دورية أكاديمية
Spatial proteomics defines the content of trafficking vesicles captured by golgin tethers.
| العنوان: | Spatial proteomics defines the content of trafficking vesicles captured by golgin tethers. |
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| المؤلفون: | Shin, John JH, Crook, Oliver M, Borgeaud, Alicia C, Cattin-Ortolá, Jérôme, Peak-Chew, Sew Y, Breckels, Lisa M, Gillingham, Alison K, Chadwick, Jessica, Lilley, Kathryn S, Munro, Sean |
| بيانات النشر: | Springer Science and Business Media LLC //dx.doi.org/10.1038/s41467-020-19840-4 Nat Commun |
| سنة النشر: | 2020 |
| المجموعة: | Apollo - University of Cambridge Repository |
| مصطلحات موضوعية: | Autoantigens, Endosomes, Gene Knockdown Techniques, Golgi Matrix Proteins, HEK293 Cells, HeLa Cells, Humans, Membrane Proteins, Mitochondria, Proteomics, Spatial Analysis, trans-Golgi Network |
| الوصف: | Intracellular traffic between compartments of the secretory and endocytic pathways is mediated by vesicle-based carriers. The proteomes of carriers destined for many organelles are ill-defined because the vesicular intermediates are transient, low-abundance and difficult to purify. Here, we combine vesicle relocalisation with organelle proteomics and Bayesian analysis to define the content of different endosome-derived vesicles destined for the trans-Golgi network (TGN). The golgin coiled-coil proteins golgin-97 and GCC88, shown previously to capture endosome-derived vesicles at the TGN, were individually relocalised to mitochondria and the content of the subsequently re-routed vesicles was determined by organelle proteomics. Our findings reveal 45 integral and 51 peripheral membrane proteins re-routed by golgin-97, evidence for a distinct class of vesicles shared by golgin-97 and GCC88, and various cargoes specific to individual golgins. These results illustrate a general strategy for analysing intracellular sub-proteomes by combining acute cellular re-wiring with high-resolution spatial proteomics. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | Electronic; application/pdf |
| اللغة: | English |
| العلاقة: | https://www.repository.cam.ac.uk/handle/1810/314784Test |
| DOI: | 10.17863/CAM.61890 |
| الإتاحة: | https://doi.org/10.17863/CAM.61890Test https://www.repository.cam.ac.uk/handle/1810/314784Test |
| حقوق: | Attribution 4.0 International ; https://creativecommons.org/licenses/by/4.0Test/ |
| رقم الانضمام: | edsbas.A3C05F1A |
| قاعدة البيانات: | BASE |
| DOI: | 10.17863/CAM.61890 |
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