التفاصيل البيبلوغرافية
| العنوان: |
Macrophage Inhibition Factor-Mediated CD74 Signal Modulate Inflammation and Matrix Metabolism in the Degenerated Cartilage Endplate Chondrocytes by Activating Extracellular Signal Regulated Kinase 1/2. |
| المؤلفون: |
Chengjie Xiong1, Yong Huang1, Hui Kang1, Tonghui Zhang1, Feng Xu1 gkxf79390@sohu.com, Xianhua Cai1 xianhua_cai@126.com, Xiong, Chengjie1 (AUTHOR), Huang, Yong (AUTHOR), Kang, Hui (AUTHOR), Zhang, Tonghui (AUTHOR), Xu, Feng (AUTHOR), Cai, Xianhua (AUTHOR) |
| المصدر: |
Spine (03622436). 1/15/2017, Vol. 42 Issue 2, pE61-E70. 10p. |
| مصطلحات موضوعية: |
*MACROPHAGE migration inhibitory factor, *CARTILAGE cells, *EXTRACELLULAR signal-regulated kinases, *CYTOKINES, *NUCLEUS pulposus, *ANTIGENS, *CELLULAR signal transduction, *FLAVONOIDS, *GENE expression, *HISTOCOMPATIBILITY antigens, *INFLAMMATION, *LYMPHOKINES, *MACROPHAGES, *SPINE diseases, *PROTEIN kinase inhibitors, *PHARMACODYNAMICS |
| مستخلص: |
Study Design: The macrophage inhibition factor (MIF)-mediated CD74 dependent extracellular signal-regulated kinase 1/2 (ERK1/2) phosphorylation were associated with inflammatory activity and matrix metabolism in human degenerated cartilage endplate (CEP). Anabolic/catabolic factors in pathogenesis of CEP degeneration were evaluated.Objective: To study the effect of MIF-mediated CD74 dependent ERK1/2 activation on the CEP degeneration.Summary Of Background Data: MIF-CD74 signal is closely related to the CEP degeneration by inducing the secretion of inflammatory cytokines. ERK1/2-mediated inflammatory pathway also plays a crucial role in the intervertebral disc degeneration. The role of the ERK1/2 pathway in CEP chondrocytes response to MIF-CD74 signal has, however, not been fully elucidated.Methods: Chondrocytes were exposed to MIF, with or without ERK1/2 inhibition; CD74 interfered chondrocytes were also exposed to MIF, with or without ERK inhibition. mRNAs were isolated for real-time polymerase chain reaction measurement of gene expression. Western blotting was carried out to analyze the protein expression.Results: ERK1/2 expression was significantly increased by MIF. MIF modulates metabolism in CEP chondrocytes and decreased by its inhibitor PD98059. ERK1/2 expression was significantly decreased by CD74siRNA. Inflammatory cytokines expression was significantly increased by MIF-induced ERK1/2 activation and significantly suppressed by PD98059. On the contrary, matrix expression was significantly decreased by MIF-induced ERK1/2 activation and reversed by PD98059. CD74siRNA decreased the CD74 expression in chondrocytes. Inflammatory cytokines and matrix expression were not induced by MIF in CD74 interfered chondrocytes.Conclusion: These results show that MIF-CD74 signal elicits an imbalance between anabolic and catabolic metabolism in CEP chondrocytes via ERK signal pathway. ERK inhibition could exert therapeutic effect against the harmful effects of MIF-CD74 signal in CEP degeneration.Level Of Evidence: N/A. [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: |
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