المؤلفون: Carette, Diane, Perrard, Marie-Hélène, Prisant, Nadia, Gilleron, Jérôme, Pointis, Georges, Segretain, Dominique, Durand, Philippe

المساهمون: Université de Nice Sophia-Antipolis (UNSA), UMR S775, Université Paris Descartes - Paris 5 (UPD5), Institut de Génomique Fonctionnelle de Lyon (IGFL), École normale supérieure de Lyon (ENS de Lyon)-Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon, Université de Versailles Saint-Quentin-en-Yvelines (UVSQ), Kallistem SAS, Partenaires INRAE

المصدر: ISSN: 0041-008X.

مصطلحات موضوعية: Hexavalent chromium, Blood-testis barrier, Spermatogenesis, The rat seminiferous tubule culture model, IN-VITRO, CADMIUM CHLORIDE, PROLIFERATION, EPITHELIUM, Junctional proteins, INTERCELLULAR COMMUNICATION, SPERMATOGENIC CELLS, EXPRESSION, EXPOSURE, SYSTEM, [SDV]Life Sciences [q-bio], [INFO]Computer Science [cs]

الوصف: International audience ; Exposure to toxic metals, specifically those belonging to the nonessential group leads to human health defects and among them reprotoxic effects. The mechanisms by which these metals produce their negative effects on spermatogenesis have not been fully elucidated. By using the Durand's validated seminiferous tubule culture model, which mimics the in vivo situation, we recently reported that concentrations of hexavalent chromium, reported in the literature to be closed to that found in the blood circulation of men, increase the number of germ cell cytogenetic abnormalities. Since this metal is also known to affect cellular junctions, we investigated, in the present study, its potential influence on the Sertoli cell barrier and on junctional proteins present at this level such as connexin 43, claudin-11 and N-cadherin. Cultured seminiferous tubules in bicameral chambers expressed the three junctional proteins and ZO-1 for at least 12 days. Exposure to low concentrations of chromium (10 mu g/l) increased the trans-epithelial resistance without major changes of claudin-11 and N-cadherin expressions but strongly delocalized the gap junction protein connexin 43 from the membrane to the cytoplasm of Sertoli cells. The possibility that the hexavalent chromium-induced alteration of connexin 43 indirectly mediates the effect of the toxic metal on the blood testis barrier dynamic is postulated. (c) 2013 Elsevier Inc. All rights reserved.

العلاقة: hal-02651875; https://hal.inrae.fr/hal-02651875Test; PRODINRA: 209151; WOS: 000316305600003

الإتاحة: https://doi.org/10.1016/j.taap.2013.01.016Test
https://hal.inrae.fr/hal-02651875Test

المؤلفون: Carette, Diane, Perrard, Marie-Hélène, Prisant, Nadia, Gilleron, Jérôme, Pointis, Georges, Segretain, Dominique, Durand, Philippe

المساهمون: Université de Nice Sophia-Antipolis (UNSA), UMR S775, Université Paris Descartes - Paris 5 (UPD5), Institut de Génomique Fonctionnelle de Lyon (IGFL), École normale supérieure de Lyon (ENS de Lyon)-Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon, Université de Versailles Saint-Quentin-en-Yvelines (UVSQ), Kallistem SAS, Partenaires INRAE

المصدر: ISSN: 0041-008X.

مصطلحات موضوعية: Hexavalent chromium, Blood-testis barrier, Spermatogenesis, The rat seminiferous tubule culture model, IN-VITRO, CADMIUM CHLORIDE, PROLIFERATION, EPITHELIUM, Junctional proteins, INTERCELLULAR COMMUNICATION, SPERMATOGENIC CELLS, EXPRESSION, EXPOSURE, SYSTEM, [SDV]Life Sciences [q-bio], [INFO]Computer Science [cs]

الوصف: International audience ; Exposure to toxic metals, specifically those belonging to the nonessential group leads to human health defects and among them reprotoxic effects. The mechanisms by which these metals produce their negative effects on spermatogenesis have not been fully elucidated. By using the Durand's validated seminiferous tubule culture model, which mimics the in vivo situation, we recently reported that concentrations of hexavalent chromium, reported in the literature to be closed to that found in the blood circulation of men, increase the number of germ cell cytogenetic abnormalities. Since this metal is also known to affect cellular junctions, we investigated, in the present study, its potential influence on the Sertoli cell barrier and on junctional proteins present at this level such as connexin 43, claudin-11 and N-cadherin. Cultured seminiferous tubules in bicameral chambers expressed the three junctional proteins and ZO-1 for at least 12 days. Exposure to low concentrations of chromium (10 mu g/l) increased the trans-epithelial resistance without major changes of claudin-11 and N-cadherin expressions but strongly delocalized the gap junction protein connexin 43 from the membrane to the cytoplasm of Sertoli cells. The possibility that the hexavalent chromium-induced alteration of connexin 43 indirectly mediates the effect of the toxic metal on the blood testis barrier dynamic is postulated. (c) 2013 Elsevier Inc. All rights reserved.

العلاقة: hal-02651875; https://hal.inrae.fr/hal-02651875Test; PRODINRA: 209151; WOS: 000316305600003

الإتاحة: https://doi.org/10.1016/j.taap.2013.01.016Test
https://hal.inrae.fr/hal-02651875Test

المؤلفون: Carette, Diane, Perrard, Marie-Hélène, Prisant, Nadia, Gilleron, Jérôme, Pointis, Georges, Segretain, Dominique, Durand, Philippe

المساهمون: Université de Nice Sophia-Antipolis (UNSA), UMR S775, Université Paris Descartes - Paris 5 (UPD5), Institut de Génomique Fonctionnelle de Lyon (IGFL), École normale supérieure de Lyon (ENS de Lyon)-Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon, Université de Versailles Saint-Quentin-en-Yvelines (UVSQ), Kallistem SAS, Partenaires INRAE

المصدر: ISSN: 0041-008X.

مصطلحات موضوعية: Hexavalent chromium, Blood-testis barrier, Spermatogenesis, The rat seminiferous tubule culture model, IN-VITRO, CADMIUM CHLORIDE, PROLIFERATION, EPITHELIUM, Junctional proteins, INTERCELLULAR COMMUNICATION, SPERMATOGENIC CELLS, EXPRESSION, EXPOSURE, SYSTEM, [SDV]Life Sciences [q-bio], [INFO]Computer Science [cs]

الوصف: International audience ; Exposure to toxic metals, specifically those belonging to the nonessential group leads to human health defects and among them reprotoxic effects. The mechanisms by which these metals produce their negative effects on spermatogenesis have not been fully elucidated. By using the Durand's validated seminiferous tubule culture model, which mimics the in vivo situation, we recently reported that concentrations of hexavalent chromium, reported in the literature to be closed to that found in the blood circulation of men, increase the number of germ cell cytogenetic abnormalities. Since this metal is also known to affect cellular junctions, we investigated, in the present study, its potential influence on the Sertoli cell barrier and on junctional proteins present at this level such as connexin 43, claudin-11 and N-cadherin. Cultured seminiferous tubules in bicameral chambers expressed the three junctional proteins and ZO-1 for at least 12 days. Exposure to low concentrations of chromium (10 mu g/l) increased the trans-epithelial resistance without major changes of claudin-11 and N-cadherin expressions but strongly delocalized the gap junction protein connexin 43 from the membrane to the cytoplasm of Sertoli cells. The possibility that the hexavalent chromium-induced alteration of connexin 43 indirectly mediates the effect of the toxic metal on the blood testis barrier dynamic is postulated. (c) 2013 Elsevier Inc. All rights reserved.

العلاقة: hal-02651875; https://hal.inrae.fr/hal-02651875Test; PRODINRA: 209151; WOS: 000316305600003

الإتاحة: https://doi.org/10.1016/j.taap.2013.01.016Test
https://hal.inrae.fr/hal-02651875Test

المؤلفون: Carette, Diane, Perrard, Marie-Hélène, Prisant, Nadia, Gilleron, Jérôme, Pointis, Georges, Segretain, Dominique, Durand, Philippe

المساهمون: Université de Nice Sophia-Antipolis (UNSA), UMR S775, Université Paris Descartes - Paris 5 (UPD5), Institut de Génomique Fonctionnelle de Lyon (IGFL), École normale supérieure de Lyon (ENS de Lyon)-Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon, Université de Versailles Saint-Quentin-en-Yvelines (UVSQ), Kallistem SAS, Partenaires INRAE

المصدر: ISSN: 0041-008X.

مصطلحات موضوعية: Hexavalent chromium, Blood-testis barrier, Spermatogenesis, The rat seminiferous tubule culture model, IN-VITRO, CADMIUM CHLORIDE, PROLIFERATION, EPITHELIUM, Junctional proteins, INTERCELLULAR COMMUNICATION, SPERMATOGENIC CELLS, EXPRESSION, EXPOSURE, SYSTEM, [SDV]Life Sciences [q-bio], [INFO]Computer Science [cs]

الوصف: International audience ; Exposure to toxic metals, specifically those belonging to the nonessential group leads to human health defects and among them reprotoxic effects. The mechanisms by which these metals produce their negative effects on spermatogenesis have not been fully elucidated. By using the Durand's validated seminiferous tubule culture model, which mimics the in vivo situation, we recently reported that concentrations of hexavalent chromium, reported in the literature to be closed to that found in the blood circulation of men, increase the number of germ cell cytogenetic abnormalities. Since this metal is also known to affect cellular junctions, we investigated, in the present study, its potential influence on the Sertoli cell barrier and on junctional proteins present at this level such as connexin 43, claudin-11 and N-cadherin. Cultured seminiferous tubules in bicameral chambers expressed the three junctional proteins and ZO-1 for at least 12 days. Exposure to low concentrations of chromium (10 mu g/l) increased the trans-epithelial resistance without major changes of claudin-11 and N-cadherin expressions but strongly delocalized the gap junction protein connexin 43 from the membrane to the cytoplasm of Sertoli cells. The possibility that the hexavalent chromium-induced alteration of connexin 43 indirectly mediates the effect of the toxic metal on the blood testis barrier dynamic is postulated. (c) 2013 Elsevier Inc. All rights reserved.

العلاقة: hal-02651875; https://hal.inrae.fr/hal-02651875Test; PRODINRA: 209151; WOS: 000316305600003

الإتاحة: https://doi.org/10.1016/j.taap.2013.01.016Test
https://hal.inrae.fr/hal-02651875Test

المؤلفون: Carette, Diane, Perrard, Marie-Hélène, Prisant, Nadia, Gilleron, Jérôme, Pointis, Georges, Segretain, Dominique, Durand, Philippe

المساهمون: Université de Nice Sophia-Antipolis (UNSA), UMR S775, Université Paris Descartes - Paris 5 (UPD5), Institut de Génomique Fonctionnelle de Lyon (IGFL), École normale supérieure de Lyon (ENS de Lyon)-Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon, Université de Versailles Saint-Quentin-en-Yvelines (UVSQ), Kallistem SAS, Partenaires INRAE

المصدر: ISSN: 0041-008X.

مصطلحات موضوعية: Hexavalent chromium, Blood-testis barrier, Spermatogenesis, The rat seminiferous tubule culture model, IN-VITRO, CADMIUM CHLORIDE, PROLIFERATION, EPITHELIUM, Junctional proteins, INTERCELLULAR COMMUNICATION, SPERMATOGENIC CELLS, EXPRESSION, EXPOSURE, SYSTEM, [SDV]Life Sciences [q-bio], [INFO]Computer Science [cs]

الوصف: International audience ; Exposure to toxic metals, specifically those belonging to the nonessential group leads to human health defects and among them reprotoxic effects. The mechanisms by which these metals produce their negative effects on spermatogenesis have not been fully elucidated. By using the Durand's validated seminiferous tubule culture model, which mimics the in vivo situation, we recently reported that concentrations of hexavalent chromium, reported in the literature to be closed to that found in the blood circulation of men, increase the number of germ cell cytogenetic abnormalities. Since this metal is also known to affect cellular junctions, we investigated, in the present study, its potential influence on the Sertoli cell barrier and on junctional proteins present at this level such as connexin 43, claudin-11 and N-cadherin. Cultured seminiferous tubules in bicameral chambers expressed the three junctional proteins and ZO-1 for at least 12 days. Exposure to low concentrations of chromium (10 mu g/l) increased the trans-epithelial resistance without major changes of claudin-11 and N-cadherin expressions but strongly delocalized the gap junction protein connexin 43 from the membrane to the cytoplasm of Sertoli cells. The possibility that the hexavalent chromium-induced alteration of connexin 43 indirectly mediates the effect of the toxic metal on the blood testis barrier dynamic is postulated. (c) 2013 Elsevier Inc. All rights reserved.

العلاقة: hal-02651875; https://hal.inrae.fr/hal-02651875Test; PRODINRA: 209151; WOS: 000316305600003

الإتاحة: https://doi.org/10.1016/j.taap.2013.01.016Test
https://hal.inrae.fr/hal-02651875Test

المؤلفون: Carette, Diane, Perrard, Marie-Hélène, Prisant, Nadia, Gilleron, Jérôme, Pointis, Georges, Segretain, Dominique, Durand, Philippe

المساهمون: Université de Nice Sophia-Antipolis (UNSA), UMR S775, Université Paris Descartes - Paris 5 (UPD5), Institut de Génomique Fonctionnelle de Lyon (IGFL), École normale supérieure de Lyon (ENS de Lyon)-Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon, Université de Versailles Saint-Quentin-en-Yvelines (UVSQ), Kallistem SAS, Partenaires INRAE

المصدر: ISSN: 0041-008X.

مصطلحات موضوعية: Hexavalent chromium, Blood-testis barrier, Spermatogenesis, The rat seminiferous tubule culture model, IN-VITRO, CADMIUM CHLORIDE, PROLIFERATION, EPITHELIUM, Junctional proteins, INTERCELLULAR COMMUNICATION, SPERMATOGENIC CELLS, EXPRESSION, EXPOSURE, SYSTEM, [SDV]Life Sciences [q-bio], [INFO]Computer Science [cs]

الوصف: International audience ; Exposure to toxic metals, specifically those belonging to the nonessential group leads to human health defects and among them reprotoxic effects. The mechanisms by which these metals produce their negative effects on spermatogenesis have not been fully elucidated. By using the Durand's validated seminiferous tubule culture model, which mimics the in vivo situation, we recently reported that concentrations of hexavalent chromium, reported in the literature to be closed to that found in the blood circulation of men, increase the number of germ cell cytogenetic abnormalities. Since this metal is also known to affect cellular junctions, we investigated, in the present study, its potential influence on the Sertoli cell barrier and on junctional proteins present at this level such as connexin 43, claudin-11 and N-cadherin. Cultured seminiferous tubules in bicameral chambers expressed the three junctional proteins and ZO-1 for at least 12 days. Exposure to low concentrations of chromium (10 mu g/l) increased the trans-epithelial resistance without major changes of claudin-11 and N-cadherin expressions but strongly delocalized the gap junction protein connexin 43 from the membrane to the cytoplasm of Sertoli cells. The possibility that the hexavalent chromium-induced alteration of connexin 43 indirectly mediates the effect of the toxic metal on the blood testis barrier dynamic is postulated. (c) 2013 Elsevier Inc. All rights reserved.

العلاقة: hal-02651875; https://hal.inrae.fr/hal-02651875Test; PRODINRA: 209151; WOS: 000316305600003

الإتاحة: https://doi.org/10.1016/j.taap.2013.01.016Test
https://hal.inrae.fr/hal-02651875Test

المؤلفون: Carette, Diane, Perrard, Marie-Hélène, Prisant, Nadia, Gilleron, Jérôme, Pointis, Georges, Segretain, Dominique, Durand, Philippe

المساهمون: Université de Nice Sophia-Antipolis (UNSA), UMR S775, Université Paris Descartes - Paris 5 (UPD5), Institut de Génomique Fonctionnelle de Lyon (IGFL), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA)-École normale supérieure - Lyon (ENS Lyon), Centre National de la Recherche Scientifique (CNRS), Université de Lyon, Université de Versailles Saint-Quentin-en-Yvelines (UVSQ), Kallistem SAS, Partenaires INRAE

المصدر: ISSN: 0041-008X.

مصطلحات موضوعية: Hexavalent chromium, Blood-testis barrier, Spermatogenesis, The rat seminiferous tubule culture model, IN-VITRO, CADMIUM CHLORIDE, PROLIFERATION, EPITHELIUM, Junctional proteins, INTERCELLULAR COMMUNICATION, SPERMATOGENIC CELLS, EXPRESSION, EXPOSURE, SYSTEM, [SDV]Life Sciences [q-bio], [INFO]Computer Science [cs]

الوصف: International audience ; Exposure to toxic metals, specifically those belonging to the nonessential group leads to human health defects and among them reprotoxic effects. The mechanisms by which these metals produce their negative effects on spermatogenesis have not been fully elucidated. By using the Durand's validated seminiferous tubule culture model, which mimics the in vivo situation, we recently reported that concentrations of hexavalent chromium, reported in the literature to be closed to that found in the blood circulation of men, increase the number of germ cell cytogenetic abnormalities. Since this metal is also known to affect cellular junctions, we investigated, in the present study, its potential influence on the Sertoli cell barrier and on junctional proteins present at this level such as connexin 43, claudin-11 and N-cadherin. Cultured seminiferous tubules in bicameral chambers expressed the three junctional proteins and ZO-1 for at least 12 days. Exposure to low concentrations of chromium (10 mu g/l) increased the trans-epithelial resistance without major changes of claudin-11 and N-cadherin expressions but strongly delocalized the gap junction protein connexin 43 from the membrane to the cytoplasm of Sertoli cells. The possibility that the hexavalent chromium-induced alteration of connexin 43 indirectly mediates the effect of the toxic metal on the blood testis barrier dynamic is postulated. (c) 2013 Elsevier Inc. All rights reserved.

العلاقة: hal-02651875; https://hal.inrae.fr/hal-02651875Test; PRODINRA: 209151; WOS: 000316305600003

الإتاحة: https://doi.org/10.1016/j.taap.2013.01.016Test
https://hal.inrae.fr/hal-02651875Test

المؤلفون: Geoffroy-Siraudin, Cendrine, Perrard, Marie-Hélène, Chaspoul, Florence, Lanteaume, Andre, Gallice, Philippe, Durand, Philippe, Guichaoua, Marie-Roberte

المساهمون: Biogénotoxicologie et Mutagenèse Environnementale (BME), Université de la Méditerranée - Aix-Marseille 2, Centre National de la Recherche Scientifique (CNRS), Assistance Publique - Hôpitaux de Marseille (APHM), Institut de Génomique Fonctionnelle de Lyon (IGFL), École normale supérieure de Lyon (ENS de Lyon)-Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), CNRS, INRA, Université Claude Bernard Lyon I, Ecole Normale supérieure de Lyon, Université de Méditerranée

المصدر: ISSN: 1096-6080.

مصطلحات موضوعية: spermatogenesis, rat, hexavalent chromium, in-vitro, chromosome, spermatocytes, quality, meiosis, culture, synaptonemal complex, synaptonemal complex damage, spermatogenic cells, sertoli-cells, germ-cells, population, exposure, [SDV]Life Sciences [q-bio], [INFO]Computer Science [cs]

الوصف: International audience ; There is evidence that exposure to environmental factors is at least partly responsible for changes in semen quality observed over the past decades. The detection of reproductive toxicants under Registration, Evaluation and Authorisation of Chemicals (REACH) will impact animal use for regulatory safety testing. We first validated a model of culture of rat seminiferous tubules for toxicological studies on spermatogenesis. Then, using this model of culture, we assessed the deleterious effects of 1, 10, and 100 μg/l hexavalent chromium [Cr(VI)] on meiotic cells. The prophase I of meiosis was studied in vivo and ex vivo. Bromo-2′-deoxyuridine (BrdU) was used to describe the kinetics of germ cell differentiation. SCP3 labeling allowed to establish the distribution of the stages of the meiotic prophase I and to perform a qualitative study of the pachytene stage in the absence or presence of Cr(VI). The development of the meiotic step of pubertal rats was similar in vivo and ex vivo. The number of total cells appeared not affected by the presence of Cr(VI) irrespective of its concentration. However, the numbers of late spermatocytes and of round spermatids were decreased by Cr(VI) even at the lower concentration. The percentage of synaptonemal complex abnormalities increased slightly with the time of culture and dramatically with Cr(VI) concentrations. This model of culture appears suitable for toxicological studies. This study shows that Cr(VI) is toxic for meiotic cells even at low concentrations, and its toxicity increases in a dose-dependent manner.

العلاقة: hal-02665346; https://hal.inrae.fr/hal-02665346Test; PRODINRA: 225899; WOS: 000279316500028

الإتاحة: https://doi.org/10.1093/toxsci/kfq099Test
https://hal.inrae.fr/hal-02665346Test

المؤلفون: Geoffroy-Siraudin, Cendrine, Perrard, Marie-Hélène, Chaspoul, Florence, Lanteaume, Andre, Gallice, Philippe, Durand, Philippe, Guichaoua, Marie-Roberte

المساهمون: Biogénotoxicologie et Mutagenèse Environnementale (BME), Université de la Méditerranée - Aix-Marseille 2, Centre National de la Recherche Scientifique (CNRS), Assistance Publique - Hôpitaux de Marseille (APHM), Institut de Génomique Fonctionnelle de Lyon (IGFL), École normale supérieure de Lyon (ENS de Lyon)-Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), CNRS, INRA, Université Claude Bernard Lyon I, Ecole Normale supérieure de Lyon, Université de Méditerranée

المصدر: ISSN: 1096-6080.

مصطلحات موضوعية: spermatogenesis, rat, hexavalent chromium, in-vitro, chromosome, spermatocytes, quality, meiosis, culture, synaptonemal complex, synaptonemal complex damage, spermatogenic cells, sertoli-cells, germ-cells, population, exposure, [SDV]Life Sciences [q-bio], [INFO]Computer Science [cs]

الوصف: International audience ; There is evidence that exposure to environmental factors is at least partly responsible for changes in semen quality observed over the past decades. The detection of reproductive toxicants under Registration, Evaluation and Authorisation of Chemicals (REACH) will impact animal use for regulatory safety testing. We first validated a model of culture of rat seminiferous tubules for toxicological studies on spermatogenesis. Then, using this model of culture, we assessed the deleterious effects of 1, 10, and 100 μg/l hexavalent chromium [Cr(VI)] on meiotic cells. The prophase I of meiosis was studied in vivo and ex vivo. Bromo-2′-deoxyuridine (BrdU) was used to describe the kinetics of germ cell differentiation. SCP3 labeling allowed to establish the distribution of the stages of the meiotic prophase I and to perform a qualitative study of the pachytene stage in the absence or presence of Cr(VI). The development of the meiotic step of pubertal rats was similar in vivo and ex vivo. The number of total cells appeared not affected by the presence of Cr(VI) irrespective of its concentration. However, the numbers of late spermatocytes and of round spermatids were decreased by Cr(VI) even at the lower concentration. The percentage of synaptonemal complex abnormalities increased slightly with the time of culture and dramatically with Cr(VI) concentrations. This model of culture appears suitable for toxicological studies. This study shows that Cr(VI) is toxic for meiotic cells even at low concentrations, and its toxicity increases in a dose-dependent manner.

العلاقة: hal-02665346; https://hal.inrae.fr/hal-02665346Test; PRODINRA: 225899; WOS: 000279316500028

الإتاحة: https://doi.org/10.1093/toxsci/kfq099Test
https://hal.inrae.fr/hal-02665346Test

المؤلفون: Geoffroy-Siraudin, Cendrine, Perrard, Marie-Hélène, Chaspoul, Florence, Lanteaume, Andre, Gallice, Philippe, Durand, Philippe, Guichaoua, Marie-Roberte

المساهمون: Biogénotoxicologie et Mutagenèse Environnementale (BME), Université de la Méditerranée - Aix-Marseille 2, Centre National de la Recherche Scientifique (CNRS), Assistance Publique - Hôpitaux de Marseille (APHM), Institut de Génomique Fonctionnelle de Lyon (IGFL), École normale supérieure de Lyon (ENS de Lyon)-Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), CNRS, INRA, Université Claude Bernard Lyon I, Ecole Normale supérieure de Lyon, Université de Méditerranée

المصدر: ISSN: 1096-6080.

مصطلحات موضوعية: spermatogenesis, rat, hexavalent chromium, in-vitro, chromosome, spermatocytes, quality, meiosis, culture, synaptonemal complex, synaptonemal complex damage, spermatogenic cells, sertoli-cells, germ-cells, population, exposure, [SDV]Life Sciences [q-bio], [INFO]Computer Science [cs]

الوصف: International audience ; There is evidence that exposure to environmental factors is at least partly responsible for changes in semen quality observed over the past decades. The detection of reproductive toxicants under Registration, Evaluation and Authorisation of Chemicals (REACH) will impact animal use for regulatory safety testing. We first validated a model of culture of rat seminiferous tubules for toxicological studies on spermatogenesis. Then, using this model of culture, we assessed the deleterious effects of 1, 10, and 100 μg/l hexavalent chromium [Cr(VI)] on meiotic cells. The prophase I of meiosis was studied in vivo and ex vivo. Bromo-2′-deoxyuridine (BrdU) was used to describe the kinetics of germ cell differentiation. SCP3 labeling allowed to establish the distribution of the stages of the meiotic prophase I and to perform a qualitative study of the pachytene stage in the absence or presence of Cr(VI). The development of the meiotic step of pubertal rats was similar in vivo and ex vivo. The number of total cells appeared not affected by the presence of Cr(VI) irrespective of its concentration. However, the numbers of late spermatocytes and of round spermatids were decreased by Cr(VI) even at the lower concentration. The percentage of synaptonemal complex abnormalities increased slightly with the time of culture and dramatically with Cr(VI) concentrations. This model of culture appears suitable for toxicological studies. This study shows that Cr(VI) is toxic for meiotic cells even at low concentrations, and its toxicity increases in a dose-dependent manner.

العلاقة: hal-02665346; https://hal.inrae.fr/hal-02665346Test; PRODINRA: 225899; WOS: 000279316500028

الإتاحة: https://doi.org/10.1093/toxsci/kfq099Test
https://hal.inrae.fr/hal-02665346Test