دورية أكاديمية
A Biochemical Corrosion Monitoring Sensor with a Silver/Carbon Comb Structure for the Detection of Living Escherichia coli
| العنوان: | A Biochemical Corrosion Monitoring Sensor with a Silver/Carbon Comb Structure for the Detection of Living Escherichia coli |
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| المؤلفون: | Chiyako Touge, Michiyo Nakatsu, Mai Sugimoto, Eiichiro Takamura, Hiroaki Sakamoto |
| سنة النشر: | 2023 |
| مصطلحات موضوعية: | Biophysics, Biochemistry, Medicine, Microbiology, Molecular Biology, Biotechnology, Infectious Diseases, Space Science, Environmental Sciences not elsewhere classified, Biological Sciences not elsewhere classified, Chemical Sciences not elsewhere classified, reach 20 na, lb liquid medium, carbon comb structure, carbon comb electrodes, 1 – 10, novel biochemical sensor, living escherichia coli, biochemical corrosion monitoring, bacterial count range, 20 , 2 , c sensor increased, measures galvanic current, coli solution increases, 8 , bacterial solution, c sensor, coli count, galvanic current |
| الوصف: | For the detection and monitoring of live bacteria, we propose a biochemical corrosion monitoring (BCM) sensor that measures galvanic current by using a Ag/C sensor comprising silver and carbon comb electrodes. The deposition of an Escherichia coli suspension containing an LB liquid medium on the Ag/C sensor increased the galvanic current. The time required for the current to reach 20 nA is defined as T 20 . T 20 tends to decrease as the initial number of E. coli in the E. coli solution increases. A linear relationship was obtained between the logarithm of the E. coli count and T 20 in a bacterial count range of 1–10 8 cfu/mL under culture conditions in which the growth rate of the bacteria was constant. Hence, the number of live E. coli could be determined from T 20 . Ag 2 S precipitation was observed on the surface of the Ag electrode of the Ag/C sensor, where an increase in the current was observed. This generation of galvanic current was attributed to the reaction between a small amount of free H 2 S metabolized by E. coli in the bacterial solution during its growth process and Agthe sensor anode. The Ag/C sensor can detect a free H 2 S concentration of 0.041 μM in the E. coli solution. This novel biochemical sensor can monitor the growth behavior of living organisms without damaging them. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | unknown |
| العلاقة: | https://figshare.com/articles/journal_contribution/A_Biochemical_Corrosion_Monitoring_Sensor_with_a_Silver_Carbon_Comb_Structure_for_the_Detection_of_Living_Escherichia_coli/24550828Test |
| DOI: | 10.1021/acsomega.3c03632.s001 |
| الإتاحة: | https://doi.org/10.1021/acsomega.3c03632.s001Test https://figshare.com/articles/journal_contribution/A_Biochemical_Corrosion_Monitoring_Sensor_with_a_Silver_Carbon_Comb_Structure_for_the_Detection_of_Living_Escherichia_coli/24550828Test |
| حقوق: | CC BY-NC 4.0 |
| رقم الانضمام: | edsbas.D659203F |
| قاعدة البيانات: | BASE |
| DOI: | 10.1021/acsomega.3c03632.s001 |
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