاعلات RcsF المزعجة إلى تغيير إشارات Rcs و/أو سلامة الغشاء مما يؤثر على بقاء البكتيريا. هنا، اشتقنا تسلسل محاكاة الببتيد RcsF (RcsFmim)، بناءً على الالتحام في السيليكو لـ RcsF مع IgaA. تسبب تعبير rcsF mim في تنشيط نظام Rcs من 3 إلى 4 أضعاف واضطراب الغشاء الخارجي. يؤدي كلا التأثيرين إلى انخفاض معدل نمو الإشريكية القولونية. نتوقع أن تقدم RcsFmim مرشحًا لتطوير الببتيد المضاد للبكتيريا في المستقبل. ...

العلاقة: https://dx.doi.org/10.60692/m8h9v-y0j39Test

الإتاحة: https://doi.org/10.60692/9xtf7-56g4010.60692/m8h9v-y0j39Test

المؤلفون: Segers, Anneleen, de Vos, Willem M.

المصدر: Microbiome Research Reports 2 (2023) 1 ; ISSN: 2771-5965

مصطلحات موضوعية: Akkermansia muciniphila, Amuc_1100, P9, cell envelope components, extracellular and secreted proteins, metabolites, pilus-associated signaling protein

الوصف: Akkermansia muciniphila is a promising next-generation beneficial microbe due to its natural presence in the mucus layer of the gut, its symbiotic ability to degrade mucus, and its capacity to improve the intestinal barrier function. A. muciniphila is able to counteract weight gain and immuno-metabolic disturbances in several animal models. Many of these disorders, including obesity and auto-immune diseases, have been associated with decreased gut barrier function and consequent increased inflammation. Since A. muciniphila was found to normalize these changes and strengthen the gut barrier function, it is hypothesized that other beneficial effects of A. muciniphila might be caused by this restoration. In search for A. muciniphila’s mode of action in enhancing the gut barrier function and promoting health, we reasoned that secreted components or cell envelope components of A. muciniphila are interesting candidates as they can potentially reach and interact with the epithelial barrier. In this review, we focus on the potential mechanisms through which A. muciniphila can exert its beneficial effects on the host by the production of extracellular and secreted proteins, metabolites and cell envelope components. These products have been studied in isolation for their structure, signaling capacity, and in some cases, also for their effects in preclinical models. This includes the protein known as Amuc_1100, which we here rename as pilus-associated signaling (PAS) protein, the P9 protein encoded by Amuc_1631, the short-chain fatty acids acetate and propionate, and cell envelope components, such as phosphatidylethanolamine and peptidoglycan.

وصف الملف: application/pdf

العلاقة: https://edepot.wur.nl/658595Test; https://research.wur.nl/en/publications/mode-of-action-of-akkermansia-muciniphila-in-the-intestinal-dialoTest

الإتاحة: https://doi.org/10.20517/mrr.2023.05Test
https://research.wur.nl/en/publications/mode-of-action-of-akkermansia-muciniphila-in-the-intestinal-dialoTest

المؤلفون: Maher, Claire, Hassan, Karl A.

المصدر: Maher , C & Hassan , K A 2023 , ' The Gram-negative permeability barrier : tipping the balance of the in and the out ' , mBio , vol. 14 , no. 6 , pp. 1-15 . https://doi.org/10.1128/mbio.01205-23Test

مصطلحات موضوعية: antibiotic resistance, cell envelope, Gram-negative bacteria

الوصف: Gram-negative bacteria are intrinsically resistant to many antibiotics, due in large part to the permeability barrier formed by their cell envelope. The complex and synergistic interplay of the two Gram-negative membranes and active efflux prevents the accumulation of a diverse range of compounds that are effective against Gram-positive bacteria. A lack of detailed information on how components of the cell envelope contribute to this has been identified as a key barrier to the rational development of new antibiotics with efficacy against Gram-negative species. This review describes the current understanding of the role of the different components of the Gram-negative cell envelope in preventing compound accumulation and the state of efforts to describe properties that allow compounds to overcome this barrier and apply them to the development of new broad-spectrum antibiotics.

وصف الملف: application/pdf

الإتاحة: https://doi.org/10.1128/mbio.01205-23Test
https://researchers.mq.edu.au/en/publications/13aa5c55-036b-4766-ad8a-1f05b94850daTest
https://research-management.mq.edu.au/ws/files/343483171/343041061.pdfTest
http://www.scopus.com/inward/record.url?scp=85182913463&partnerID=8YFLogxKTest
http://purl.org/au-research/grants/arc/FT180100123Test

المؤلفون: Falchi, Federica A, Taylor, Rebecca J, Rowe, Sebastian J, Moura, Elisabete C C M, Baeta, Tiago, Laguri, Cedric, Simorre, Jean-Pierre, Kahne, Daniel E, Polissi, Alessandra, Sperandeo, Paola

المساهمون: F.A. Falchi, R.J. Taylor, S.J. Rowe, E.C.C.M. Moura, T. Baeta, C. Laguri, J. Simorre, D.E. Kahne, A. Polissi, P. Sperandeo

مصطلحات موضوعية: ABC transporter, ATPase, cell envelope, lipopolysaccharide transport, outer membrane biogenesi, proteoliposomes, Settore BIO/19 - Microbiologia Generale

الوصف: The presence of an external LPS layer in the outer membrane makes Gram-negative bacteria intrinsically resistant to many antibiotics. Millions of LPS molecules are transported to the cell surface per generation by the Lpt molecular machine made, in E. coli, by seven essential proteins.Lipopolysaccharide (LPS) is an essential component of the outer membrane (OM) of many Gram-negative bacteria, providing a barrier against the entry of toxic molecules. In Escherichia coli, LPS is exported to the cell surface by seven essential proteins (LptA-G) that form a transenvelope complex. At the inner membrane, the ATP-binding cassette (ABC) transporter LptB(2)FG associates with LptC to power LPS extraction from the membrane and transfer to the periplasmic LptA protein, which is in complex with the OM translocon LptDE. LptC interacts both with LptB(2)FG and LptADE to mediate the formation of the transenvelope bridge and regulates the ATPase activity of LptB(2)FG. A genetic screen has previously identified suppressor mutants at a residue (R212) of LptF that are viable in the absence of LptC. Here, we present in vivo evidence that the LptF R212G mutant assembles a six-protein transenvelope complex in which LptA mediates interactions with LptF and LptD in the absence of LptC. Furthermore, we present in vitro evidence that the mutant LptB(2)FG complexes restore the regulation of ATP hydrolysis as it occurs in the LptB(2)FGC complex to achieve wild-type efficient coupling of ATP hydrolysis and LPS movement. We also show the suppressor mutations restore the wild-type levels of LPS transport both in vivo and in vitro, but remarkably, without restoring the affinity of the inner membrane complex for LptA. Based on the sensitivity of lptF suppressor mutants to selected stress conditions relative to wild-type cells, we show that there are additional regulatory functions of LptF and LptC that had not been identified.IMPORTANCE The presence of an external LPS layer in the outer membrane makes Gram-negative bacteria intrinsically ...

العلاقة: info:eu-repo/semantics/altIdentifier/pmid/36541759; info:eu-repo/semantics/altIdentifier/wos/WOS:000900986900002; volume:14; issue:1; firstpage:1; lastpage:16; numberofpages:16; journal:MBIO; https://hdl.handle.net/2434/953186Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85149154477

الإتاحة: https://doi.org/10.1128/mbio.02202-22Test
https://hdl.handle.net/2434/953186Test