التفاصيل البيبلوغرافية
| العنوان: |
分离草虾杆状病毒包涵体的一种新方法 ; A new approach to puriFy occlusion bodies of Penaeus monodon-type baculovirus |
| المؤلفون: |
杨丰, 林斌, 徐洵, 陈细法 |
| سنة النشر: |
1995 |
| المجموعة: |
Xiamen University Institutional Repository |
| مصطلحات موضوعية: |
虾病毒, 分离包涵体, PCR鉴定, MBV, Shrip virus, isolation of occlusion bodies, PCR |
| الوصف: |
本文报道了一种纯化草虾杆状病毒（MbV）包涵体的方法。取出受MbV感染的虾肝胰组织，在TESP溶液中（50MMOl/dM3TrIS-HCIPH8.0，50MMOl/dM3EdTA，0.1MOl/dM3nACl，0.5MMOl/dM3PMSf）破碎，差迷离心，再经30%~70%蔗糖浓度梯度高速离心，可得到合包涵体的区带。电镜下观察到较纯的包涵体，经蛋白酶k降解后，PCr扩增，经琼脂糖凝胶电泳可见清晰的dnA区带。 ; A new approach to puriFy occlusion bodies (OB) of Penaeus monodon-type baculovirus(MBV) is described.MBV-inFected hepatoancraFa were removed From the shrimps andhomogenized in a solution of TESP buFFer (50mmol/dm' Tris-HCl pH 8.0, 50mmol/dm3EDTA, 0.1mol/dm3 NaCl, 0.5mmol/dm3 PMSF).PMSF (phenylmethyl solFonyl Flouride) wasused as a proteinase inhibitor protecting OB From degradation.The suspension was clariFiedin diFFerential cantriFugation and the pellet was Further separted in a 30%-- 70% sucrosegradient by high speed centriFugation.Occlusion bodies corresponding to a speciFic band inthe gradient was collected and observed under an electron microscope.AFter digestion byproteinase K, the MBV DNA was ampliFied by PCR and visualized on an agarose gel. ; 厦门市虾病攻关专项基金 |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
Chinese |
| تدمد: |
1000-8160 |
| العلاقة: |
台湾海峡,1995,(1):80-82; TWHX199501013; http://dspace.xmu.edu.cn/handle/2288/123627Test |
| الإتاحة: |
http://dspace.xmu.edu.cn/handle/2288/123627Test |
| رقم الانضمام: |
edsbas.745F8BB6 |
| قاعدة البيانات: |
BASE |
