التفاصيل البيبلوغرافية
| العنوان: |
Solid-phase extraction for purification of alkannin/shikonin samples and isolation of monomeric and dimeric fractions. |
| المؤلفون: |
Noula, E.1, Samanidou, V. F.1, Assimopoulou, A. N.2 adreana@eng.auth.gr, Papageorgiou, V. P.2, Papadoyannis, I. N.1 papadoya@chem.auth.gr |
| المصدر: |
Analytical & Bioanalytical Chemistry. Jul2010, Vol. 397 Issue 6, p2221-2232. 12p. 1 Color Photograph, 3 Diagrams, 6 Charts, 3 Graphs. |
| مصطلحات موضوعية: |
*SOLID-phase synthesis, *NAPHTHOQUINONE, *NAPHTHALENE, *QUINONE, *ANALYTICAL chemistry |
| مستخلص: |
Isohexenylnaphthazarins (IHN), commonly known as alkannins and shikonins (A/S), are potent pharmaceutical substances with a wide spectrum of wound healing, antimicrobial, anti-inflammatory, and antitumor activity. Purification of A/S is crucial for their use in pharmaceuticals and for biological experimentation. Dimeric and oligomeric A/S derivatives co-exist with the active monomeric ones in most of the samples produced either by (semi)-synthesis or biotechnologically or isolated from natural products. Oligomeric A/S derivatives have not been studied for biological activity hitherto and a method to isolate them is essential. In the present study, solid-phase extraction (SPE) was applied for purification of commercial samples and isolation of monomeric and oligomeric A/S fractions, testing several stationary phases. Sephadex LH-20 cartridges achieved efficient purification for commercial samples containing both monomeric and dimeric A/S derivatives and also separation and isolation of both pure monomeric and dimeric A/S fractions for biological experiments. A high-performance liquid chromatography-diode array detection method was applied for detection, identification and quantification of monomeric and oligomeric shikonin fractions. [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: |
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