دورية أكاديمية
A Role of Rab29 in the Integrity of the Trans-Golgi Network and Retrograde Trafficking of Mannose-6-Phosphate Receptor
| العنوان: | A Role of Rab29 in the Integrity of the Trans-Golgi Network and Retrograde Trafficking of Mannose-6-Phosphate Receptor |
|---|---|
| المؤلفون: | Wang, Shicong, Ma, Zexu, Xu, Xiaohui, Wang, Zhen, Sun, Lixiang, Zhou, Yunhe, Lin, Xiaosi, Hong, Wanjin, Wang, Tuanlao, 洪万进, 王团老 |
| المصدر: | http://dx.doi.org/10.1371/journal.pone.0096242Test. |
| بيانات النشر: | PUBLIC LIBRARY SCIENCE |
| سنة النشر: | 2014 |
| المجموعة: | Xiamen University Institutional Repository |
| مصطلحات موضوعية: | MANNOSE 6-PHOSPHATE RECEPTOR, LYSOSOMAL STORAGE DISEASES, RAB34 INTERACTION, ENDOSOMES, PROTEIN, CARGO, RETROMER, MAINTENANCE, EFFECTOR, CELLS |
| الوصف: | National Natural Science Foundation of China [31071176, 30971442, 30928011]; International S & T Cooperation Program of China [2013DFG32730] ; Rab29 (also referred as Rab7L1) is a novel Rab protein, and is recently demonstrated to regulate phagocytosis and traffic from the Golgi to the lysosome. However, its roles in membrane trafficking have not been investigated extensively. Our results in this study revealed that Rab29 is associated with the trans-Golgi network (TGN), and is essential for maintaining the integrity of the TGN, because inhibition of the activity of Rab29 or depletion of Rab29 resulted in fragmentation of the TGN marked by TGN46. Expression of the dominant negative form Rab29T21N or shRNA-Rab29 also altered the distribution of mannose-6-phosphate receptor (M6PR), and interrupted the retrograde trafficking of M6PR through monitoring the endocytosis of CD8-tagged calcium dependent M6PR (cdM6PR) or calcium independent M6PR (ciM6PR), but without significant effects on the anterograde trafficking of vesicular stomatitis virus G protein (VSV-G). Our results suggest that Rab29 is essential for the integrity of the TGN and participates in the retrograde trafficking of M6PRs. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | PLOS ONE, 2014,9(5):-; WOS:000336655700068; http://dspace.xmu.edu.cn/handle/2288/93077Test |
| الإتاحة: | https://doi.org/10.1371/journal.pone.0096242Test http://dspace.xmu.edu.cn/handle/2288/93077Test |
| رقم الانضمام: | edsbas.2C80BA1 |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |