التفاصيل البيبلوغرافية
| العنوان: |
Multiplex immunohistochemistry accurately defines the immune context of metastatic melanoma. |
| المؤلفون: |
Halse, H.1 (AUTHOR), Colebatch, A. J.2 (AUTHOR), Petrone, P.1 (AUTHOR), Henderson, M. A.1 (AUTHOR), Mills, J. K.1,3 (AUTHOR), Snow, H.3 (AUTHOR), Westwood, J. A.1 (AUTHOR), Sandhu, S.2,4 (AUTHOR), Raleigh, J. M.2 (AUTHOR), Behren, A.5,6 (AUTHOR), Cebon, J.5,6 (AUTHOR), Darcy, P. K.1,4 (AUTHOR), Kershaw, M. H.1,4 (AUTHOR), McArthur, G. A.2 (AUTHOR), Gyorki, D. E.1,3,7 (AUTHOR), Neeson, P. J.1,4 (AUTHOR) paul.neeson@petermac.org |
| المصدر: |
Scientific Reports. 7/24/2018, Vol. 8 Issue 1, p1-9. 9p. |
| مستخلص: |
A prospective study explored the heterogeneous nature of metastatic melanoma using Multiplex immunohistochemistry (IHC) and flow cytometry (FACS). Multiplex IHC data quantitated immune subset number present intra-tumoral (IT) vs the tumor stroma, plus distance of immune subsets from the tumor margin (TM). In addition, mIHC showed a close association between the presence of IT CD8+ T cells and PDL1 expression in melanoma, which was more prevalent on macrophages than on melanoma cells. In contrast, FACS provided more detailed information regarding the T cell subset differentiation, their activation status and expression of immune checkpoint molecules. Interestingly, mIHC detected significantly higher Treg numbers than FACS and showed preferential CD4+ T cell distribution in the tumor stroma. Based on the mIHC and FACS data, we provide a model which defines metastatic melanoma immune context into four categories using the presence or absence of PDL1+ melanoma cells and/or macrophages, and their location within the tumor or on the periphery, combined with the presence or absence of IT CD8+ T cells. This model interprets melanoma immune context as a spectrum of tumor escape from immune control, and provides a snapshot upon which interpretation of checkpoint blockade inhibitor (CBI) therapy responses can be built. [ABSTRACT FROM AUTHOR] |
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