المؤلفون: Singh, Sangeeta B.¹, Mahajan, Shikhaa², Singh, S. P.³, Khan, Farah Deeba⁴, Kumari, Asha⁵, Agrawal, Yuthika⁵

المصدر: Journal of Cardiovascular Disease Research (Journal of Cardiovascular Disease Research). 2023, Vol. 14 Issue 4, p2303-2308. 6p.

مصطلحات موضوعية: *HYPERTENSION in pregnancy, *LACTATE dehydrogenase, *ECLAMPSIA, *PREGNANCY complications, *PREECLAMPSIA, *HYPERTENSION

مستخلص: Background: Hypertensive disorders in pregnancy are one of the common medical complications of pregnancy and contributes significantly to maternal and perinatal morbidity and mortality. Lactate dehydrogenase (LDH) is an enzyme that catalyses the oxidation of L lactate to pyruvate. LDH levels are increased in the scenario of damaged tissues, increased cell leakiness, hemolysis and cellular damage caused by pregnancy induced hypertension and its severity. Objective: To estimate and compare the levels of serum lactate dehydrogenase in pregnancy induced hypertension (PIH) cases and controls. Materials and Methods: A hospital-based case control study was conducted in SHKM GMC Nuh Haryana between August 2021 to July 2022, on 90 cases of PIH (Mild preeclampsia, Severe preeclampsia and Eclampsia) and 90 controls aged 18-45 years females visiting the hospital, following inclusion and exclusion criteria and willing to participate. Samples were collected and analyzed for serum LDH by fully automated analyzer using spectrophotometry. Results: Mean values of serum LDH levels in Eclampsia were (1365.17±275.22 U/L), severe preeclampsia was (791.13±93.96 U/L) and mild preeclampsia were (422.37±72.13 U/L), while in controls it was (193.71±34.12 U/L). On comparing statistically, a significant difference was observed between the groups with p value <0.001. Conclusion: LDH levels can be used to assess the extent of cellular death and thereby the severity of disease so as to prevent progression of preeclampsia to eclampsia. [ABSTRACT FROM AUTHOR]

المؤلفون: Kurakula, Lavanya¹, Lakshmi, Audi Bhagya², Naweed, Asra¹

المصدر: Journal of Cardiovascular Disease Research (Journal of Cardiovascular Disease Research). 2023, Vol. 14 Issue 2, p404-407. 4p.

مصطلحات موضوعية: *CREATINE kinase, *LACTATE dehydrogenase, *ASTHMATICS, *WHEEZE, *RESPIRATORY obstructions, *ASTHMA

مصطلحات جغرافية: HYDERABAD (Pakistan), INDIA

مستخلص: Background and Objective: The research measures blood creatine kinase, lactate dehydrogenase, in asthmatics with exacerbation and stable asthma. The research will link these levels and predict asthma severity to assist clinicians to diagnose and treat asthma and avoid abrupt and severe airway blockage in patients with hyperirritability of airways and other allergic disorders, who are at high risk for deadly asthma. Method: Department of Biochemistry, Osmania general hospital, Hyderabad, Telangana, India, conducted the investigation on 80 asthmatics and 40 controls who participated in the research. 40 instances were stable asthma and 40 aggravating asthma. SPSS 17.0 was used to statistically examine the data. Result: LDH and CK are higher in exacerbating asthma patients compared to stable asthma cases and controls. Conclusion: In order to help doctors in the diagnosis and treatment of asthma and avoid abrupt and severe airway obstruction in hyperirritable airways, the current research examined blood creatine kinase, serum lactate dehydrogenase, in aggravating and stable asthma patients. [ABSTRACT FROM AUTHOR]

المؤلفون: Babu, Koppukonda Ravi¹, Shanthi, Bonagiri², Sridevi, D.³

المصدر: Journal of Cardiovascular Disease Research (Journal of Cardiovascular Disease Research). 2023, Vol. 14 Issue 2, p344-349. 6p.

مصطلحات موضوعية: *C-reactive protein, *LACTATE dehydrogenase, *COVID-19, *FERRITIN, *FIBRIN fragment D

مصطلحات جغرافية: HYDERABAD (India), INDIA

مستخلص: Background and Objectives: C-reactive protein (CRP) is an acute-phase protein that manifests in the blood within 6-10 hours in severe states of COVID-19 hyper inflammatory response, which results in a pathological dysfunction of innate host defense mechanisms. One of the biomarkers of inflammation in bacterial or viral infection is the iron storage form known as ferritin. In this retrospective study, our goal was to assess the diagnostic efficacy of CRP, ferritin, LDH, and D-dimer in identifying COVID-19 positive cases in India. Methods: This research was carried out retrospectively at the Malla Reddy Institute of Medical Sciences in Hyderabad, India. A tertiary care teaching institution's electronic medical records from July 2020 and May 2021 were examined for the test results for CRP, LDH, ferritin, and D dimer in 172 patients overall. Results: The results in ROC show that when all these parameters are combined, the area under the curve (AUC) of LDH and D-dimer is 0.62, that of LDH and C-reactive protein is 0.68, that of LDH and Ferritin is 0.96, and that of LDH, FERRITIN, D-DIMER, and C-reactive protein is 0.99. Before moving on to a final RT-PCR diagnosis, the severity of COVID-19 can be evaluated using a combination of common laboratory biomarkers (CRP, LDH, ferritin, and D- dimer) with a recognized sensitivity and specificity. Conclusion: Prior to moving forward with a formal diagnosis by RT-PCR, a combination of common laboratory biomarkers (CRP, LDH, ferritin, and D-dimer) can be used to predict the diagnosis of COVID-19 with a recognized sensitivity and specificity. [ABSTRACT FROM AUTHOR]

المؤلفون: H., Sujith¹, Udupa, Aniruddha², Y., Madhu Sudhan¹

المصدر: Journal of Cardiovascular Disease Research (Journal of Cardiovascular Disease Research). 2022, Vol. 13 Issue 7, p671-681. 11p.

مصطلحات موضوعية: *PLEURAL effusions, *LACTATE dehydrogenase, *CHOLESTEROL, *DIAPHRAGM walls, *MEDICAL sciences

مستخلص: Background: Prevalence of Pleural effusion is approximately 400/100000 population. Pleural effusion can manifest as a result of several diseases, both pulmonary and extrapulmonary. The pleura is susceptible to infections due to its direct apposition to other structures. For example, the parietal pleura over the diaphragm and chest wall is resistant to infection, whereas the parietal pleura lying over the mediastinum can be easily invaded by organisms. OBJECTIVES: 1. To determine pleural fluid Cholesterol and LDH from pleural fluid analysis. 2. To determine the cut-off levels for pleural fluid cholesterol and LDH in our set up, given the variability of results from one laboratory to another. MATERIAL & METHODS: Study Design: Prospective hospital based cross - sectional study. Study area: The present study was conducted in the department of TB & Respiratory medicine, Subbaiah Institute of Medical Sciences, Shimoga, Karnataka. Study Period: Mar. 2021 - Feb. 2022. Study population: Patients with Pleural Effusion evaluated in Department of Respiratory Medicine in Subbaiah Institute of Medical Sciences, Shimoga, Karnataka. Statistical analysis: Analysis of variance (ANOVA) has been used to find the significance of study parameters between three or more groups of patients, Student t test (two tailed, independent) has been used to find the significance of study parameters on continuous scale between two groups (Inter group analysis) on metric parameters. Results: Analysing pleural fluid LDH and Cholesterol in exudative pleural effusions using student t test mean LDH level 772.52 + 144.56 was moderately significant and mean cholesterol level 81.36 + 5.30 was strongly significant. Analysing pleural fluid LDH and Cholesterol in transudative pleural effusions using student t test mean LDH level 116.06 + 32.89 was moderately significant and mean cholesterol level 26.25 + 3.64 was strongly significant. CONCLUSION: From our study it can be concluded that, Pleural fluid cholesterol and LDH were found to be excellent pleural fluid parameters for differentiating exudative and transudative effusions from the study. They are both cost effective as well as technically feasible to analyse and hence could serve as better guides in the proper management of pleural effusion. [ABSTRACT FROM AUTHOR]

المؤلفون: sai, K. S. R. Krishna¹, Saketh, K.², koll, Harika³ harikakolli23@gmail.com

المصدر: Journal of Cardiovascular Disease Research (Journal of Cardiovascular Disease Research). 2023, Vol. 14 Issue 8, p239-243. 5p.

مصطلحات موضوعية: *BIOMARKERS, *LIVER enzymes, *LACTATE dehydrogenase, *CARCINOMA, *PUBLIC hospitals

مصطلحات جغرافية: ANDHRA Pradesh (India)

مستخلص: Introduction: Cancer is the second largest killer disease. Cancer Of the breast is common in women in developed countries and more than 40% of all breast cancer cases found in developing countries. The present study was done for the early detection of carcinoma. Methodology: The present study was conducted in the Department of Biochemistry, Rangaraya Medical College, Kakinada, and Andhra Pradesh, India. The present study was undertaken to determine biochemical changes in60 carcinoma breast cases. 60 cases were divided into 2groups-a. Premenopausal 30 cases, and b. Post-menopausal 30 cases, who were newly diagnosed and untreated. These values are compared with40 healthy controls. All of these subjects were taken from surgery department, Government General Hospital, Kakinada. R.B.S., Total Proteins, Albumin, A/G ratio, lactate dehydrogenase, gamma glutamyl transferase and liver enzymes (T. Bilirubin, SGOT, SGPT, ALP) were estimated among cases and controls. Results:. Mean values of LDH, GGT, PROTEINS, ALP were significantly increased in all the cases when compared with controls. Conclusion: These findings suggest that the estimation of less specific biomarkers like LDH, GGT and ALP can be used as routine tests in all suspected carcinoma breast patients because these can be easily assayed, approached for general population, less expensive and can detect metastasis also. [ABSTRACT FROM AUTHOR]

المؤلفون: Sujeev N.¹, Mohamed, Shamil¹, Thulasi P.², Jothipriya B.³ joe9491@gmail.com, Mohan, Amala P.⁴, Kumar, R. C. Krishna⁵

المصدر: Journal of Cardiovascular Disease Research (Journal of Cardiovascular Disease Research). 2022, Vol. 13` Issue 8, p523-528. 6p.

مصطلحات موضوعية: *URIC acid, *SQUAMOUS cell carcinoma, *LACTATE dehydrogenase

مستخلص: Background: To assess serum and salivary lactate dehydrogenase and uric acid levels in oral squamous cell carcinoma patients (OSCC). Material and Methods: Seventy- five patients diagnosed with OSCC of either gender was put in group I and equal number of healthy controls without OSCC in group II. From all, 5 ml of venous blood samples was collected. LDH in serum and saliva was performed using a semi-automatic analyser machine. The serum and salivary uric acid levels were determined using the uricase-Trinder end point method. Results: Group I comprised of 55 males and 20 females and group II had 40 males and 35 females. The mean salivary LDH level in group I was 640.3 U/L and in group II was 256.4 U/L. The difference was significant (P< 0.05). The mean serum LDH level in group I was 346.8 U/L and in group II was 250.0 U/L. The difference was significant (P< 0.05). The mean serum uric acid in group I was 6.92 mg/dl and in group II was 51.4 mg/dl. The difference was significant (P< 0.05). The mean salivary uric acid in group I was 5.96 and in group II was 5.20. The difference was significant (P< 0.05). Conclusion: All patients diagnosed with oral squamous cell carcinoma exhibited increased level of serum and salivary LDH and uric acid. [ABSTRACT FROM AUTHOR]

المؤلفون: Chakrabarti, Samrat¹, Acharya, Rama Krishna¹, Biswas, Sujoy¹, Sardar, Rakhi¹, Shil, B. N.²

المصدر: Journal of Cardiovascular Disease Research (Journal of Cardiovascular Disease Research). 2022, Vol. 13 Issue 7, p647-655. 9p.

مصطلحات موضوعية: *ECLAMPSIA, *BIOMARKERS, *LACTATE dehydrogenase, *ENDOENZYMES, *PREGNANT women, *PYRUVIC acid

مستخلص: Background: In the course of glycolysis, the intracellular enzyme lactate dehydrogenase (LDH) transforms pyruvic acid into lactic acid. The main source of energy in the placenta is glycolysis. In PE, hypoxia further boosts glycolysis and raises LDH activity. Studies have revealed that PE placentas had more LDH activity and gene expression than placentas from healthy pregnancies. OBJECTIVES: To compare serum LDH levels in the normal pregnant women and in women with preeclampsia and eclampsia in the antepartum period. To study the correlation of maternal and perinatal outcomes with serum LDH levels. Material & Methods: Study Design: Prospective hospital based cross - sectional study. Study area: study conducted in the Department of Obstetrics and Gynaecology in collaboration with the department of Biochemistry, Murshidabad Medical College, Berhampore, Murshidabad, West Bengal. Study Period: June 2021 - May 2022. Study population: Pregnant women in the third trimester were enrolled in the study. Sample size: study consisted a total of 100 cases and 100 controls. Sampling Technique: Simple Random sampling method. Study tools and Data collection procedure: The rest of the women were enrolled in the study and divided into the following groups Group 1: Healthy normal pregnant women (controls) Group 2: Patients of preeclampsia and eclampsia (c a s e s). They were subdivided into two groups based on severity of preeclampsia 2a: Patients with non-severe preeclampsia 2b: Patients with severe pre-eclampsia. Results: In the control group, all had levels of LDH < 600 IU/L, the mean value of LDH being 395.16 ± 92.54 IU/L. Majority of the patients (77.4%) in the non-severe preeclampsia group had LDH levels < 600 IU/L, 6 (9.2%) patients had LDH in the range of 600-800 IU/L, 8(12.9%) patients had LDH in the range of > 800 IU/L. The mean LDH Level in the nonsevere preeclampsia group is 513 ± 306.08. CONCLUSION: A helpful biochemical indicator of the severity and occurrence of preeclampsia problems is lactic dehydrogenase. These issues may be avoided by identifying high-risk patients with increased levels of lactic dehydrogenase, closely monitoring them, and providing timely, appropriate care, which will reduce maternal and foetal morbidity and mortality. [ABSTRACT FROM AUTHOR]

المؤلفون: Bais, Bhavana¹

المصدر: Journal of Cardiovascular Disease Research (Journal of Cardiovascular Disease Research). 2024, Vol. 15 Issue 2, p1045-1049. 5p.

مصطلحات موضوعية: *CANCER patients, *BREAST cancer, *LIVER enzymes, *LACTATE dehydrogenase, *ALKALINE phosphatase

مستخلص: Background: Breast cancer is a type of cancer that originates in the cells of the breast tissue. The present study was conducted to assess serum LDH and Gamma GT in breast cancer patients. Materials & Methods: 80 female subjects were divided into 2 groups of 40 each. Group I was cases with breast cancer who were further divided into premenopausal and postmenopausal. Group II was control subjects. Biochemical investigations like serum Lactate dehydrogenase, Gamma Glutamyl Transpeptidase, Alkaline phosphatase, liver enzymes (SGOT, SGPT, & ALP), and total proteins were calculated. Results: The age group 20-45 years (premenopausal) had 18 patients in group I and 20 subjects in group II and >45 years had 22 post-menopausal patients in group I and 20 subjects in group II. The difference was non-significant (P> 0.05). Total protein (g/dl) in premenopausal, post-menopausal, and in group II subjects was 7.4, 8.6, 6.2 respectively. The mean albumin (g/dl) was 2.8, 3.0, and 2.4 respectively. The mean globulin (g/dl) levels were 4.6, 5.6, and 3.8 respectively. The mean LDH (U/L) level was 512.4, 536.4 and 290.4 respectively. The mean GGT (IU/L) level was 50.2, 84.6 and 24.6 respectively. The mean ALP (IU/L) was 122.5, 180.4, and 43.1 respectively. The mean SGOT (U/L) level was 62.1, 75.3 and 34.6. The mean SGPT (U/L) level was 68.5, 69.2, and 36.2 respectively. Conclusion: The level of LDH, GGT, and ALP was significantly higher in breast cancer patients and hence, can be used as routine screening tests in all suspected carcinoma breast patients. [ABSTRACT FROM AUTHOR]

المؤلفون: Badbade, Padmaj¹ drpadmaj17@gmail.com, Bhandare, Samuel², Kudache, Abhaykumar²

المصدر: Journal of Cardiovascular Disease Research (Journal of Cardiovascular Disease Research). 2021, Vol. 12 Issue 6, p71-73. 3p.

مصطلحات موضوعية: *GASTRITIS, *SEPTIC shock, *LACTATE dehydrogenase, *ALKALINE phosphatase, *ALANINE aminotransferase

مستخلص: Phlegmonous gastritis (PG) is a rare, suppurative bacterial infection of the gastric wall. We report a case of a 45-year-old male who presented with severe epigastric and right upper quadrant abdominal pain. The serum alanine aminotransferase, total bilirubin, alkaline phosphatase, lactate dehydrogenase, amylase, and lipase were within normal ranges. On hospital day 10, the antibiotic regimen was changed to orally administered amoxicillin/clavulanic acid and clindamycin. The patient was discharged on hospital day 11. This case emphasized the importance of early diagnosis of this potentially fatal infection that can follow endoscopic procedures and illustrates ACS and septic shock as serious complications. [ABSTRACT FROM AUTHOR]

المؤلفون: Kanhar, Manabhanjan Bhimasingh¹, Meher, Roshan Kumar², Sahoo, Kumuda Bandhu³, Mohapatra, Abinasha⁴

المصدر: Journal of Cardiovascular Disease Research (Journal of Cardiovascular Disease Research). 2023, Vol. 14 Issue 10, p44-52. 9p.

مصطلحات موضوعية: *BOWEL obstructions, *LACTATES, *STRANGLING, *LACTATE dehydrogenase, *INTESTINAL ischemia, *LONGITUDINAL method, *MESENTERIC ischemia

مستخلص: Background - Laboratory tests are essential in patients with bowel obstruction because they may aid in the diagnosis and more importantly any underlying metabolic defects should be corrected prior to operative therapy. While no rest in sensitive and specific enough to diagnose mesenteric ischemia reliably, a spectrum of laboratory tests may be helpful in determining the condition of the patient and should guide resuscitation. Arterial blood pH, serum lactate concentrations and amylase and lactic dehydrogenase activity may be useful tests in the evaluation of bowel obstruction, especially when trying to exclude the presence of strangulation or underlying bowel necrosis. An increase in serum lactate concentrations should raise the suspicion of intestinal ischemia. Intestinal fatty acid binding protein (I-FABP) is a highly sensitive marker for extensive mesenteric infarction. Material and Methods - The aim of this prospective observational study is to evaluate the role of Serum Lactate as a marker of strangulation in bowel obstruction. 50 cases admitted in Emergency General Surgery ward, S.C.B. Medical College, Cuttack with clinical suspicion of intestinal obstruction for a period of 6 months (February 2021 to Feb 2022). Assessment and comparison of serum lactate levels in various outcomes of intestinal obstruction is done. Results - The mean serum lactate value in the patients were 5.16 mmol/L ranging between 1.70 to 8.60 mmol/L. The cut off values of strangulation, simple obstruction and those subacute cases managed conservatively were 4.3,3.4 and less than 2.3 respectively. Based on these values, 72% were classified as strangulation, 22% with strong suspicion and 6% of them were normal. Kruskal-Wallis test for comparison of serum lactate values across the three groups with normal, strong suspicion and strangulation shows that the results are significant with a chi-square value of 30.23 with p=0.00123(highly significant). Conclusion - A positive correlation between elevated serum lactate and strangulation bowel obstruction had been established, i.e. serum lactate levels were significantly raised in strangulated bowel obstruction as compared with simple bowel obstruction. [ABSTRACT FROM AUTHOR]