المؤلفون: Patil,Shital, Bhadake, Manojkumar, Narwade,Ganesh, Acharya,Abhijit

المصدر: Journal of One Health Research 1(1) 7-15

مصطلحات موضوعية: COVID-19, pneumonia, lactate dehydrogenase

الوصف: Objective: The role of various inflammatory markers has been documented during the evaluation of COVID-19 pneumonia. In the present study, the role of lactate dehydrogenase (LDH) in COVID-19 pneumonia in predicting severity, oxygenation status and confirming response to interventions and final radiological outcome was examined. Methods: This observational study included 1000 PCR-confirmed COVID-19 cases. All cases were assessed with lung involvement documented and categorized on thorax computer tomography, oxygen saturation, inflammatory marker as LDH at the entry point and follow-up. Age, gender, comorbidity and use of bi-level positive airway pressure/noninvasive ventilation (BPAP/NIV) and outcome as with or without lung fibrosis as per tomography severity were observed. Results: In the study of 1000 COVID-19 pneumonia cases. Tomography severity score at the entry point has significantly associated with LDH level (p<0.001). LDH level has a significant association with the duration of illness and oxygen saturation at the entry point (p<0.001). BPAP/NIV requirement during the course of hospitalization has a significant association with LDH level (p<0.001). Follow-up LDH titer during hospitalization as compared to entry point abnormal LDH has a significant association in post-covid lung fibrosis (p<0.001). Follow-up LDH titer during hospitalization as compared to entry point normal LDH has a significant association in post-covid lung fibrosis (p<0.001). Conclusion: LDH has documented a important role in COVID-19 pneumonia in predicting the severity of illness and progression of pneumonia. Sequential LDH titers will help assess response to treatment during hospitalization and analyse post-covid lung fibrosis.

العلاقة: https://zenodo.org/record/7521677; https://doi.org/10.5281Test/zenodo.7521677; oai:zenodo.org:7521677

الإتاحة: https://doi.org/10.5281Test/zenodo.7521677
https://doi.org/10.5281Test/zenodo.7521676
https://zenodo.org/record/7521677

المؤلفون: Hugo Eduardo Guerrero-Topete, Ma. Natalia Gómez-González, Carlos Jiménez-Correa, Martha Alicia Hernández-González, Juan Pablo Fernández-Hernández, Pedro Luis González-Carrillo

المصدر: International Journal of Medical Science and Clinical Research Studies 02(05) 322-330

مصطلحات موضوعية: SARS-CoV-2, lactate dehydrogenase, Severity, Pneumonia, Mexico

الوصف: Serum lactate dehydrogenase (LHD) concentration has been described as a biomarker to predict severe lung injury and severe hypoxemia in patients with ARDS. The aim of the study was to correlate lactate dehydrogenase concentrations with the tomographic severity index in SARS-CoV-2 pneumonia cases, considering lung CT as the gold standard for severity diagnosis in this population based on the presence of ground glass pattern and the score given in the segmental assessment. A total of 110 patients were included in the study, in the period from April to September 2020. From the results, a moderate correlation was obtained between serum LHD levels and severity score by pulmonary tomography, with an r=0.474 and p <0.0001; with a severity-associated serum LHD cut-off point of 396 mg /dl (p < 0.001, sensitivity 86.02%, specificity 75%, AUC 0.806). Serum DHL levels may be useful for predicting severity in patients with COVID pneumonia 19t.

العلاقة: https://zenodo.org/record/6523887; https://doi.org/10.5281Test/zenodo.6523887; oai:zenodo.org:6523887

الإتاحة: https://doi.org/10.5281Test/zenodo.6523887
https://doi.org/10.5281Test/zenodo.6523886
https://zenodo.org/record/6523887

المؤلفون: Cheraghi pour, Mohammad, Khaleghi, Fatemeh, Namiranian, Nasim, Ansari, Kazem, Mansouri, Mehrdad, Injinari, Nastaran, Aghaeimeybodi, Fatemeh

المصدر: Journal of Advances in Medical and Biomedical Research 30(140) 215-222

مصطلحات موضوعية: COVID-19, Erythrocyte sedimentation rate, High-resolution computed tomography, Lactate dehydrogenase

الوصف: Background and Objective: Measurement of inflammatory markers and lactate dehydrogenase (LDH) may contribute to the evaluation of lung involvement severity. This study aimed to evaluate relationship between severity of primary lung involvement with highest level of erythrocyte sedimentation rate (ESR) and LDH in patients with COVID-19. Materials and Methods: This descriptive-analytical study was conducted on 123 patients with COVID-19 in Shahid Sadoughi Hospital. Data including age, gender, ESR (mm/h), LDH (U/L), and high-resolution Computed Tomography scan (HRCT) findings and hospitalization ward were extracted from medical records. The regression model was used to determine the relation between HRCT findings with LDH and ESR. Results: Mean LDH, ESR, and HRCT findings were 508.41±224.65, 52.23±29.56, and 37.17± 22.18 respectively. A significant relation was seen between HRCT findings with highest level of LDH and ESR (P=0.001). A significant relation was observed between the highest levels of ESR and HRCT findings, regarding age, gender, and hospitalization wards (P<0.01). There was a significant relation between the highest level of LDH and HRCT findings regarding age group and hospitalization wards (P<0.01). Conclusion: A significant relation was seen between HRCT findings and highest levels of ESR and LDH in patients with COVID-19. Therefore, it seems that assessment of laboratory findings such as LDH and ESR can be helpful as cost-effective markers instead of chest CT scan for predicting the severity of lung injury when the CT scan report is controversial. The relation between HRCT findings with LDH and ESR were affected by age and hospitalization ward. However, more studies should be conducted in this regard. Keywords: COVID-19, Erythrocyte sedimentation rate, High-resolution computed tomography, Lactate dehydrogenase

العلاقة: https://zenodo.org/record/6552310; https://doi.org/10.30699/jambs.30.140.215Test; oai:zenodo.org:6552310

الإتاحة: https://doi.org/10.30699/jambs.30.140.215Test
https://zenodo.org/record/6552310

المؤلفون: García, Jorge, Chapa, Christian

مصطلحات موضوعية: oxidoreductase, biochemistry, Enzymes, enzymatic activity, EC 1.1.1.27, Lactate dehydrogenase, Heart attack, bioquímica, actividad enzimática, enzimas, oxidorreductasa, Lactato deshidrogenasa, Ataque al corazón

الوصف: (EN) Description of the enzymatic activity of Lactate dehydrogenase according to the EC number (Enzyme Commission number) EC 1.1.1.27 and relationship of the enzyme with heart attack. (SP) Descripción de la actividad enzimática de la Lactato deshidrogenasa según el número CE (número de la Comisión de Enzimas) EC 1.1.1.27 y relación de la enzima con el ataque al corazón. ; (EN) "The video is an exercise by Biomedical Engineering students from the Autonomous University of Ciudad Juárez, in which information is compiled of databases such as BRENDA, 5MinuteConsult, ExPASy, IntEnz, etc. about enzymes and their relationship with diseases. The content of this video is not intended to give medical advice, nor to take the place of the medical advice or treatment of your physician, it is not a substitute for professional medical care, nor for a diagnosis. This information is not intended to diagnose, treat, cure or prevent any disease. The information and opinions, articles, products or recordings are for informational purposes only and are intended for the general public and students. " Feedback and comments are welcome in the email: christian.chapa@uacj.mx | (SP) "El video es un ejercicio de estudiantes de Ingeniería Biomédica de la Universidad Autónoma de Ciudad Juárez, en la que se compila información de bases de datos como BRENDA, 5MinuteConsult, ExPASy, IntEnz, etc. acerca de enzimas y su relación con enfermedades. El contenido de este video no pretende dar consejos médicos, ni tomar el lugar del consejo médico o tratamiento de su médico personal, no es un sustituto a un cuidado médico profesional directo o sustituto, ni a una diagnosis. Esta información no pretende diagnosticar, tratar, curar o prevenir ninguna enfermedad. La información y opiniones, artículos, productos o grabaciones tienen únicamente un propósito informativo y van dirigidas al público en general y estudiantes." Su retroalimentación y comentarios son bien recibidos en el correo: christian.chapa@uacj.mx

العلاقة: https://zenodo.org/record/4904030; https://doi.org/10.5281Test/zenodo.4904030; oai:zenodo.org:4904030

الإتاحة: https://doi.org/10.5281Test/zenodo.4904030
https://doi.org/10.5281Test/zenodo.4904029
https://zenodo.org/record/4904030

المؤلفون: Arushi Mohan, Padmini SN, Brunda MS, Abhinaya Shekhar, Paul Matthew, G.R. Lingeshwaran

المصدر: World Journal of Advanced Research and Reviews 11(1) 081-090

مصطلحات موضوعية: D-dimer, C-Reactive Protein, Ferritin, Lactate Dehydrogenase, Intensive Care Units, COVID-19

الوصف: Background: COVID-19 is a novel disease triggered by the SARS-CoV-2 virus, a beta coronavirus similar to MERS-CoV and SARS CoV. Inflammatory markers have a vital role in the pathogenesis of nCOVID 19; understanding the importance of these inflammatory markers in determining disease status is essential given the impact of the disease on healthcare. Thus, being able to triage cases with minimal tests is momentous to capture, which we have investigated as per our study guidelines of the role of inflammatory markers such as D-dimer, CRP (C - reactive protein), Ferritin, LDH (Lactate Dehydrogenase) in patients with COVID 19. In addition, limited data is available comparing the utility of these inflammatory markers to predict the following parameters as the need for ICU, oxygen support requirement, and duration of in-hospital stay, which can help guide the management protocol. Aim: This study aims to determine markers associated with poor prognosis in patients with Coronavirus disease 2019 (COVID-19). Objectives: 1) To assess the inflammatory markers that are routinely investigated in COVID- 19 patients. 2) To determine the most probable factor to estimate severity in COVID- 19 and thus predict prognosis. Methods: This is a retrospective cross-sectional observational study of patients who tested SARS COV 2 positive by RT PCR. The laboratory inflammatory markers, namely Lactate Dehydrogenase, C reactive protein, D-dimer, Ferritin, were assessed in the selected patients, and their clinical data and demographic details were taken into account. The parameters considered for contributing to the severity included the number of days of stay in the hospital, oxygen requirement, and ICU needs. Analyses relied upon analysis of variance for cross-sectional study design and a P< 0.05 statistical significance criterion. Results: There was a statistically significant difference found between oxygen requirement and D dimer (p<0.001), LDH (p= 0.002), and CRP (p= 0.024). There was a statistically significant difference found ...

العلاقة: https://zenodo.org/communities/wjarr; https://zenodo.org/record/5169042; https://doi.org/10.5281Test/zenodo.5169042; oai:zenodo.org:5169042

الإتاحة: https://doi.org/10.5281Test/zenodo.5169042
https://doi.org/10.30574/wjarr.2021.11.1.0300Test
https://doi.org/10.5281Test/zenodo.5169041
https://zenodo.org/record/5169042

المؤلفون: Pore, Minal M, Padwal, Meghana K, Raichurkar, Annapurna V

المصدر: Indian Journal of Medical Biochemistry 25(2) 83-87

مصطلحات موضوعية: Biomarkers, Coronavirus disease-2019, C-reactive protein, Ferritin, Lactate dehydrogenase, Procalcitonin

الوصف: Aims and objectives: The coronavirus disease-2019 (COVID-19) pandemic has led to an urgent need for reliable biomarkers to identify disease severity. Infection with COVID-19 is rapidly spreading posing a serious threat to community health. This study aims to identify the most effective biomarker among C-reactive protein, procalcitonin, lactate dehydrogenase, and ferritin to predict disease severity. The routine biochemical markers (LFT, RFT, electrolytes, CKNAC, CKMB, blood glucose level) were also evaluated for correlation with disease severity. Materials and methods: The present study is retrospective type. The study protocol was approved by IEC. The levels of biochemical markers of admitted COVID-19 positive patients were analyzed after determining the normal distribution and their significance was determined by calculating the p value. The accuracy of the biomarkers [C-reactive protein (CRP), PCT, lactate dehydrogenase (LDH), and ferritin] was checked using ROC analysis. Results: Five hundred and ninety-one COVID-19 positive patients admitted to the hospital (from May 2020 to December 2020) were considered for the study. Out of these 231 (39.1%) were admitted to the intensive care unit (ICU) and 360 (61%) were in-ward patients. The mean age of the study population was 50.39 ± 16.7, with ICU patients significantly older than non-ICU patients (p < 0.001) and 50–75 years being the most common age group. There was a statistically significant difference in the values of CRP, LDH, PCT, ferritin, AST, albumin, urea, CKNAC, sodium, potassium, and blood glucose levels (p value < 0.05). Conclusion: The severity of COVID-19 disease can be identified at an early stage by following the different routine biochemical marker levels and subsequently improve prognosis. Inflammatory markers (CRP, PCT, LDH, and ferritin) serve as useful guidance for determining disease severity in COVID-19 patients.

العلاقة: https://zenodo.org/communities/ijmb; https://zenodo.org/record/5816727; https://doi.org/10.5005/jp-journals-10054-0183Test; oai:zenodo.org:5816727

الإتاحة: https://doi.org/10.5005/jp-journals-10054-0183Test
https://zenodo.org/record/5816727

المؤلفون: Khushbun Nahar Layla, Shahanara Yeasmin, Sharif Ahmed Khan, Masba Uddin Chowdhury, Afrina Binte Azad, Farzana Yeasmin Mukta

المصدر: World Journal of Advanced Research and Reviews 11(2) 031-039

مصطلحات موضوعية: C-reactive protein, lactate dehydrogenase, Random blood glucose, Serum glutamic-pyruvic-transaminase, STA

الوصف: Background and objectives: Coronavirus disease 2019 (COVID-19) is affecting millions of people world-wide. It is caused by the severe acute respiratory syndrome corona virus 2(SARS-CoV-2). The laboratory findings are very important to assess the progress of the disease. The present study is aimed to discuss the biochemical parameters among mild, moderate and severe COVID-19 patients. Materials and methods: A cross sectional study were conducted in the Department of Physiology, Dhaka Medical College, Dhaka from January 2020 to December 2020. After obtaining ethical clearance, a total of 100 real time-polymerase chain reaction (RT-PCR) COVID-19 positive patients were selected from Dhaka Medical College Hospital. With all aseptic precautions, 10 ml of venous blood was collected from ante-cubital vein. D-dimer, prothrombin time, C-reactive protein (CRP), lactate dehydrogenase (LDH), serum ferritin, random blood glucose (RBG), serum creatinine, serum glutamic-pyruvic-transaminase (SGPT) and serum albumin measured in the Department of Laboratory Medicine, Dhaka Medical College Hospital, Dhaka. CRP was measured by Immunoturbidimetric method, serum ferritin was estimated by Chemiluminescent microparticle immunoassay. STA-neoplastine CI plus used with STA-R analyzer was used for determination of prothrombin time. D-dimer was estimated by Immunofluorescence Assay method. Serum LDH is measured by Dimention clinical chemistry system, serum albumin is measured by bromocresol purple dye binding method, serum creatinine is measured by Jaffe alkaline picrate method and serum SGPT is measured by colourmetric (IFCC 1980) method and RBG is measured by enzymatic colorimetric method (GOD-PAP). Data were recorded in a pre-designed structured data collection form. For statistical analysis, ANOVA followed by Bonferroni test, Chi square test, Spearman’s rho correlation coefficient test was performed as applicable using SPSS for windows version 25.0. Results: By analyzing biochemical parameters of mild, moderate and severe RT-PCR ...

العلاقة: https://zenodo.org/communities/wjarr; https://zenodo.org/record/5202893; https://doi.org/10.5281Test/zenodo.5202893; oai:zenodo.org:5202893

الإتاحة: https://doi.org/10.5281Test/zenodo.5202893
https://doi.org/10.30574/wjarr.2021.11.2.0348Test
https://doi.org/10.5281Test/zenodo.5202892
https://zenodo.org/record/5202893

المؤلفون: Dimitar Kovachev

المساهمون: Kovachev Dimitar

مصطلحات موضوعية: Covid-19, lactate dehydrogenase, neutrophils, urea

الوصف: Laboratory indicators for COVID-19 before the day of deterioration.

العلاقة: https://zenodo.org/record/3776155; https://doi.org/10.5281Test/zenodo.3776155; oai:zenodo.org:3776155

الإتاحة: https://doi.org/10.5281Test/zenodo.3776155
https://doi.org/10.5281Test/zenodo.3766429
https://doi.org/10.5281Test/zenodo.3783540
https://doi.org/10.5281Test/zenodo.3783544
https://doi.org/10.5281Test/zenodo.3776154
https://zenodo.org/record/3776155

المؤلفون: Nuzzo, Genoveffa, Landi, Simone, Esercizio, Nunzia, Manzo, Emiliano, Fontana, Angelo, d'Ippolito, Giuliana

المصدر: MDPI fermentation 5 (

مصطلحات موضوعية: optical purity, lactate dehydrogenase, stereochemistry, fermentation, thermophilic bacteria

الوصف: The industrial production of lactic acid (LA) is mainly based on bacterial fermentation. This process can result in enantiopure or racemic mixture according to the producing organism. Between the enantiomers, L-lactic acid shows superior market value. Recently, we reported a novel anaplerotic pathway called capnophilic lactic fermentation (CLF) that produces a high concentration of LA by fermentation of sugar in the anaerobic thermophilic bacterium Thermotoga neapolitana. The aim of this work was the identification of the enantiomeric characterization of the LA produced by T. neapolitana and identification of the lactate dehydrogenase in T. neapolitana (TnLDH) and related bacteria of the order Thermotogales. Chemical derivatization and GC/MS analysis were applied to define the stereochemistry of LA from T. neapolitana. A bioinformatics study on TnLDH was carried out for the characterization of the enzyme. Chemical analysis showed a 95.2% enantiomeric excess of L-LA produced by T. neapolitana. A phylogenetic approach clearly clustered the TnLDH together with the L-LDH from lactic acid bacteria. We report for the first time that T. neapolitana is able to produce almost enantiopure L-lactic acid. The result was confirmed by bioinformatics analysis on TnLDH, which is a member of the L-LDH sub-family.

العلاقة: info:eu-repo/grantAgreement/EC/H2020/760431/; https://zenodo.org/communities/bioreco2ver-h2020; https://zenodo.org/record/3245889; https://doi.org/10.3390/fermentation5020034Test; oai:zenodo.org:3245889

الإتاحة: https://doi.org/10.3390/fermentation5020034Test
https://zenodo.org/record/3245889

المؤلفون: Zahra A Ahmed, Abd Elkareem A Rabo, Asaad Ma. Babker

المصدر: GSC Biological and Pharmaceutical Sciences 9(3) 064-068

مصطلحات موضوعية: Lactate dehydrogenase, Gamma glutamyl transpeptidase, Breast cancer, Screaming test

الوصف: Breast cancer is cancer that forms in the cells of the breasts. After skin cancer, breast cancer is the most common cancer diagnosed in women in the United States. Breast cancer can occur in both men and women, but it's far more common in women. Aim of this study to evaluate the lactate dehydrogenase and Gamma glutamyl transpeptidase as screening tests among women with breast cancer. Fifty women diagnosed with breast cancer were involved in this cross-sectional study, they were attended to Khartoum oncology hospital for diagnosis, treatment and follow up. They were grade I, II and III. Approval of study to be performed was obtained from each patient and hospital administration as well. Under hygienic condition blood withdrawn in plane containers from each subject considering labeling and data obtained from each one, blood allowed for clot formation and subsequent serum separation which preserved at -20 ℃ for later chemical analysis. Chemical analysis targeted enzymes, GGT, LDH and ALP, they measured by means of spectrophotometer method, using Biosystem device, reagents were Biosystem trademark, they were ready to use. This study conducted in concern of breast cancer, it involved 50 women with established diagnosis, the Mean±SD duration of the disease was (3.52+1.89) years and their age Mean±SD was (38.1+6.31) year’s .They were enrolled in the measurement of GGT and LDH Comparing the levels of measured parameters with normal value for each one, brought significant difference for LDH and ALP.Enzymes level among the three grades of breast cancer brought significant difference for LDH at grade II and III as p values 0.022 and 0.001 respectively, while GGT has only significant difference at grade III as p value 0.022. Significant elevation in serum levels of lactate dehydrogenase and Gamma glutamyl transpeptidase among patients with breast cancer and suggest that the estimation of both can be used as routine screening tests in all suspected carcinoma breast patients.

العلاقة: https://zenodo.org/communities/gscbps; https://zenodo.org/record/4283215; https://doi.org/10.5281Test/zenodo.4283215; oai:zenodo.org:4283215

الإتاحة: https://doi.org/10.5281Test/zenodo.4283215
https://doi.org/10.30574/gscbps.2019.9.3.0229Test
https://doi.org/10.5281Test/zenodo.4283214
https://zenodo.org/record/4283215