Images showing the microscope setup for ToC cultures.

التفاصيل البيبلوغرافية
العنوان: Images showing the microscope setup for ToC cultures.
المؤلفون: Irina Veith, Arianna Mencattini, Valentin Picant, Marco Serra, Marine Leclerc, Maria Colomba Comes, Fathia Mami-Chouaib, Jacques Camonis, Stéphanie Descroix, Hamasseh Shirvani, Fatima Mechta-Grigoriou, Gérard Zalcman, Maria Carla Parrini, Eugenio Martinelli
سنة النشر: 2021
مصطلحات موضوعية: Biophysics, Cell Biology, Genetics, Pharmacology, Biotechnology, Immunology, Cancer, Hematology, Biological Sciences not elsewhere classified, Chemical Sciences not elsewhere classified, Information Systems not elsewhere classified, cancer cells, 3 D tumor ecosystems, cell co-culture videos, patio t emporal, doxorubicin chemotherapy drug, CTL, apoptotic cell death, cytotoxic cancer death, breast cancer chemo-resistance, biomimetic 3 D hydrogels, novel video analysis algorithms, drug resistance mechanisms, autologous tumor-infiltrating cytot, CAF, cancer death, cell culture techniques, socio, art
الوصف: A. Global view of the Leica DMi8 used to perform the live imaging experiments. The white arrow points at the heating black chamber, in which is maintained a temperature of 37°C. The temperature is set and maintained by a temperature controller (blue arrow). The CO 2 controller (black arrow) mixes CO 2 with atmospheric air. Through a tubing system, the air with the controlled CO 2 at 5%, after passing through a bottle half filled with water for humidification (red arrow), is injected in the microscope chamber. B. View of the chip placed in the microscope chamber. The white arrow indicates the lid of the chamber in which is injected the humidified air with CO 2 at 5%. The red arrow points at the chip to be imaged. Humidified sponges contribute to humidify the chamber and to minimize micro-evaporation phenomena (black arrows). C. Picture from the side of the chip filled with medium. At each ‘anchor’ point, three magnets (red arrows) are used to lift and attach the chip to the glass slide (black arrow). D. Top view of an empty chip with magnets. Two piles composed of three magnets (red arrows) are applied in the central part of the chip and glass slide. (PNG)
نوع الوثيقة: still image
اللغة: unknown
العلاقة: https://doi.org/10.1371/journal.pcbi.1008870.s005Test
DOI: 10.1371/journal.pcbi.1008870.s005
الإتاحة: https://doi.org/10.1371/journal.pcbi.1008870.s005Test
حقوق: undefined
رقم الانضمام: edsbas.3A221F10
قاعدة البيانات: BASE