رسالة جامعية
Participação de receptores muscarínicos e da via do óxido nítrico no efeito espasmolítico da fração de alcalóides totais obtida de Solanum paludosum Moric: (Solanaceae) ; Participation of the muscarinic receptors and of the nitric oxide pathway in the spasmolytic effect of the total alkaloids fraction obtained from Solanum paludosum Moric. (SOLANACEAE)
| العنوان: | Participação de receptores muscarínicos e da via do óxido nítrico no efeito espasmolítico da fração de alcalóides totais obtida de Solanum paludosum Moric: (Solanaceae) ; Participation of the muscarinic receptors and of the nitric oxide pathway in the spasmolytic effect of the total alkaloids fraction obtained from Solanum paludosum Moric. (SOLANACEAE) |
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| المؤلفون: | Monteiro, Fábio de Souza |
| المساهمون: | Silva, Bagnolia Araujo da, http://lattes.cnpq.br/2569484428391315Test |
| بيانات النشر: | Universidade Federal da Paraíba BR Farmacologia Programa de Pós-Graduação em Produtos Naturais e Sintéticos Bioativos UFPB |
| سنة النشر: | 2010 |
| المجموعة: | Universidade Federal da Paraiba: Biblioteca Digital de Teses e Dissertações |
| مصطلحات موضوعية: | Solanum paludosum, Atividades citotóxica e espasmolítica, Receptores muscarínicos, Via do óxido nítrico, Cytotoxic activity, Spasmolytic effect, Muscarinic receptor, Nitric oxid pathway, CIENCIAS BIOLOGICAS::FARMACOLOGIA |
| الوصف: | Solanum paludosum Moric. (Solanaceae) is an herbaceous species, known popularly as "jurubeba-roxa" in the Northeast of Brazil (AGRA; BHATTACHARYYA, 1999). The chemical and pharmacological studies with root bark of this species showed the presence of glycoalkaloids, and that the ethanol extract and aqueous phase obtained the same parts showed spasmolytic activity (ATAÍDE, 1982; BARBOSA-FILHO et al., 1991; BASÍLIO, 2008). As glycoalkaloids are known to present cytotoxicity and many species of Solanum present spasmolytic activity, decided to study the total alkaloids fraction from root bark of S. paludosum (FAT-SP) on the cytotoxic potential in rat s erythrocytes and to investigate the spasmolytic activity in rat uterus, rat aorta and guinea-pig ileum and trachea. FAT-SP did not show hemolytic activity but presented non-selective spasmolytic effect. The preliminary pharmacological screening showed that FAT-SP on rat uterus, did not inhibit the oxytocin (10-2 IU/mL)-induced phasic contractions, but significantly inhibited the CCh (10-5 M)-induced phasic contractions (IC50 = 178.8 ± 7.1 μg/mL). On guinea-pig trachea, was about 2.2 times more potent in relax the trachea pre-contracted with CCh 10-6 M in the absence (EC50 = 159.4 ± 23.0 μg/mL) than in the presence (EC50 = 353.2 ±15.2 μg/mL) of functional epithelium; on guinea-pig ileum, inhibited the CCh (10-6 M)-induced phasic contractions (IC50 = 129.8 ± 3.7 μg/mL) and was more potent in relation to histamine (10-6 M)-induced contractions (Emax = 18.4 ± 2.3%) and on rat aorta, we observed that FAT-SP relaxes the rat aorta pre-contracted with phenylephrine 3 x 10-7 M of manner more potent in the presence (EC50 = 75.4 ± 6.2 μg/mL) than absence (EC50 = 242.8 ± 11.7 g/mL) of functional endothelium. According with the parameter of potency FAT-SP was more potent on guinea-pig ileum and rat aorta. Thus, we decided to investigate a possible mechanism of action in these organs. FAT-SP relaxed in a significant and concentration dependent manner the guinea-pig ileum ... |
| نوع الوثيقة: | thesis |
| وصف الملف: | application/pdf |
| اللغة: | Portuguese |
| العلاقة: | https://repositorio.ufpb.br/jspui/handle/tede/6832Test |
| الإتاحة: | https://repositorio.ufpb.br/jspui/handle/tede/6832Test |
| حقوق: | Acesso Aberto |
| رقم الانضمام: | edsbas.42937DB7 |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |