المؤلفون: Chiyako Touge, Michiyo Nakatsu, Mai Sugimoto, Eiichiro Takamura, Hiroaki Sakamoto

مصطلحات موضوعية: Biophysics, Biochemistry, Medicine, Microbiology, Molecular Biology, Biotechnology, Infectious Diseases, Space Science, Environmental Sciences not elsewhere classified, Biological Sciences not elsewhere classified, Chemical Sciences not elsewhere classified, reach 20 na, lb liquid medium, carbon comb structure, carbon comb electrodes, 1 – 10, novel biochemical sensor, living escherichia coli, biochemical corrosion monitoring, bacterial count range, 20 , 2 , c sensor increased, measures galvanic current, coli solution increases, 8 , bacterial solution, c sensor, coli count, galvanic current

الوصف: For the detection and monitoring of live bacteria, we propose a biochemical corrosion monitoring (BCM) sensor that measures galvanic current by using a Ag/C sensor comprising silver and carbon comb electrodes. The deposition of an Escherichia coli suspension containing an LB liquid medium on the Ag/C sensor increased the galvanic current. The time required for the current to reach 20 nA is defined as T 20 . T 20 tends to decrease as the initial number of E. coli in the E. coli solution increases. A linear relationship was obtained between the logarithm of the E. coli count and T 20 in a bacterial count range of 1–10 8 cfu/mL under culture conditions in which the growth rate of the bacteria was constant. Hence, the number of live E. coli could be determined from T 20 . Ag 2 S precipitation was observed on the surface of the Ag electrode of the Ag/C sensor, where an increase in the current was observed. This generation of galvanic current was attributed to the reaction between a small amount of free H 2 S metabolized by E. coli in the bacterial solution during its growth process and Agthe sensor anode. The Ag/C sensor can detect a free H 2 S concentration of 0.041 μM in the E. coli solution. This novel biochemical sensor can monitor the growth behavior of living organisms without damaging them.

العلاقة: https://figshare.com/articles/journal_contribution/A_Biochemical_Corrosion_Monitoring_Sensor_with_a_Silver_Carbon_Comb_Structure_for_the_Detection_of_Living_Escherichia_coli/24550828Test

الإتاحة: https://doi.org/10.1021/acsomega.3c03632.s001Test
https://figshare.com/articles/journal_contribution/A_Biochemical_Corrosion_Monitoring_Sensor_with_a_Silver_Carbon_Comb_Structure_for_the_Detection_of_Living_Escherichia_coli/24550828Test

المؤلفون: Misao Nishikawa, Toru Yamagata, Kentarou Naito, Noritsugu Kunihiro, Hiroaki Sakamoto, Mistuhiro Hara, Kenji Ohata, Takeo Goto

المصدر: Journal of Clinical Medicine; Volume 11; Issue 15; Pages: 4556

مصطلحات موضوعية: Chiari malformation, syringomyelia, surgery, clinical outcome, cerebrospinal fluid, cerebrospinal fluid flow dynamics, posterior fossa decompression

الوصف: Introduction: The mainstay of treatment of syringomyelia associated with Chiari malformation type I (CM-I) is the management of CM-I to normalize the cerebrospinal fluid (CSF) flow at the foramen magnum. CM-I is classified into three independent types. Surgical treatment was selected based on the mechanism of hindbrain ptosis in each CM-I type. Materials and Methods: Foramen magnum decompression (FMD: 213 cases), expansive suboccipital cranioplasty (ESCP: 87 cases), and craniocervical fixation (CCF: 30 cases) were performed. CSF flow dynamics were assessed pre- and post-surgery using cine phase contrast magnetic resonance imaging. During surgery, CSF flow dynamics were examined using color Doppler ultrasonography (CDU). Results: ESCP and FMD demonstrated high rates of improvement in neurological symptoms and signs (82.7%), whereas CCF demonstrated a high rate of improvement in neurological symptoms (89%). The pre-operative maximum flow velocity (cm/s) was significantly lower in patients than in controls and increased post-operatively. During surgery, CDU indicated that the volume of the major cistern was 8 mL, and the maximum flow velocity was >3 mL/s. Conclusions: An appropriate surgical treatment should be selected for CM-I to correct hindbrain ptosis. In addition, it is necessary to confirm the normalization of CSF flow at the foramen of Magendie.

وصف الملف: application/pdf

العلاقة: Clinical Neurology; https://dx.doi.org/10.3390/jcm11154556Test

الإتاحة: https://doi.org/10.3390/jcm11154556Test

المؤلفون: Hisashi Tanaka, Hiroaki Sakamoto, Takahiro Akita, Fumiyoshi Ohyanagi, Yosuke Kawashima, Yuichi Tambo, Azusa Tanimoto, Atsushi Horiike, Eisaku Miyauchi, Yuko Tsuchiya‐Kawano, Noriko Yanagitani, Makoto Nishio

المصدر: Thoracic Cancer, Vol 13, Iss 10, Pp 1471-1478 (2022)

مصطلحات موضوعية: dacomitinib, epidermal growth factor receptor mutation, exon 21 L858R, non‐small cell lung cancer, rechallenge, Neoplasms. Tumors. Oncology. Including cancer and carcinogens, RC254-282

الوصف: Background Dacomitinib is the second‐generation epidermal growth factor receptor (EGFR)‐tyrosine kinase inhibitor (TKI) for mutant non–small cell lung cancer (NSCLC). EGFR‐TKIs are often re‐administered in Japan after the disease progression prior EGFR‐TKI. There is little evidence of dacomitinib in rechallenge setting. This study evaluated clinical outcomes of dacomitinib in rechallenge setting. Methods Patients who received dacomitinib for advanced EGFR‐mutant NSCLC who had progressed after EGFR‐TKI in nine institutions in Japan were included in the analyses. Results In total, 43 patients were analyzed. The median progression‐free survival (PFS) was 4.3 months (95% confidence interval [CI], 2.5–5.6). The overall survival (OS) was 10.5 months (95% CI, 7.4–not reached). The overall response rate was 25.5% (95% CI, 13.1–33.7). Subset analysis indicated that patients with EGFR exon 21 L858R showed longer PFS than those with EGFR exon 19 deletion (5.8 vs. 4.1 months) (p = 0.018). The most common adverse events leading to dose modification were diarrhea, paronychia, rash, and oral mucositis. Conclusion In the real practice in Japan, dacomitinib showed a worthwhile treatment option for NSCLC patients with EGFR mutation after failure of previous EGFR‐TKI. The benefit was especially pronounced in patients with the exon 21 mutation.

العلاقة: https://doi.org/10.1111/1759-7714.14415Test; https://doaj.org/toc/1759-7706Test; https://doaj.org/toc/1759-7714Test; https://doaj.org/article/5e5f715847fd4a66aa35dd6d0e976bdaTest

الإتاحة: https://doi.org/10.1111/1759-7714.14415Test
https://doaj.org/article/5e5f715847fd4a66aa35dd6d0e976bdaTest

المؤلفون: Misao Nishikawa, Toru Yamagata, Kentarou Naito, Noritsugu Kunihiro, Hiroaki Sakamoto, Mistuhiro Hara, Kenji Ohata, Takeo Goto

المصدر: Journal of Clinical Medicine, Vol 11, Iss 4556, p 4556 (2022)

مصطلحات موضوعية: Chiari malformation, syringomyelia, surgery, clinical outcome, cerebrospinal fluid, cerebrospinal fluid flow dynamics, Medicine

الوصف: Introduction: The mainstay of treatment of syringomyelia associated with Chiari malformation type I (CM-I) is the management of CM-I to normalize the cerebrospinal fluid (CSF) flow at the foramen magnum. CM-I is classified into three independent types. Surgical treatment was selected based on the mechanism of hindbrain ptosis in each CM-I type. Materials and Methods: Foramen magnum decompression (FMD: 213 cases), expansive suboccipital cranioplasty (ESCP: 87 cases), and craniocervical fixation (CCF: 30 cases) were performed. CSF flow dynamics were assessed pre- and post-surgery using cine phase contrast magnetic resonance imaging. During surgery, CSF flow dynamics were examined using color Doppler ultrasonography (CDU). Results: ESCP and FMD demonstrated high rates of improvement in neurological symptoms and signs (82.7%), whereas CCF demonstrated a high rate of improvement in neurological symptoms (89%). The pre-operative maximum flow velocity (cm/s) was significantly lower in patients than in controls and increased post-operatively. During surgery, CDU indicated that the volume of the major cistern was 8 mL, and the maximum flow velocity was >3 mL/s. Conclusions: An appropriate surgical treatment should be selected for CM-I to correct hindbrain ptosis. In addition, it is necessary to confirm the normalization of CSF flow at the foramen of Magendie.

العلاقة: https://www.mdpi.com/2077-0383/11/15/4556Test; https://doaj.org/toc/2077-0383Test; https://doaj.org/article/ef37bd8f5883456b97ed53b32ce48ef0Test

الإتاحة: https://doi.org/10.3390/jcm11154556Test
https://doaj.org/article/ef37bd8f5883456b97ed53b32ce48ef0Test

المؤلفون: Misao NISHIKAWA, Paolo A. BOLOGNESE, Toru YAMAGATA, Kentarou NAITO, Hiroaki SAKAMOTO, Mistuhiro HARA, Kenji OHATA, Takeo GOTO

المصدر: Neurologia Medico-Chirurgica, Vol 62, Iss 9, Pp 400-415 (2022)

مصطلحات موضوعية: chiari malformation, craniocervical junction, instability, pathogenesis, surgical intervention, Neurosciences. Biological psychiatry. Neuropsychiatry, RC321-571

الوصف: We investigated the mechanism underlying Chiari malformation type I (CM-I) and classified it according to the morphometric analyses of posterior cranial fossa (PCF) and craniocervical junction (CCJ). Three independent subtypes of CM-I were confirmed (CM-I types A, B, and C) for 484 cases and 150 normal volunteers by multiple analyses. CM-I type A had normal volume of PCF (VPCF) and occipital bone size. Type B had normal VPCF and small volume of the area surrounding the foramen magnum (VAFM) and occipital bone size. Type C had small VPCF, VAFM, and occipital bone size. Morphometric analyses during craniocervical traction test demonstrated instability of CCJ. Foramen magnum decompression (FMD) was performed in 302 cases. Expansive suboccipital cranioplasty (ESCP) was performed in 102 cases. Craniocervical posterolateral fixation (CCF) was performed for CCJ instability in 70 cases. Both ESCP and FMD showed a high improvement rate of neurological symptoms and signs (84.4%) and a high recovery rate of the Japanese Orthopaedic Association (JOA) score (58.5%). CCF also showed a high recovery rate of the JOA score (69.7%), with successful joint stabilization (84.3%). CM-I type A was associated with other mechanisms that caused ptosis of the brainstem and cerebellum (CCJ instability and traction and pressure dissociation between the intracranial cavity and spinal canal cavity), whereas CM-I types B and C demonstrated underdevelopment of the occipital bone. For CM-I types B and C, PCF decompression should be performed, whereas for small VPCF, ESCP should be performed. CCF for CCJ instability (including CM-I type A) was safe and effective.

العلاقة: https://www.jstage.jst.go.jp/article/nmc/62/9/62_2022-0078/_pdf/-char/enTest; https://doaj.org/toc/1349-8029Test; https://doaj.org/article/acf990ccbf6c490196d031d7bfdb0a4eTest

الإتاحة: https://doi.org/10.2176/jns-nmc.2022-0078Test
https://doaj.org/article/acf990ccbf6c490196d031d7bfdb0a4eTest

المؤلفون: Chiyako TOUGE, Michiyo NAKATSU, Mai SUGIMOTO, Hiroaki SAKAMOTO

مصطلحات موضوعية: Chemistry, Electrochemical Sensor, Ag Electrode, Galvanic current, H2S

الوصف: Given that trace level H 2 S quantitation in biological samples currently requires expensive and time-consuming instrumental procedures, we herein developed an electrochemical Ag/C sensor for the detection of H 2 S in protein-containing neutral solutions. The detection principle was based on the increase in galvanic current that was elicited by the sulfidation of the Ag electrode and was linearly dependent on the concentration of H 2 S. This linear relationship enabled the quantitation of H 2 S in the concentration range of 0.037–6.7 µmol L −1 in mock biological samples without any pretreatment, highlighting the potential of the Ag/C sensor for the analysis of water quality and food, blood, and other biological samples.

العلاقة: https://figshare.com/articles/dataset/Electrochemical_Sensing_of_Hydrogen_Sulfide_Traces_in_Biological_Samples_Supporting_Information_/24785187Test

الإتاحة: https://doi.org/10.50892/data.electrochemistry.24785187.v1Test
https://figshare.com/articles/dataset/Electrochemical_Sensing_of_Hydrogen_Sulfide_Traces_in_Biological_Samples_Supporting_Information_/24785187Test

المؤلفون: Tomohiro Komatsu, Kazuki Hishii, Michiko Kimura, Satoshi Amaya, Hiroaki Sakamoto, Eiichiro Takamura, Takenori Satomura, Shin-ichiro Suye

المصدر: International Journal of Molecular Sciences; Volume 22; Issue 24; Pages: 13503

مصطلحات موضوعية: biofuel cells, bioanode, multi-step cascade reactions, efficient electron transfer, microfluidic system, immobilized enzymes method

جغرافية الموضوع: agris

الوصف: With the rapid decline of fossil fuels, various types of biofuel cells (BFCs) are being developed as an alternative energy source. BFCs based on multi-enzyme cascade reactions are utilized to extract more electrons from substrates. Thus, more power density is obtained from a single molucule of substrate. In the present study, a bioanode that could extract six electrons from a single molecule of L-proline via a three-enzyme cascade reaction was developed and investigated for its possible use in BFCs. These enzymes were immobilized on the electrode to ensure highly efficient electron transfer. Then, oriented immobilization of enzymes was achieved using two types of self-assembled monolayers (SAMs). In addition, a microfluidic system was incorporated to achieve efficient electron transfer. The microfluidic system, in which the electrodes were arranged in a tooth-shaped comb, allowed for substrates to be supplied continuously to the cascade, which resulted in smooth electron transfer. Finally, we developed a high-performance bioanode which resulted in the accumulation of higher current density compared to that of a gold disc electrode (205.8 μA cm−2: approximately 187 times higher). This presents an opportunity for using the bioanode to develop high-performance BFCs in the future.

وصف الملف: application/pdf

العلاقة: Biochemistry; https://dx.doi.org/10.3390/ijms222413503Test

الإتاحة: https://doi.org/10.3390/ijms222413503Test

المؤلفون: Hiroaki Sakamoto, Noriko Yanagitani, Ryo Manabe, Ryosuke Tsugitomi, Shinsuke Ogusu, Takehiro Tozuka, Hiroshi Yoshida, Yoshiaki Amino, Ryo Ariyasu, Ken Uchibori, Satoru Kitazono, Sadatomo Tasaka, Makoto Nishio

المصدر: Cancer Reports, Vol 4, Iss 6, Pp n/a-n/a (2021)

مصطلحات موضوعية: alectinib, CNS metastases, crizotinib, non‐small cell lung cancer, Neoplasms. Tumors. Oncology. Including cancer and carcinogens, RC254-282

الوصف: Background Most patients treated with anaplastic lymphoma kinase (ALK)‐tyrosine kinase inhibitors for ALK‐positive non‐small cell lung cancer (NSCLC) develop resistance, leading to metastasis, with progression to the central nervous system (CNS) being a primary concern. Although alectinib has better CNS penetration than crizotinib, patients treated with alectinib also develop CNS progression. CNS metastases more likely occurs during crizotinib treatment due to less blood‐brain barrier (BBB) penetration capability than alectinib. CNS progression pattern may be different during crizotinib and alecitinib treatment. Understanding the characteristics of CNS progression is important for developing treatment strategies. Aims We compared the clinical‐radiographic characteristics of CNS metastases among patients undergoing crizotinib and alectinib treatment for ALK‐positive NSCLCs. Methods and results We retrospectively analyzed the radiographic and clinical characteristics of CNS progression in ALK‐positive NSCLC patients treated with crizotinib or alectinib at our hospital between July 2011 and May 2020. CNS and systemic tumor progression were evaluated using computed tomography or magnetic resonance imaging. Fifty‐three and 65 patients were treated with crizotinib and alectinib, respectively. Baseline CNS metastasis was observed in 18 and 27 patients in the crizotinib and alectinib groups, respectively. Among the patients in the crizotinib and alectinib groups who developed disease progression, 15/49 (30.6%) and 9/44 (20.5%) had CNS progression, respectively (P = .344). Intra‐CNS progression‐free survival was significantly longer in the alectinib group than in the crizotinib group (median: 14.0 vs 5.6 months, P = .042). The number of CNS metastases sized ≥3 cm, rate of peritumoral brain edema, and the second progression pattern after treatment continuation was not significantly different between the groups. Conclusion We observed no significant difference in the clinical‐radiographic characteristics of CNS ...

العلاقة: https://doi.org/10.1002/cnr2.1414Test; https://doaj.org/toc/2573-8348Test; https://doaj.org/article/e37385b3c00c43baa5109687ecafc390Test

الإتاحة: https://doi.org/10.1002/cnr2.1414Test
https://doaj.org/article/e37385b3c00c43baa5109687ecafc390Test

المؤلفون: Masamichi Itoga, Hisashi Tanaka, Kageaki Taima, Yoshiko Ishioka, Hiroaki Sakamoto, Shingo Takanashi, Akira Kurose, Sadatomo Tasaka

المصدر: BMC Research Notes, Vol 14, Iss 1, Pp 1-5 (2021)

مصطلحات موضوعية: Carcinoid, Immunohistochemistry, Large cell neuroendocrine carcinoma, Procalcitonin, Pulmonary neuroendocrine tumor, Medicine, Biology (General), QH301-705.5, Science (General), Q1-390

الوصف: Objective Procalcitonin (PCT) has received much attention as a serum marker for bacterial infection. Elevated serum PCT is occasionally seen in severe trauma, heatstroke, and neoplastic diseases, including lung cancer with neuroendocrine component. Results In the present study, we evaluated PCT expression in the specimen of pulmonary neuroendocrine tumors, comparing large cell neuroendocrine carcinoma (LCNEC), carcinoid, and small cell lung carcinoma (SCLC). Pathological specimens of 10 LCNEC, 4 carcinoid, and 7 SCLC cases were evaluated with immunochemical staining of PCT. Clinical characteristics and serum levels of PCT and C-reactive protein were also evaluated. We observed positive PCT expression in 5 (50%) LCNEC and 2 (50%) carcinoid specimens that were surgically resected. Whereas serum PCT levels were not elevated in patients with PCT-positive carcinoid, two out of three LCNEC patients with high PCT expression in the tumor had elevated serum PCT levels that reflected disease progression. In patients with SCLC, PCT was not detected in the tumor or serum. This is the first immunohistochemical study of the PCT expression in the lung tumor specimens. We concluded that, in patients with LCNEC, high serum PCT levels may be indicative of disease activity and serve as a biomarker.

العلاقة: https://doi.org/10.1186/s13104-021-05448-4Test; https://doaj.org/toc/1756-0500Test; https://doaj.org/article/f69d4cd0ac01440686947a29c822fddfTest

الإتاحة: https://doi.org/10.1186/s13104-021-05448-4Test
https://doaj.org/article/f69d4cd0ac01440686947a29c822fddfTest

المؤلفون: Tomohiro Komatsu, Kazuki Hishii, Michiko Kimura, Satoshi Amaya, Hiroaki Sakamoto, Eiichiro Takamura, Takenori Satomura, Shin-ichiro Suye

المصدر: International Journal of Molecular Sciences, Vol 22, Iss 13503, p 13503 (2021)

مصطلحات موضوعية: biofuel cells, bioanode, multi-step cascade reactions, efficient electron transfer, microfluidic system, immobilized enzymes method, Biology (General), QH301-705.5, Chemistry, QD1-999

الوصف: With the rapid decline of fossil fuels, various types of biofuel cells (BFCs) are being developed as an alternative energy source. BFCs based on multi-enzyme cascade reactions are utilized to extract more electrons from substrates. Thus, more power density is obtained from a single molucule of substrate. In the present study, a bioanode that could extract six electrons from a single molecule of L-proline via a three-enzyme cascade reaction was developed and investigated for its possible use in BFCs. These enzymes were immobilized on the electrode to ensure highly efficient electron transfer. Then, oriented immobilization of enzymes was achieved using two types of self-assembled monolayers (SAMs). In addition, a microfluidic system was incorporated to achieve efficient electron transfer. The microfluidic system, in which the electrodes were arranged in a tooth-shaped comb, allowed for substrates to be supplied continuously to the cascade, which resulted in smooth electron transfer. Finally, we developed a high-performance bioanode which resulted in the accumulation of higher current density compared to that of a gold disc electrode (205.8 μA cm −2 : approximately 187 times higher). This presents an opportunity for using the bioanode to develop high-performance BFCs in the future.

العلاقة: https://www.mdpi.com/1422-0067/22/24/13503Test; https://doaj.org/toc/1661-6596Test; https://doaj.org/toc/1422-0067Test; https://doaj.org/article/62c854a8e06d4df5bd08f6529e4d83d0Test

الإتاحة: https://doi.org/10.3390/ijms222413503Test
https://doaj.org/article/62c854a8e06d4df5bd08f6529e4d83d0Test