Low dose rate radiation induced secretion of TGF-β3 together with an activator in small extracellular vesicles modifies low dose hyper-radiosensitivity through ALK1 binding
| العنوان: | Low dose rate radiation induced secretion of TGF-β3 together with an activator in small extracellular vesicles modifies low dose hyper-radiosensitivity through ALK1 binding |
|---|---|
| المؤلفون: | Hanson, Ingunn |
| المساهمون: | Edin, Nina F. J. |
| سنة النشر: | 2022 |
| المجموعة: | Zenodo |
| مصطلحات موضوعية: | Adaptive response, ALK1, ALK5, Bystander effects, Hyper-radiosensitive response, Low-dose radiation, Low dose rate, TGF-β3 |
| الوصف: | This is a collection of results from all clonogenic assays on T-47D cells used in the manuscript "Low dose rate radiation induced secretion of TGF-β3 together with an activator in small extracellular vesicles modifies low dose hyper-radiosensitivity through ALK1 binding". T-47D cells were subjected to various pretreatments: low dose rate priming (0.1-0.3 Gy/h for 1 hour), small extracellular vesicles from irradiated or control cells, irradiated or control cell conditioned medium, MMP/ADAM inhibitor TAPI-2, recombinant TGF-B3, inhibitors of ALK1, ALK2, ALK5 or TGF-BRII, iNOS inhibitor 1400W, recombinant FKBP4, recombinant MMP14 and combinations of these. All pretreatments except low dose rate priming was administered for 24 hours. After pretreatments, cells were seeded to colonies and irradiated with 220 kV x-rays at a dose rate of 22.5 Gy/h or gamma rays from a Co-60 source at a dose rate of 20-25 Gy/h. Colonies were cultured for 2-3 weeks before fixation and manual counting. ; This work was supported by EU FP7 grant No. 222741 (METOXIA), the Research Council of Norway, the Norwegian Cancer Society and Eckbos Legater. |
| نوع الوثيقة: | dataset |
| اللغة: | English |
| العلاقة: | info:eu-repo/grantAgreement/EC/FP7/222741/; https://zenodo.org/record/6685318Test; https://doi.org/10.5281/zenodo.6685318Test; oai:zenodo.org:6685318 |
| DOI: | 10.5281/zenodo.6685318 |
| الإتاحة: | https://doi.org/10.5281/zenodo.6685318Test https://doi.org/10.5281/zenodo.6685317Test https://zenodo.org/record/6685318Test |
| حقوق: | info:eu-repo/semantics/openAccess ; https://creativecommons.org/licenses/by/4.0/legalcodeTest |
| رقم الانضمام: | edsbas.D7156AEB |
| قاعدة البيانات: | BASE |
| DOI: | 10.5281/zenodo.6685318 |
|---|