التفاصيل البيبلوغرافية
| العنوان: |
A high throughput toolbox for comprehensive flavor compound mapping in mint. |
| المؤلفون: |
Peters, Verena Christina Tabea1 (AUTHOR), Dunkel, Andreas2 (AUTHOR), Frank, Oliver1 (AUTHOR), McCormack, Brian3 (AUTHOR), Dowd, Eric3 (AUTHOR), Didzbalis, John4 (AUTHOR), Dawid, Corinna1 (AUTHOR) corinna.dawid@tum.de, Hofmann, Thomas1 (AUTHOR) |
| المصدر: |
Food Chemistry. Dec2021, Vol. 365, pN.PAG-N.PAG. 1p. |
| مصطلحات موضوعية: |
*LIQUID chromatography-mass spectrometry, *FLAVOR, *ESSENTIAL oils, *ATMOSPHERIC pressure, *OIL wells, *TERPENES |
| مستخلص: |
• high-throughput quantification of aroma compounds in mint by SIDA–UPHLC–MS/MS. • bead-beater homogenization approach for single mint leaves. • APCI, 3-NPH and GTMA derivatization used to ensure UHPLC–MS/MS analysis. • Enables characterizing of aroma compounds in hundreds of mint samples. Essential oils of the genus Mentha are extensively used as flavor ingredients in the industry. To overcome the time consuming and laborious traditional flavor analysis, a new quick, high–throughput toolbox based on a bead–beater homogenization followed by a UHPLC–MS/MS analysis has been developed and validated. While terpenes could be directly detected using atmospheric pressure chemical ionization (APCI), carbonyl compounds and alcohols required derivatization by 3–nitrophenylhydrazine (3-NPH) and glycidyltrimethylammonium chloride (GTMA) to ensure sufficient sensitivity for analysis of a single leaf. Using this approach, in total, 59 flavor-active metabolites representing the characteristic flavor of mint were quantified in leaves as well as in distilled oils using fast and robust UHPLC–MS/MS methods. The application of this toolbox enables a mapping of key pathways of mint flavor biosynthesis and can therefore support extensive breeding studies and the monitoring of chemosensate changes, depending on factors such as growth stages and environmental conditions. [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: |
Academic Search Index |
