دورية أكاديمية
Antagonistic activities of CDC14B and CDK1 on USP9X regulate WT1-dependent mitotic transcription and survival.
| العنوان: | Antagonistic activities of CDC14B and CDK1 on USP9X regulate WT1-dependent mitotic transcription and survival. |
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| المؤلفون: | Dietachmayr, Michael, Rathakrishnan, Abirami, Gloeckner, Christian Johannes, Clemm von Hohenberg, Katharina, Bassermann, Florian, Karpiuk, Oleksandra, von Zweydorf, Felix, Engleitner, Thomas, Fernández-Sáiz, Vanesa, Schenk, Petra, Ueffing, Marius, Rad, Roland, Eilers, Martin |
| المصدر: | Nature Communications 11(1), 1268 (2020). doi:10.1038/s41467-020-15059-5 |
| بيانات النشر: | Nature Publishing Group UK |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | info:eu-repo/classification/ddc/500, A549 Cells, Apoptosis, CDC2 Protein Kinase: antagonists & inhibitors, Dual-Specificity Phosphatases: antagonists & inhibitors, Gene Knockdown Techniques, HEK293 Cells, HeLa Cells, Humans, Interleukin-8: metabolism, Mitosis: physiology, Phosphorylation, Transcription Factors, Ubiquitin Thiolesterase: drug effects, Ubiquitin Thiolesterase: genetics, Ubiquitin Thiolesterase: metabolism, WT1 Proteins: genetics, WT1 Proteins: metabolism, CXCL8 protein, human, Interleukin-8, USP9X protein, WT1 Proteins, WT1 protein, CDC2 Protein Kinase, CDK1 protein, CDC14B protein, Dual-Specificity Phosphatases, Ubiquitin Thiolesterase |
| جغرافية الموضوع: | DE |
| الوصف: | Regulation of mitosis secures cellular integrity and its failure critically contributes to the development, maintenance, and treatment resistance of cancer. In yeast, the dual phosphatase Cdc14 controls mitotic progression by antagonizing Cdk1-mediated protein phosphorylation. By contrast, specific mitotic functions of the mammalian Cdc14 orthologue CDC14B have remained largely elusive. Here, we find that CDC14B antagonizes CDK1-mediated activating mitotic phosphorylation of the deubiquitinase USP9X at serine residue 2563, which we show to be essential for USP9X to mediate mitotic survival. Starting from an unbiased proteome-wide screening approach, we specify Wilms' tumor protein 1 (WT1) as the relevant substrate that becomes deubiquitylated and stabilized by serine 2563-phosphorylated USP9X in mitosis. We further demonstrate that WT1 functions as a mitotic transcription factor and specify CXCL8/IL-8 as a target gene of WT1 that conveys mitotic survival. Together, we describe a ubiquitin-dependent signaling pathway that directs a mitosis-specific transcription program to regulate mitotic survival. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/issn/2041-1723; info:eu-repo/semantics/altIdentifier/pmid/pmid:32152317; https://pub.dzne.de/record/153412Test; https://pub.dzne.de/search?p=id:%22DZNE-2020-01409%22Test |
| الإتاحة: | https://doi.org/10.1038/s41467-020-15059-5Test https://pub.dzne.de/record/153412Test https://pub.dzne.de/search?p=id:%22DZNE-2020-01409%22Test |
| حقوق: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.2089D15D |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |