التفاصيل البيبلوغرافية
| العنوان: |
Coinfection with Human Norovirus and Escherichia coli O25:H4 Harboring Two Chromosomal blaCTX-M-14 Genes in a Foodborne Norovirus Outbreak in Shizuoka Prefecture, Japan. |
| المؤلفون: |
HIROMI NAGAOKA1 (AUTHOR), SHINICHIRO HIRAI2 (AUTHOR) hirai@nih.go.jp, HIROTAKA MORINUSHI1 (AUTHOR), SHIRO MIZUMOTO1 (AUTHOR), KANA SUZUKI1 (AUTHOR), HIROAKI SHIGEMURA3 (AUTHOR), NAOTO TAKAHASHI4 (AUTHOR), FUMIE SUZUKI4 (AUTHOR), MIZUHA MOCHIZUKI4 (AUTHOR), MICHIKO ASANUMA4 (AUTHOR), TAKAHARU MAEHATA4 (AUTHOR), AYA OGAWA1 (AUTHOR), KAI OHKOSHI1 (AUTHOR), TSUYOSHI SEKIZUKA5 (AUTHOR), TAISEI ISHIOKA6 (AUTHOR), SATOWA SUZUKI7 (AUTHOR), HIROKAZU KIMURA8 (AUTHOR), MAKOTO KURODA5 (AUTHOR), MOTOI SUZUKI2 (AUTHOR), KOICHI MURAKAMI2 (AUTHOR) |
| المصدر: |
Journal of Food Protection. Sep2020, Vol. 83 Issue 9, p1584-1591. 8p. 2 Diagrams, 3 Charts, 1 Graph. |
| مستخلص: |
Hospital-acquired infections caused by extended-spectrum β-lactamase (ESBL)–producing Escherichia coli are a global problem. Healthy people can carry ESBL-producing E. coli in the intestines; thus, E. coli from healthy people can potentially cause hospital-acquired infections. Therefore, the transmission routes of ESBL-producing E. coli from healthy persons should be determined. A foodborne outbreak of human norovirus (HuNoV) GII occurred at a restaurant in Shizuoka, Japan, in 2018. E. coli O25:H4 was isolated from some of the HuNoV-infected customers. Pulsed-field gel electrophoresis showed that these E. coli O25:H4 strains originated from one clone. Because the only epidemiological link among the customers was eating food from this restaurant, the customers were concurrently infected with E. coli O25:H4 and HuNoV GII via the restaurant food. Whole genome analysis revealed that the E. coli O25:H4 strains possessed genes for regulating intracellular iron and expressing the flagellum and flagella. Extraintestinal pathogenic E. coli often express these genes on the chromosome. Additionally, the E. coli O25:H4 strains had plasmids harboring nine antimicrobial resistance genes. These strains harbored ESBL-encoding blaCTX-M-14 genes on two loci of the chromosome and had higher ESBL activity. Multilocus sequence typing and fimH subtyping revealed that the E. coli O25:H4 strains from the outbreak belonged to the subclonal group, ST131-fimH30R, which has been driving ESBL epidemics in Japan. Because the E. coli O25:H4 strains isolated in the outbreak belonged to a subclonal group spreading in Japan, foods contaminated with ESBL-producing E. coli might contribute to spreading these strains among healthy persons. The isolated E. coli O25:H4 strains produced ESBL and contained plasmids with multiple antimicrobial resistance genes, which may make it difficult to select antimicrobials for treating extraintestinal infections caused by these strains. [ABSTRACT FROM AUTHOR] |
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