دورية أكاديمية
Diagnosis and Surveillance of Neonatal Infections by Metagenomic Next-Generation Sequencing
العنوان: | Diagnosis and Surveillance of Neonatal Infections by Metagenomic Next-Generation Sequencing |
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المؤلفون: | Rong Zhang, Yan Zhuang, Zheng-hui Xiao, Cai-yun Li, Fan Zhang, Wei-qing Huang, Min Zhang, Xiao-Ming Peng, Chao Liu |
المصدر: | Frontiers in Microbiology, Vol 13 (2022) |
بيانات النشر: | Frontiers Media S.A. |
سنة النشر: | 2022 |
المجموعة: | Directory of Open Access Journals: DOAJ Articles |
مصطلحات موضوعية: | neonatal infections, metagenomic next-generation sequencing, Mycobacterium tuberculosis, Legionella pneumophila, Bacillus cereus, Microbiology, QR1-502 |
الوصف: | Microbial infections cause significant morbidity and mortality in neonates. Metagenomic next-generation sequencing is a hypothesis-free and culture-free test that enables broad identification of pathogens and antimicrobial resistance genes directly from clinical samples within 24 h. In this study, we used mNGS for etiological diagnosis and monitoring the efficacy of antibiotic treatment in a cohort of neonatal patients with severe infections. The median age was 19.5 (3–52) days, median gestational age was 37.96 (31–40+3) weeks, and the median birth weight was 3,261 (1,300–4,300) g. The types of infectious diseases included pneumonia, sepsis, and meningitis. mNGS reported microbial findings in all cases, which led to changes in antibiotic treatment. These included cases of Mycobacterium tuberculosis, Legionella pneumophila, and Bacillus cereus. Eight of ten infants recovered after antibiotic adjustment and showed normal development during follow-up. On the other hand, neurological retardation was seen in two infants with meningitis. mNGS enabled etiological diagnosis and guided antibiotic therapy when all conventional methods failed to discover the culprit. It has the potential to cut down the overall cost and burden of disease management in neonatal infections. |
نوع الوثيقة: | article in journal/newspaper |
اللغة: | English |
تدمد: | 1664-302X |
العلاقة: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.855988/fullTest; https://doaj.org/toc/1664-302XTest; https://doaj.org/article/22e98bb2ea4c4a9cb233295bcf43d945Test |
DOI: | 10.3389/fmicb.2022.855988 |
الإتاحة: | https://doi.org/10.3389/fmicb.2022.855988Test https://doaj.org/article/22e98bb2ea4c4a9cb233295bcf43d945Test |
رقم الانضمام: | edsbas.2FCE8BB8 |
قاعدة البيانات: | BASE |
تدمد: | 1664302X |
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DOI: | 10.3389/fmicb.2022.855988 |