دورية أكاديمية
Repeat-Unit Elongations To Produce Bacterial Complex Long Polysaccharide Chains, an O-Antigen Perspective.
| العنوان: | Repeat-Unit Elongations To Produce Bacterial Complex Long Polysaccharide Chains, an O-Antigen Perspective. |
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| المؤلفون: | Hong, Yaoqin, Hu, Dalong, Verderosa, Anthony D, Qin, Jilong, Totsika, Makrina, Reeves, Peter R |
| المصدر: | EcoSal Plus ; ISSN:2324-6200 ; Volume:11 ; Issue:1 |
| بيانات النشر: | Atypon |
| سنة النشر: | 2023 |
| المجموعة: | PubMed Central (PMC) |
| مصطلحات موضوعية: | O-antigen, Wzx, Wzy polymerase, Wzy-dependent pathway, Wzz, capsule, cell envelope, lipopolysaccharide, polysaccharide elongation |
| الوصف: | The O-antigen, a long polysaccharide that constitutes the distal part of the outer membrane-anchored lipopolysaccharide, is one of the critical components in the protective outer membrane of Gram-negative bacteria. Most species produce one of the structurally diverse O-antigens, with nearly all the polysaccharide components having complex structures made by the Wzx/Wzy pathway. This pathway produces repeat-units of mostly 3-8 sugars on the cytosolic face of the cytoplasmic membrane that is translocated by Wzx flippase to the periplasmic face and polymerized by Wzy polymerase to give long-chain polysaccharides. The Wzy polymerase is a highly diverse integral membrane protein typically containing 10-14 transmembrane segments. Biochemical evidence confirmed that Wzy polymerase is the sole driver of polymerization, and recent progress also began to demystify its interacting partner, Wzz, shedding some light to speculate how the proteins may operate together during polysaccharide biogenesis. However, our knowledge of how the highly variable Wzy proteins work as part of the O-antigen processing machinery remains poor. Here, we discuss the progress to the current understanding of repeat-unit polymerization and propose an updated model to explain the formation of additional short chain O-antigen polymers found in the lipopolysaccharide of diverse Gram-negative species and their importance in the biosynthetic process. |
| نوع الوثيقة: | article in journal/newspaper review |
| اللغة: | English |
| العلاقة: | https://doi.org/10.1128/ecosalplus.esp-0020-2022Test; https://pubmed.ncbi.nlm.nih.gov/36622162Test; https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10729934Test/ |
| DOI: | 10.1128/ecosalplus.esp-0020-2022 |
| الإتاحة: | https://doi.org/10.1128/ecosalplus.esp-0020-2022Test https://pubmed.ncbi.nlm.nih.gov/36622162Test https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10729934Test/ |
| رقم الانضمام: | edsbas.13488BB6 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1128/ecosalplus.esp-0020-2022 |
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