دورية أكاديمية
Prime-seq, efficient and powerful bulk RNA sequencing
| العنوان: | Prime-seq, efficient and powerful bulk RNA sequencing |
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| المؤلفون: | Aleksandar Janjic, Lucas E. Wange, Johannes W. Bagnoli, Johanna Geuder, Phong Nguyen, Daniel Richter, Beate Vieth, Binje Vick, Irmela Jeremias, Christoph Ziegenhain, Ines Hellmann, Wolfgang Enard |
| المصدر: | Genome Biology, Vol 23, Iss 1, Pp 1-27 (2022) |
| بيانات النشر: | BMC |
| سنة النشر: | 2022 |
| المجموعة: | Directory of Open Access Journals: DOAJ Articles |
| مصطلحات موضوعية: | RNA-seq, Transcriptomics, Genomics, Power analysis, Biology (General), QH301-705.5, Genetics, QH426-470 |
| الوصف: | Cost-efficient library generation by early barcoding has been central in propelling single-cell RNA sequencing. Here, we optimize and validate prime-seq, an early barcoding bulk RNA-seq method. We show that it performs equivalently to TruSeq, a standard bulk RNA-seq method, but is fourfold more cost-efficient due to almost 50-fold cheaper library costs. We also validate a direct RNA isolation step, show that intronic reads are derived from RNA, and compare cost-efficiencies of available protocols. We conclude that prime-seq is currently one of the best options to set up an early barcoding bulk RNA-seq protocol from which many labs would profit. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 1474-760X |
| العلاقة: | https://doi.org/10.1186/s13059-022-02660-8Test; https://doaj.org/toc/1474-760XTest; https://doaj.org/article/68399bd59d28488cb10eea8edd8b0b09Test |
| DOI: | 10.1186/s13059-022-02660-8 |
| الإتاحة: | https://doi.org/10.1186/s13059-022-02660-8Test https://doaj.org/article/68399bd59d28488cb10eea8edd8b0b09Test |
| رقم الانضمام: | edsbas.D9F564D1 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 1474760X |
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| DOI: | 10.1186/s13059-022-02660-8 |