Data from: Forward genetic screening for regulators involved in cholesterol synthesis using validation-based insertional mutagenesis
| العنوان: | Data from: Forward genetic screening for regulators involved in cholesterol synthesis using validation-based insertional mutagenesis |
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| المؤلفون: | Jiang, Wei, Tang, Jing-Jie, Miao, Hong-Hua, Qu, Yu-Xiu, Qin, Jie, Xu, Jie, Yang, Jinbo, Li, Bo-Liang, Song, Bao-Liang |
| سنة النشر: | 2014 |
| المجموعة: | Zenodo |
| مصطلحات موضوعية: | somatic cell genetics, SREBP, forward genetic screen, HMG-CoA reductase, VBIM, Cholesterol, SCAP |
| الوصف: | Somatic cell genetics is a powerful approach for unraveling the regulatory mechanism of cholesterol metabolism. However, it is difficult to identify the mutant gene(s) due to cells are usually mutagenized chemically or physically. To identify important genes controlling cholesterol biosynthesis, an unbiased forward genetics approach named validation-based insertional mutagenesis (VBIM) system was used to isolate and characterize the 25-hydroxycholesterol (25-HC)-resistant and SR-12813-resisitant mutants. Here we report that five mutant cell lines were isolated. Among which, four sterol-resistant mutants either contain a truncated NH2-terminal domain of sterol regulatory element-binding protein (SREBP)-2 terminating at amino acids (aa) 400, or harbor an overexpressed SREBP cleavage-activating protein (SCAP). Besides, one SR-12813 resistant mutant was identified to contain a truncated COOH-terminal catalytic domain of 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMG-CoA reductase). This study demonstrates that the VBIM system can be a powerful tool to screen novel regulatory genes in cholesterol biosynthesis. ; SL-4 genome sequenceGene locus of SCAP in SL-4 mutant. This sequence is related to Fig.3SL-4 genome seq.docx |
| نوع الوثيقة: | dataset |
| اللغة: | unknown |
| العلاقة: | https://zenodo.org/communities/dryadTest; https://zenodo.org/record/4992737Test; https://doi.org/10.5061/dryad.p100gTest; oai:zenodo.org:4992737 |
| DOI: | 10.5061/dryad.p100g |
| الإتاحة: | https://doi.org/10.5061/dryad.p100gTest https://doi.org/10.1371/journal.pone.0112632Test https://zenodo.org/record/4992737Test |
| حقوق: | info:eu-repo/semantics/openAccess ; https://creativecommons.org/publicdomain/zero/1.0/legalcodeTest |
| رقم الانضمام: | edsbas.B4EB0FD1 |
| قاعدة البيانات: | BASE |
| DOI: | 10.5061/dryad.p100g |
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