التفاصيل البيبلوغرافية
| العنوان: |
Acquired stress resilience through bacteria‐to‐nematode interdomain horizontal gene transfer. |
| المؤلفون: |
Pandey, Taruna1 (AUTHOR), Kalluraya, Chinmay A2 (AUTHOR), Wang, Bingying1 (AUTHOR), Xu, Ting3 (AUTHOR), Huang, Xinya3 (AUTHOR), Guang, Shouhong3 (AUTHOR), Daugherty, Matthew D2 (AUTHOR) mddaugherty@ucsd.edu, Ma, Dengke K1,4 (AUTHOR) dengke.ma@ucsf.edu |
| المصدر: |
EMBO Journal. 12/11/2023, Vol. 42 Issue 24, p1-12. 12p. |
| مصطلحات موضوعية: |
*HORIZONTAL gene transfer, *CAENORHABDITIS elegans, *BACTERIAL enzymes, *ANIMAL exoskeletons, *NATURAL selection, *BACTERIAL genes |
| مستخلص: |
Natural selection drives the acquisition of organismal resilience traits to protect against adverse environments. Horizontal gene transfer (HGT) is an important evolutionary mechanism for the acquisition of novel traits, including metazoan acquisitions in immunity, metabolic, and reproduction function via interdomain HGT (iHGT) from bacteria. Here, we report that the nematode gene rml‐3 has been acquired by iHGT from bacteria and that it enables exoskeleton resilience and protection against environmental toxins in Caenorhabditis elegans. Phylogenetic analysis reveals that diverse nematode RML‐3 proteins form a single monophyletic clade most similar to bacterial enzymes that biosynthesize L‐rhamnose, a cell‐wall polysaccharide component. C. elegans rml‐3 is highly expressed during larval development and upregulated in developing seam cells upon heat stress and during the stress‐resistant dauer stage. rml‐3 deficiency impairs cuticle integrity, barrier functions, and nematode stress resilience, phenotypes that can be rescued by exogenous L‐rhamnose. We propose that interdomain HGT of an ancient bacterial rml‐3 homolog has enabled L‐rhamnose biosynthesis in nematodes, facilitating cuticle integrity and organismal resilience to environmental stressors during evolution. These findings highlight a remarkable contribution of iHGT on metazoan evolution conferred by the domestication of a bacterial gene. Synopsis: Interdomain horizontal gene transfer (iHGT) between species can sometimes occur in evolution. Here, phylogenetic and experimental analyses reveal a C. elegans gene originating from bacteria that is crucial for exoskeleton resilience against environmental toxins. Evolutionary phylogenetic evidence supports iHGT from bacteria to nematodes for the C. elegans gene rml‐3.The RML‐3 protein participates in L‐rhamnose biosynthesis and is regulated during C. elegans development and upon environmental stress in the cuticle.RML‐3 promotes exoskeletal cuticle integrity, hypodermal barrier functions, and organismal stress resilience against environmental toxins. [ABSTRACT FROM AUTHOR] |
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