المؤلفون: Zhang, Yin, Zeng, Qing, Zhang, Yingjie, Zhang, Pengcheng, Li, Qing, Zhou, Jiao, Dong, Li, Pan, Zhongli

المصدر: Foods, vol 13, iss 5

مصطلحات موضوعية: 5′−nucleotidase, isolation and purification, pork, umami

الوصف: In this study, we established a new methodology for preparing 5-nucleotidase (5-NT) with the aim of enhancing our understanding of its enzyme activity and laying a basis for regulating the content of umami-enhancing nucleotides in pork. 5-NT was prepared with Sephadex gel filtration and reverse-phase high-performance liquid chromatography, and its enzymatic properties and catalytic activity were evaluated. The results show that the molecular weight of the prepared 5-NT was 57 kDa, the optimal catalytic temperature was 40 °C, and the optimal pH was 8. Zn2+, and sucrose showed inhibitory effects on the activity of 5-NT, while K+, Na+, Ca2+, Mg2+, glucose, fructose, and trehalose promoted the activity of the studied compound. The prepared 5-NT exhibited higher catalytic activity and selectivity against IMP compared with its commercial counterpart, while its catalytic activity against XMP was not significant (p > 0.05). In brief, we established a new methodology for preparing 5-NT, enhancing our understanding of its enzyme activity and providing a solid basis for regulating the content of umami-enhancing nucleotides in pork through the control of endogenous 5-NT activity.

وصف الملف: application/pdf

العلاقة: qt93m8s3wc; https://escholarship.org/uc/item/93m8s3wcTest

الإتاحة: https://escholarship.org/uc/item/93m8s3wcTest

المؤلفون: Wei, Rong Qiang, Zeng, Qing Li

مصطلحات موضوعية: Physics - Geophysics

الوصف: It is necessary to study the kinematics of landslide prior to its failure for accurately estimating the time of landslide instability. Based on a spring block model, considering the Dieterich Ruina's friction, the kinematic displacement and velocity of landslide along the slip surface are analyzed under quasistatic approximation. A algebraic relationship including three parameters between the displacement (or velocity) and time is obtained, and then applied to two typical landslides: Vaiont in Italy, and Maoxian in China. The results show that the proposed spring block friction model can well describe the kinematic data of landslides before their failure. If the effective data of displacement can be obtained to determine the three parameters above, this simple physical model could be used to estimate the time of landslide instability. This spring block friction model also provides clear physical basis for the usual inverse velocity method of the landslide warning, the stick slip of some landslides, and the scaling relationship between the numbers of the landslides and their volume. ; Comment: 16 pages; 7 figures; 1 Table

العلاقة: http://arxiv.org/abs/2401.09707Test

الإتاحة: http://arxiv.org/abs/2401.09707Test

المؤلفون: Zhang, Lijie, Yao, Qiuru, Hu, Jinjing, Qiu, Baizhi, Xiao, Yupeng, Zhang, Qi, Zeng, Yuting, Zheng, Shuqi, Zhang, Youao, Wan, Yantong, Zheng, Xiaoyan, Zeng, Qing

المساهمون: National Natural Science Foundation of China, Natural Science Foundation for Guangdong Province, Guangdong Province health appropriate technology promotion project, China Disabled Persons’ Federation Hearing and Language Disability Prevention and Rehabilitation Special, College Students’ Innovative Training Plan Program of China, College Students’ Innovative Training Plan Program of Guangdong Province

المصدر: European Journal of Medical Research ; volume 29, issue 1 ; ISSN 2047-783X

مصطلحات موضوعية: General Medicine

الوصف: Background Alzheimer's disease is one common type of dementia. Numerous studies have suggested a correlation between Alzheimer's disease and inflammation. Microglia mainly participate in the inflammatory response in the brain. Currently, ample evidence has shown that microglia are closely related to the occurrence and development of Alzheimer's disease. Objective We opted for bibliometric analysis to comprehensively summarize the advancements in the study of microglia in Alzheimer's disease, aiming to provide researchers with current trends and future research directions. Methods All articles and reviews pertaining to microglia in Alzheimer's disease from 2000 to 2022 were downloaded through Web of Science Core Collection. The results were subjected to bibliometric analysis using VOSviewer 1.6.18 and CiteSpace 6.1 R2. Results Overall, 7449 publications were included. The number of publications was increasing yearly. The United States has published the most publications. Harvard Medical School has published the most papers of all institutions. Journal of Alzheimer’s Disease and Journal o f Neuroscience were the journals with the most studies and the most commonly cited, respectively. Mt Heneka is the author with the highest productivity and co-citation. After analysis, the most common keywords are neuroinflammation, amyloid-beta, inflammation, neurodegeneration. Gut microbiota, extracellular vesicle, dysfunction and meta-analysis are the hotspots of research at the present stage and are likely to continue. Conclusion NLRP3 inflammasome, TREM2, gut microbiota, mitochondrial dysfunction, exosomes are research hotspots. The relationship between microglia-mediated neuroinflammation and Alzheimer's disease have been the focus of current research and the development trend of future research.

الإتاحة: https://doi.org/10.1186/s40001-023-01602-9Test

المؤلفون: Liu, Lu-lin, Liu, Zhong-rui, Cao, Lu-jun, Wang, Jun, Huang, San-ming, Hu, Shui-gen, Yang, Yi-zhong, Li, Dong-sheng, Cao, Wei-wei, Zeng, Qing-bao, Huang, Sheng, Wu, Qiong, Xiao, Jian-hua, Liu, Wu-yang, Xiao, Yao-sheng

المصدر: Journal of Orthopaedic Surgery and Research ; volume 19, issue 1 ; ISSN 1749-799X

مصطلحات موضوعية: Orthopedics and Sports Medicine, Surgery

الوصف: Objective Iron accumulation is associated with osteoporosis. This study aims to explore the effect of chronic iron accumulation induced by hepcidin1 deficiency on aging osteoporosis. Methods Iron accumulation in hepcidin1 knockout aging mice was assessed by atomic absorption spectroscopy and Perl’s staining. Bone microarchitecture was observed using Micro-CT. Hepcidin, ferritin, oxidative stress, and markers of bone turnover in serum were detected by enzyme-linked immunosorbent assay. Bone formation and resorption markers were measured by real-time quantitative PCR. Cell aging was induced by D-galactose treatment. CCK-8, flow cytometry, EdU assays, and Alizarin red staining were performed to reveal the role of hepcidin1 knockout in cell model. Iron Colorimetric Assay Kit and western blot were applied to detect iron and ferritin levels in cells, respectively. Results In hepcidin1-knockout mice, the ferritin and iron contents in liver and tibia were significantly increased . Iron accumulation induced by hepcidin1 knockout caused a phenotype of low bone mass and deteriorated bone microarchitecture. Osteogenic marker was decreased and osteoclast marker was increased in mice, accompanied by increased oxidative stress level. The mRNA expression levels of osteoclast differentiation markers (RANKL, Mmp9, OPG, Trap, and CTSK) were up-regulated, while bone formation markers (OCN, ALP, Runx2, SP7, and Col-1) were down-regulated in model group, compared to wild type mice. In vitro, hepcidin1 knockdown inhibited proliferation and osteogenic differentiation, while promoted apoptosis, with increased levels of iron and ferritin. Conclusion Iron accumulation induced by hepcidin1 deficiency aggravates the progression of aging osteoporosis via inhibiting osteogenesis and promoting osteoclast genesis.

الإتاحة: https://doi.org/10.1186/s13018-024-04535-zTest

المؤلفون: Xiong, Lingyun, Liu, Yaru, Zeng, Qing, Lai, JianPing, Qiao, Xiang

المساهمون: National Natural Science Foundation of China, Changsha University of Science and Technology

المصدر: RSC Advances ; volume 14, issue 11, page 7459-7467 ; ISSN 2046-2069

الوصف: The present work investigated the effect of destabilization time on the mechanical properties and microstructure evolution of high chromium cast iron, and scanning electron microscopy and electron probe microanalysis techniques were employed.

الإتاحة: https://doi.org/10.1039/d4ra01247jTest
http://pubs.rsc.org/en/content/articlepdf/2024/RA/D4RA01247JTest

المؤلفون: Zeng, Qing, Li, Caiyue, Yu, Tana, Zhang, Hongchen

المصدر: American Journal of Physical Medicine & Rehabilitation ; ISSN 1537-7385 0894-9115

الوصف: Objective The aim of this network meta-analysis was to compare the improvement effects of various exercise interventions and mindfulness-based interventions to determine the best interventions for the improvement of cognitive impairment. Design 7 databases were searched to screen RCTs of exercise interventions and mindfulness-based interventions to improve cognitive impairment. The network meta-analysis was performed using Revman 5.3, R 4.2.1 and ADDIS 1.16.8 software. Results 34 RCTs involving 14 interventions were included in the study. In terms of cognitive function, except for mindfulness-based stress reduction, all interventions showed significantly greater improvement in cognitive function compared with conventional therapy. Physical activity and Qigong showed better effect in improving executive function. In terms of improving verbal memory, compensatory cognitive training, neurofeedback training, Qigong and sham Qigong were more effective than other interventions. On performing surface under the cumulative ranking curve analysis, acceptance and commitment therapy, neurofeedback training, Qigong, and mediation had the best effects on cognitive function, quality of life, executive function, and processing speed, respectively. Conclusions Mindfulness-based interventions were found to be more effective than exercise interventions for alleviating cognitive impairment. More robust RCTs focusing on acceptance and commitment therapy for cognitive impairment are required to support the current evidence.

الإتاحة: https://doi.org/10.1097/phm.0000000000002531Test

المؤلفون: Cai, Yuxin, Wen, Shuyang, Hu, Jinjing, Wang, Ziyi, Huang, Guozhi, Zeng, Qing, Zou, Jihua

المصدر: Frontiers in Neuroscience ; volume 18 ; ISSN 1662-453X

الوصف: Background Previous evidence suggests a link between gut microbiota and chronic pain, but the causal relationship is not yet fully understood. Methods We categorized gut microbiota based on phylum, class, order, family, and genus levels and gathered pain-related information from the UKB and FinnGen GWAS project. Then, we conducted MR analysis to explore the potential causal relationship between gut microbiota and chronic pain at 12 specific locations. Results We have discovered a direct connection between genetic susceptibility in the gut microbiota (gut metabolites) and pain experienced at 12 specific locations. Notably, Serotonin (5-HT) and Glycine were found to be associated with a higher risk of pain in the extremities. On the other hand, certain microbial families and orders were found to have a protective effect against migraines. Specifically, the family Bifidobacteriaceae (IVW, FDR p = 0.013) was associated with a lower risk of migraines. Furthermore, the genus Oxalobacter (IVW, FDR p = 0.044) was found to be linked to an increased risk of low back pain. Importantly, these associations remained significant even after applying the Benjamini-Hochberg correction test. Our analysis did not find any heterogeneity in the data ( p > 0.05), as confirmed by the Cochrane’s Q -test. Additionally, both the MR-Egger and MR-PRESSO tests indicated no significant evidence of horizontal pleiotropy ( p > 0.05). Conclusion Our MR analysis demonstrated a causal relationship between the gut microbiota and pain, highlighting its potential significance in advancing our understanding of the underlying mechanisms and clinical implications of microbiota-mediated pain.

الإتاحة: https://doi.org/10.3389/fnins.2024.1369996Test

المؤلفون: Chen, Ling, Wan, Yantong, Yang, Tingting, Zhang, Qi, Zeng, Yuting, Zheng, Shuqi, Ling, Zhishan, Xiao, Yupeng, Wan, Qingyi, Liu, Ruili, Yang, Chun, Huang, Guozhi, Zeng, Qing

المصدر: Frontiers in Genetics ; volume 14 ; ISSN 1664-8021

الوصف: Background: Single-cell sequencing (SCS) is a technique used to analyze the genome, transcriptome, epigenome, and other genetic data at the level of a single cell. The procedure is commonly utilized in multiple fields, including neurobiology, immunology, and microbiology, and has emerged as a key focus of life science research. However, a thorough and impartial analysis of the existing state and trends of SCS-related research is lacking. The current study aimed to map the development trends of studies on SCS during the years 2010–2022 through bibliometric software. Methods: Pertinent papers on SCS from 2010 to 2022 were obtained using the Web of Science Core Collection. Research categories, nations/institutions, authors/co-cited authors, journals/co-cited journals, co-cited references, and keywords were analyzed using VOSviewer, the R package “bibliometric”, and CiteSpace. Results: The bibliometric analysis included 9,929 papers published between 2010 and 2022, and showed a consistent increase in the quantity of papers each year. The United States was the source of the highest quantity of articles and citations in this field. The majority of articles were published in the periodical Nature Communications. Butler A was the most frequently quoted author on this topic, and his article “ Integrating single-cell transcriptome data across diverse conditions, technologies, and species ” has received numerous citations to date. The literature and keyword analysis showed that studies involving single-cell RNA sequencing (scRNA-seq) were prominent in this discipline during the study period. Conclusion: This study utilized bibliometric techniques to visualize research in SCS-related domains, which facilitated the identification of emerging patterns and future directions in the field. Current hot topics in SCS research include COVID-19, tumor microenvironment, scRNA-seq, and neuroscience. Our results are significant for scholars seeking to identify key issues and generate new research ideas.

الإتاحة: https://doi.org/10.3389/fgene.2023.1285599Test

المؤلفون: Zeng, Qing-Yue, Qin, Yu, Shi, Yi, Mu, Xing-Yu, Huang, Shi-Jun, Yang, Yu-Hao, Liu, Si-Min, An, Zhen-Mei, Li, Shuang-Qing

المصدر: Frontiers in Immunology ; volume 15 ; ISSN 1664-3224

مصطلحات موضوعية: Immunology, Immunology and Allergy

الوصف: Background Sarcopenia, common in the elderly, often linked to chronic diseases, correlates with inflammation.The association between SII and mortality in sarcopenia patients is underexplored, this study investigates this relationship in a U.S. adult cohort. Methods We analyzed 1999–2018 NHANES data, focusing on 2,974 adults with sarcopenia. Mortality outcomes were determined by linking to National Death Index (NDI) records up to December 31, 2019. Using a weighted sampling design, participants were grouped into three groups by the Systemic Immune-Inflammation Index (SII). We used Cox regression models, adjusting for demographic and clinical variables, to explore SII’s association with all-cause and cause-specific mortality in sarcopenia, performing sensitivity analyses for robustness. Results Over a median follow-up of 9.2 years, 829 deaths occurred. Kaplan-Meier analysis showed significant survival differences across SII groups. The highest SII group showed higher hazard ratios (HRs) for all-cause and cause-specific mortality in both crude and adjusted models. The highest SII group had a higher HR for all-cause(1.57, 1.25–1.98), cardiovascular(1.61, 1.00–2.58), cancer(2.13, 1.32–3.44), and respiratory disease mortality(3.21, 1.66–6.19) in fully adjusted models. Subgroup analyses revealed SII’s association with all-cause mortality across various demographics, including age, gender, and presence of diabetes or cardiovascular disease. Sensitivity analyses, excluding participants with cardiovascular diseases, those who died within two years of follow-up, or those under 45 years of age, largely reflected these results, with the highest SII group consistently demonstrating higher HRs for all types of mortality in both unadjusted and adjusted models. Conclusion Our study is the first to demonstrate a significant relationship between SII and increased mortality risks in a sarcopenia population.

الإتاحة: https://doi.org/10.3389/fimmu.2024.1376544Test

المؤلفون: Wei, Xin, Shi, Zhan-Ming, Lan, Xian-Jun, Qin, Zhen-Juan, Mo, Yu, Wu, Hua-Wang, Huang, Xing-Bing, Zeng, Qing-Bin, Luo, Li-Xia, Yang, Xin-Hu, Zheng, Wei

المصدر: Frontiers in Psychiatry ; volume 14 ; ISSN 1664-0640

مصطلحات موضوعية: Psychiatry and Mental health

الوصف: Background In randomized clinical trials (RCTs) investigating the application of transcranial alternating current stimulation (tACS) in schizophrenia, inconsistent results have been reported. The purpose of this exploratory systematic review of RCTs was to evaluate tACS as an adjunct treatment for patients with schizophrenia based on its therapeutic effects, tolerability, and safety. Methods Our analysis included RCTs that evaluated adjunctive tACS’ effectiveness, tolerability, and safety in schizophrenia patients. Three independent authors extracted data and synthesized it using RevMan 5.3 software. Results Three RCTs involving 76 patients with schizophrenia were encompassed in the analysis, with 40 participants receiving active tACS and 36 receiving sham tACS. Our study revealed a significant superiority of active tACS over sham tACS in improving total psychopathology (standardized mean difference [SMD] = −0.61, 95% confidence interval [CI]: −1.12, −0.10; I 2 = 16%, p = 0.02) and negative psychopathology (SMD = −0.65, 95% CI: −1.11, −0.18; I 2 = 0%, p = 0.007) in schizophrenia. The two groups, however, showed no significant differences in positive psychopathology, general psychopathology, or auditory hallucinations (all p > 0.05). Two RCTs examined the neurocognitive effects of tACS, yielding varied findings. Both groups demonstrated similar rates of discontinuation due to any reason and adverse events (all p > 0.05). Conclusion Adjunctive tACS is promising as a viable approach for mitigating total and negative psychopathology in individuals diagnosed with schizophrenia. However, to gain a more comprehensive understanding of tACS’s therapeutic effects in schizophrenia, it is imperative to conduct extensive, meticulously planned, and well-documented RCTs.

الإتاحة: https://doi.org/10.3389/fpsyt.2023.1308437Test