دورية أكاديمية
Application of LDH assay for therapeutic efficacy evaluation of ex vivo tumor models
| العنوان: | Application of LDH assay for therapeutic efficacy evaluation of ex vivo tumor models |
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| المؤلفون: | Megan C. Cox, Rita Mendes, Fernanda Silva, Teresa F. Mendes, Adelyn Zelaya-Lazo, Kathleen Halwachs, Julie J. Purkal, Inês A. Isidro, Ana Félix, Erwin R. Boghaert, Catarina Brito |
| المصدر: | Scientific Reports, Vol 11, Iss 1, Pp 1-14 (2021) |
| بيانات النشر: | Nature Portfolio |
| سنة النشر: | 2021 |
| المجموعة: | Directory of Open Access Journals: DOAJ Articles |
| مصطلحات موضوعية: | Medicine, Science |
| الوصف: | The current standard preclinical oncology models are not able to fully recapitulate therapeutic targets and clinically relevant disease biology, evidenced by the 90% attrition rate of new therapies in clinical trials. Three-dimensional (3D) culture systems have the potential to enhance the relevance of preclinical models. However, the limitations of currently available cellular assays to accurately evaluate therapeutic efficacy in these models are hindering their widespread adoption. We assessed the compatibility of the lactate dehydrogenase (LDH) assay in 3D spheroid cultures against other commercially available readout methods. We developed a standardized protocol to apply the LDH assay to ex vivo cultures, considering the impact of culture growth dynamics. We show that accounting for growth rates and background release levels of LDH are sufficient to make the LDH assay a suitable methodology for longitudinal monitoring and endpoint assessment of therapeutic efficacy in both cell line-derived xenografts (xenospheres) and patient-derived explant cultures. This method has the added value of being non-destructive and not dependent on reagent penetration or manipulation of the parent material. The establishment of reliable readout methods for complex 3D culture systems will further the utility of these tumor models in preclinical and co-clinical drug development studies. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 2045-2322 |
| العلاقة: | https://doi.org/10.1038/s41598-021-97894-0Test; https://doaj.org/toc/2045-2322Test; https://doaj.org/article/6ba6021cf4734f4ebc89722a5ae55c02Test |
| DOI: | 10.1038/s41598-021-97894-0 |
| الإتاحة: | https://doi.org/10.1038/s41598-021-97894-0Test https://doaj.org/article/6ba6021cf4734f4ebc89722a5ae55c02Test |
| رقم الانضمام: | edsbas.B260C1C9 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 20452322 |
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| DOI: | 10.1038/s41598-021-97894-0 |