دورية أكاديمية
Embryonic stem cell-derived hemangioblasts remain epigenetically plastic and require PRC1 to prevent neural gene expression.
| العنوان: | Embryonic stem cell-derived hemangioblasts remain epigenetically plastic and require PRC1 to prevent neural gene expression. |
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| المؤلفون: | Mazzarella, Luca, Jørgensen, Helle F, Soza-Ried, Jorge, Terry, Anna V, Pearson, Stella, Lacaud, Georges, Kouskoff, Valerie, Merkenschlager, Matthias, Fisher, Amanda G |
| بيانات النشر: | American Society of Hematology //dx.doi.org/10.1182/blood-2010-03-273128 Blood |
| سنة النشر: | 2011 |
| المجموعة: | Apollo - University of Cambridge Repository |
| مصطلحات موضوعية: | Animals, Blotting, Western, Cell Differentiation, Cells, Cultured, Chromatin, Chromatin Immunoprecipitation, DNA-Binding Proteins, Embryonic Stem Cells, Epigenesis, Genetic, Fetal Proteins, Green Fluorescent Proteins, Hemangioblasts, Histones, Homeobox Protein Nkx-2.2, Homeodomain Proteins, Integrases, Mice, Knockout, Polycomb Repressive Complex 1, Polycomb-Group Proteins, RNA, Messenger, Repressor Proteins, Reverse Transcriptase Polymerase Chain Reaction, SOXB1 Transcription Factors, T-Box Domain Proteins, Transcription Factors |
| الوصف: | Many lineage-specific developmental regulator genes are transcriptionally primed in embryonic stem (ES) cells; RNA Pol(II) is bound at their promoters but is prevented from productive elongation by the activity of polycomb repressive complexes (PRC) 1 and 2. This epigenetically poised state is thought to enable ES cells to rapidly execute multiple differentiation programs and is recognized by a simultaneous enrichment for trimethylation of lysine 4 and trimethylation of lysine 27 of histone H3 (bivalent chromatin) across promoter regions. Here we show that the chromatin profile of this important cohort of genes is progressively modified as ES cells differentiate toward blood-forming precursors. Surprisingly however, neural specifying genes, such as Nkx2-2, Nkx2-9, and Sox1, remain bivalent and primed even in committed hemangioblasts, as conditional deletion of PRC1 results in overt and inappropriate expression of neural genes in hemangioblasts. These data reinforce the importance of PRC1 for normal hematopoietic differentiation and reveal an unexpected epigenetic plasticity of mesoderm-committed hemangioblasts. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | Print-Electronic; application/pdf |
| اللغة: | English |
| العلاقة: | https://www.repository.cam.ac.uk/handle/1810/289289Test |
| DOI: | 10.17863/CAM.22746 |
| الإتاحة: | https://doi.org/10.17863/CAM.22746Test https://www.repository.cam.ac.uk/handle/1810/289289Test |
| رقم الانضمام: | edsbas.EF9C8845 |
| قاعدة البيانات: | BASE |
| DOI: | 10.17863/CAM.22746 |
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