دورية أكاديمية
Retromer has a selective function in cargo sorting via endosome transport carriers
| العنوان: | Retromer has a selective function in cargo sorting via endosome transport carriers |
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| المؤلفون: | Cui, Y., Carosi, J.M., Yang, Z., Ariotti, N., Kerr, M.C., Parton, R.G., Sargeant, T.J., Teasdale, R.D. |
| المصدر: | http://dx.doi.org/10.1083/jcb.201806153Test. |
| بيانات النشر: | Rockefeller University Press |
| سنة النشر: | 2019 |
| المجموعة: | The University of Adelaide: Digital Library |
| مصطلحات موضوعية: | Hela Cells, Endosomes, Lysosomes, Humans, Multiprotein Complexes, Receptor, IGF Type 2, Autoantigens, Biological Transport, Active, Sorting Nexins, Golgi Matrix Proteins |
| الوصف: | Retromer is a peripheral membrane protein complex that coordinates multiple vesicular trafficking events within the endolysosomal system. Here, we demonstrate that retromer is required for the maintenance of normal lysosomal morphology and function. The knockout of retromer subunit Vps35 causes an ultrastructural alteration in lysosomal structure and aberrant lysosome function, leading to impaired autophagy. At the whole-cell level, knockout of retromer Vps35 subunit reduces lysosomal proteolytic capacity as a consequence of the improper processing of lysosomal hydrolases, which is dependent on the trafficking of the cation-independent mannose 6-phosphate receptor (CI-M6PR). Incorporation of CI-M6PR into endosome transport carriers via a retromer-dependent process is restricted to those tethered by GCC88 but not golgin-97 or golgin-245. Finally, we show that this retromer-dependent retrograde cargo trafficking pathway requires SNX3, but not other retromer-associated cargo binding proteins, such as SNX27 or SNX-BAR proteins. Therefore, retromer does contribute to the retrograde trafficking of CI-M6PR required for maturation of lysosomal hydrolases and lysosomal function. ; Yi Cui, Julian M. Carosi, Zhe Yang, Nicholas Ariotti, Markus C. Kerr, Robert G. Parton, Timothy J. Sargeant, and Rohan D. Teasdale |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 0021-9525 1540-8140 |
| العلاقة: | http://purl.org/au-research/grants/arc/DP160101573Test; http://purl.org/au-research/grants/nhmrc/1037320Test; http://purl.org/au-research/grants/nhmrc/1058565Test; http://purl.org/au-research/grants/nhmrc/569542Test; http://purl.org/au-research/grants/nhmrc/1045092Test; http://purl.org/au-research/grants/nhmrc/1041929Test; The Journal of Cell Biology, 2019; 218(2):615-631; http://hdl.handle.net/2440/121043Test; Carosi, J.M. [0000-0001-6820-3953]; Sargeant, T.J. [0000-0003-1254-4390] |
| DOI: | 10.1083/jcb.201806153 |
| الإتاحة: | https://doi.org/10.1083/jcb.201806153Test http://hdl.handle.net/2440/121043Test |
| حقوق: | © 2019 Cui et al. http://www.rupress.org/terms/https://creativecommons.org/licenses/by-nc-sa/4.0/ThisTest article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/termsTest/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0Test/). |
| رقم الانضمام: | edsbas.5250B6DB |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 00219525 15408140 |
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| DOI: | 10.1083/jcb.201806153 |