دورية أكاديمية
Evaluation of antidiabetic effect of Cissampelos pareira L. (Menispermaceae) root extract in streptozotocin-nicotinamide-induced diabetic rats via targeting SGLT2 inhibition
| العنوان: | Evaluation of antidiabetic effect of Cissampelos pareira L. (Menispermaceae) root extract in streptozotocin-nicotinamide-induced diabetic rats via targeting SGLT2 inhibition |
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| المؤلفون: | Ankit Kumar, Ravindra Semwal, Ashutosh Chauhan, Ruchi Badoni Semwal, Subhash Chandra, Debabrata Sircar, Partha Roy, Deepak Kumar Semwal |
| المصدر: | Phytomedicine Plus, Vol 2, Iss 4, Pp 100374- (2022) |
| بيانات النشر: | Elsevier |
| سنة النشر: | 2022 |
| المجموعة: | Directory of Open Access Journals: DOAJ Articles |
| مصطلحات موضوعية: | Diabetes mellitus, Lipid profile, Liver function test, Kidney function test, Ayurveda, Other systems of medicine, RZ201-999 |
| الوصف: | Background: Cissampelos pareira L. (Menispermaceae) has been used in Indian traditional medicine for a variety of human ailments including diabetes mellitus. Although its anti-hyperglycemic activity has been validated by previous studies, a detailed mechanism of action and the responsible components are still unknown. Purpose: The present study aimed to evaluate the antidiabetic effect of aqueous-ethanolic extract of C. pareira roots in streptozotocin-nicotinamide (STZ-NAM)-induced diabetic rats via targeting SGLT2 inhibition. Methods: In vitro α-amylase and α-glucosidase inhibitory assays were conducted using Epoch Microplate Spectrophotometer. A-498 cells viability assay and SGLT2 protein expression study were conducted using MTT assay and western blot respectively. STZ-NAM-induced diabetic rats were used as a model to evaluate the antidiabetic effect of the extract. Selected bioactive compounds reported earlier from the plant were studied for their in silico SGLT2 inhibitory activity. Results: The extract showed α-amylase and α-glucosidase inhibitory effects with IC50 values of 18.0 ± 1.01 and 4.87±0.54 mg/mL, respectively. A-498 cells viability assay showed its CC50 value of 0.8 mg/mL. The extract showed a notable effect on SGLT2 protein expression. The in vivo study revealed that the extract significantly (p<0.05) reduced the glucose levels in rats at the dose of 500 mg/kg, b.w. in a 28-days study. The molecular docking studies revealed insulanoline and warifteine as the most active compounds with docking scores of -10 and -9.9 kcal mol−1, respectively. Conclusion: The study showed that the extract has the potential to reduce STZ-induced elevated blood glucose without affecting liver and kidney functions. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 2667-0313 |
| العلاقة: | http://www.sciencedirect.com/science/article/pii/S2667031322001531Test; https://doaj.org/toc/2667-0313Test; https://doaj.org/article/2984e4f8d1d245e5a8f9e19047e581a9Test |
| DOI: | 10.1016/j.phyplu.2022.100374 |
| الإتاحة: | https://doi.org/10.1016/j.phyplu.2022.100374Test https://doaj.org/article/2984e4f8d1d245e5a8f9e19047e581a9Test |
| رقم الانضمام: | edsbas.428A5085 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 26670313 |
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| DOI: | 10.1016/j.phyplu.2022.100374 |