التفاصيل البيبلوغرافية
| العنوان: |
Substance P induces inositol 1,4,5‐trisphosphate and intracellular free calcium increase in cultured normal human epidermal keratinocytes |
| المؤلفون: |
Koizumi, Hiroko, Yasui, Chikako, Fukaya, Torn, Ueda, Tetsuo, Ohkawara, Akira |
| المصدر: |
Experimental Dermatology ; volume 3, issue 1, page 40-44 ; ISSN 0906-6705 1600-0625 |
| بيانات النشر: |
Wiley |
| سنة النشر: |
1994 |
| المجموعة: |
Wiley Online Library (Open Access Articles via Crossref) |
| الوصف: |
Substance P is a neuropeptide which is present in peripheral C nerve endings and released from them. Free nerve endings of C nerve are present in human epidermis. The effects of substance P on the transmembrane signaling system of pig epidermal sheets were previously reported. In these studies, a small amount of cells other than keratinocytes contaminated the epidermal sheets and the species difference from human was also noticed. Therefore we investigated the effects of substance P on cultured normal human epidermal keratinocytes. Alteration of intracellular free calcium (Ca 2+ ) in single living keratinocytes was studied using an inverted fluorescence microscope and Ca 2+ ‐sensitive dye, Fura 2‐AM. Treatment of normal human epidermal kertinocytes with substance P resulted in an increase in inositol 1,4,5‐trisphosphate and in intracellular Ca 2+ . Substance P inhibited DNA synthesis of the keratinocytes in a dose‐dependent manner. These results are consistent with the view that substance P stimulates phosphatidylinositol‐4,5‐bisphosphate hydrolysis of human keratinocytes, resulting in inositol 1,4,5‐trisphosphate‐Ca 2+ signal. |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
English |
| DOI: |
10.1111/j.1600-0625.1994.tb00264.x |
| الإتاحة: |
https://doi.org/10.1111/j.1600-0625.1994.tb00264.xTest |
| حقوق: |
http://onlinelibrary.wiley.com/termsAndConditions#vorTest |
| رقم الانضمام: |
edsbas.2C72F750 |
| قاعدة البيانات: |
BASE |
