التفاصيل البيبلوغرافية
| العنوان: |
Detection Glutathion Reductase after Electrophoresis on Nagative or Sodium Dodecyl Sulfate Polyarylamide Gels |
| المؤلفون: |
Hou, Wen-Chi, Liang, Hong-Jen, Wang, Ching-Chiung and Liu, Der-Zen |
| المساهمون: |
食品科學系(所) |
| سنة النشر: |
2004 |
| المجموعة: |
YuanPei University Institutional Repository (YPUIR) |
| مصطلحات موضوعية: |
HPLC, peptides, electrochemical detection, glutathione, cyst(e)ine, thiol, disulfide |
| الوصف: |
A determination of glutathione, cysteine, and their disulfides using HPLC and dual electrochemical detection (HPLC-DEC) was described previously but was not validated in biological tissues for these and other important thiols and disulfides (SH/SS). Thus, our objectives were to develop this method to quantify simultaneously reduced and oxidized glutathione, cysteine, cystine, and other SH/SS in various tissues, including human blood and plasma, rat liver and hippocampus, mosquito, and spinach leaf. Optimal conditions were determined for sample processing and analysis using metaphosphoric acid and HPLC-DEC. Authentic standards of 10 common SH/SS compounds were resolved and eluted within 15 min, and all standard curves were linear from 5 to 1600 pmol. Validation was based on the following: First, tissue sample sizes were proportional to peak areas over an eightfold range. Second, recovery of SH/SS added to samples before processing was 96–101%. Finally, the results were equivalent and correlated highly with values for total SH by 5,5′-dithiobis-(2-nitrobenzoic acid) (DTNB) assay (r2 = 0.996) and for total glutathione by DTNB-GSSG reductase assay (r2 = 0.998). The life span of the Au/Hg electrode was limited to 200–500 samples based on the lineal range of standard curves. On the basis of these results, we believe that this method will fill a long-time need for the simultaneous determination of SH/SS in biological tissues. |
| نوع الوثيقة: |
other/unknown material |
| اللغة: |
English |
| العلاقة: |
Electrophoresis Vol. 25 P2926-2931; http://120.106.195.12/handle/310904600Q/1647Test |
| الإتاحة: |
http://120.106.195.12/handle/310904600Q/1647Test |
| رقم الانضمام: |
edsbas.7E2F2A73 |
| قاعدة البيانات: |
BASE |
