التفاصيل البيبلوغرافية
| العنوان: |
Comparative diagnostic efficacy of recombinant LLO and PI-PLC-based ELISAs for detection of listeriosis in animals. |
| المؤلفون: |
Suryawanshi, Rahul D.1, Malik, Satya Veer Singh1, Jayarao, Bhushan2, Chaudhari, Sandeep P.3, Savage, Emily2, Vergis, Jess1, Kurkure, Nitin V.3, Barbuddhe, Sukhadeo B.4, Rawool, Deepak B.1 deepak.rawool@yahoo.com |
| المصدر: |
Journal of Microbiological Methods. Jun2017, Vol. 137, p40-45. 6p. |
| مصطلحات موضوعية: |
*LISTERIOSIS, *LISTERIOLYSIN O, *PHOSPHATIDYL inositol, *ENZYME-linked immunosorbent assay, *LISTERIOSIS in animals, *SEROPREVALENCE, *DIAGNOSIS |
| مستخلص: |
The present study for the first time evaluates the serodiagnostic efficacy of two recombinant antigens namely, listeriolysin O (rLLO) and phosphatidyl-inositol phospholipase C (rPI-PLC). Indirect ELISA with the above recombinant antigens was used on samples collected from bovines ( n = 106), goats ( n = 138) and pigs ( n = 92) having either a history of abortion, emaciation and/or apparently healthy animals. Isolation of Listeria was attempted from the blood samples using USDA-FSIS method. On screening of test sera by rLLO-based ELISA, antibodies against anti-listeriolysin O (ALLO) were observed in goats (22.46%), bovines (15.10%) and pigs (16.31%). As advocated, after adsorption of positive serum samples with streptolysin O (SLO), the seropositivity for ALLO was marginally reduced ( p > 0.05) in goats (21.73%) and bovines (10.38%), whereas, in pigs the reduction (5.43%) was significant ( p < 0.05). On the contrary, rPI-PLC-based ELISA revealed higher non-specific seropositivity for antilisterial antibodies in goats (45.65%), bovines (31.13%) and pigs (8.69%). Further, on comparing the seropositivity with isolation rate, of the 16 animals that were culturally-positive for L. monocytogenes , 15 showed ALLO positivity in unadsorbed as well as SLO-adsorbed sera by rLLO-based ELISA, however, rPI-PLC-based ELISA could detect seropositivity in only 5 animals. Moreover, rPI-PLC-based ELISA also showed seropositivity in those animals (7/30) that were culturally positive for other Listeria spp. In conclusion, rLLO can serve as a better antigen than rPI-PLC in ELISA for the serodiagnosis of listeriosis in animals; however, prior adsorption of test sera with SLO is required to avoid false positive results. [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: |
Academic Search Index |
