المؤلفون: Pasoglou, Vasiliki, Larbi, Ahmed, Collette, Laurence, Annet, Laurence, Jamar, François, Machiels, Jean‐Pascal, Michoux, Nicolas, Berg, Bruno C. Vande, Tombal, Bertrand, Lecouvet, Frederic E.

المساهمون: Fondation Contre le Cancer

المصدر: The Prostate ; volume 74, issue 5, page 469-477 ; ISSN 0270-4137 1097-0045

الإتاحة: https://doi.org/10.1002/pros.22764Test

المؤلفون: Annet, Laurence, Hermoye, Laurent, Peeters, Frank, Jamar, François, Dehoux, Jean‐Paul, Van Beers, Bernard E.

المصدر: Journal of Magnetic Resonance Imaging ; volume 20, issue 5, page 843-849 ; ISSN 1053-1807 1522-2586

الوصف: Purpose To describe the use of MRI and a cortical‐compartment model to measure the glomerular filtration rate (GFR), and compare the results with those obtained with the Patlak‐Rutland model. Materials and Methods Dynamic MRI of rabbit kidneys was performed during and after injection of gadoterate dimeglumine. The enhancement curves in the aorta and the kidney were analyzed with the cortical‐compartment and Patlak‐Rutland models to assess the GFR. Results A substantial correlation was observed between the GFR measured with MRI using the cortical‐compartment model and the plasma clearance of 51Cr‐EDTA (r = 0.821, P = 0.004). No significant correlation was observed between the 51Cr‐EDTA clearance (r = 0.628, P = 0.052) and the GFR obtained with the Patlak‐Rutland model in regions of interest (ROIs) encompassing the renal cortex and medulla. A Bland and Altman analysis showed that GFR cortical compartment agreed better with the 51Cr‐EDTA clearance compared to GFR Patlak when ROIs were limited to the cortex. However, the GFR values obtained by MRI were lower than the plasma clearance of 51Cr‐EDTA. Conclusion MRI with a cortical‐compartment model provides more accurate assessments of glomerular filtration than the Patlak‐Rutland model. J. Magn. Reson. Imaging 2004;20:843–849. © 2004 Wiley‐Liss, Inc.

الإتاحة: https://doi.org/10.1002/jmri.20173Test

المؤلفون: Chapman, Peter T., Yarwood, Helen, Harrison, Andrew A., Stocker, Claire J., Jamar, François, Gundel, Robert H., Peters, A. Michael, Haskard, Dorian O.

المصدر: Arthritis & Rheumatism ; volume 40, issue 5, page 955-965 ; ISSN 0004-3591 1529-0131

الوصف: Objective. There is relatively little direct evidence for the roles of interleukin‐1 (IL‐1) and tumor necrosis factor α (TNFα) in activating endothelium in vivo. The aim of this study was to use in vitro and in vivo models to investigate the contribution of these cytokines to both E‐selectin expression and the recruitment of polymor‐phonuclear cells (PMN) in monosodium urate monohydrate (MSU) crystal‐induced inflammation. Methods. MSU crystals were incubated with freshly isolated mononuclear cells, after which the harvested supernatants were tested for their ability to induce E‐selectin expression during coculture with human umbilical vein endothelial cells. Subsequent experiments were performed with the addition of neutralizing anticytokine antibodies/antisera. The role of TNFα was then studied in an MSU crystal‐induced monarthritis model, in the presence or absence of anti‐TNFα (5 mg/kg intravenously). 99m technetium ( 99m Tc)‐labeled PMN cells and 111 indium ( 111 In)‐labeled anti‐E‐selectin monoclonal antibody (MAb) 1.2B6 were intravenously administered 4 hours after intraarticular injection to quantify PMN recruitment and E‐selectin expression in inflamed joints. Results. MSU crystals were a potent stimulus for IL‐1 and TNFα production by monocytes in vitro, and these cytokines fully accounted for MSU crystalstimulated, monocyte‐mediated endothelial activation. In the MSU crystal‐induced monarthritis model, TNFα blockade was very effective in suppressing both E‐selectin expression and PMN emigration into the inflamed joints, as judged by gamma‐camera image analysis and postmortem tissue counting following the intravenous injection of 99m Tc‐PMN and 111 In‐anti‐E‐selectin MAb. Conclusion. IL‐1 and TNFα appear to be the only factors released by monocytes following incubation with MSU crystals, which induce E‐selectin expression in vitro. Anti‐TNFα is effective in suppressing endothelial activation and PMN recruitment in vivo E‐selectin imaging can be used to assess the endothelial response to therapy ...

الإتاحة: https://doi.org/10.1002/art.1780400525Test

المؤلفون: Chapman, Peter T., Jamar, François, Harrison, Andrew A., Binns, Richard M., Peters, A. Michael, Haskard, Dorian O.

المصدر: Arthritis & Rheumatism ; volume 37, issue 12, page 1752-1756 ; ISSN 0004-3591 1529-0131

الوصف: Objective . To assess the expression of the cytokine‐inducible endothelial leukocyte adhesion molecule E‐selectin during the evolution of urate crystal–induced arthritis, using a recently described radiolabeled monoclonal antibody (MAb) imaging technique. Methods . Monosodium urate (MSU) crystals and saline alone were injected respectively into the right (inflamed) and left (control) knees of 3 young pigs. Four hours later, 111 In‐labeled 1.2B6 F(ab') 2 (anti–E‐selectin MAb) and 125 I‐labeled MOPC 21 F(ab') 2 (control MAb) were injected intravenously. Uptake of 1.2B6 in inflamed and control joints was assessed by scintigraphy 7 and 24 hours after intraarticular injection of MSU crystals. Immunohistochemistry studies and radioactivity counting of tissues were performed postmortem to confirm the observations from scintigraphy. Results . MAb 1.2B6 F(ab') 2 scintigraphic images of the knees revealed a significantly increased uptake in the right (inflamed) knee at 7 and 24 hours postinjection, particularly over the joint space. These in vivo images were consistent with E‐selectin expression in the inflamed tissue detected by immunohistochemistry and with radioactivity counts postmortem. The synovial localization ratio (inflamed:control synovium counts) was 25.4 ± 9.7 (mean ± SD) for the anti–E‐selectin MAb compared with 2.5 ± 0.9 for the control MAb ( P < 0.05, by paired Student's t ‐test). Conclusion . E‐selectin is expressed by synovial endothelium during the evolution of urate crystal–induced arthritis and can be detected noninvasively using a radiolabeled MAb. This E‐selectin imaging technique has considerable potential for the noninvasive assessment of endothelial activation in arthritis and other inflammatory rheumatic diseases.

الإتاحة: https://doi.org/10.1002/art.1780371207Test

المؤلفون: Chapman, Peter T., Jamar, François, Keelan, Edward T. M., Peters, A. Michael, Haskard, Dorian O.

المصدر: Arthritis & Rheumatism ; volume 39, issue 8, page 1371-1375 ; ISSN 0004-3591 1529-0131

الوصف: Objective . To determine the potential of 111 Inlabeled anti–E‐selectin monoclonal antibody (MAb) to image localized endothelial activation in rheumatoid arthritis (RA). Methods . Fourteen patients with RA were studied after intravenous administration of 111 In‐labeled F(ab′) 2 fragments of MAb against the cytokine‐inducible endothelial cell activation antigen E‐selection (MAb 1.2B6). To compare uptake of 1.2B6 with that of nonspecific immunoglobulin 111 In‐labeled polyclonal human immunoglobulin (HIG) was separately administered to 6 of these patients and the relative uptake of each tracer was determined. Results . Prominent and discrete uptake of the radiolabeled MAb 1.2B6 was clearly visible in inflamed joints of all patients. Compared with 111 In‐HIG, 111 In‐1.2B6 provided superior images in terms of sensitivity and image intensity. Furthermore, the distribution of uptake in inflamed joints was different for the 2 tracers, with 1.2B6 showing a more focal localization in synovium. Conclusion . This study demonstrates that it is possible to objectively assess E‐selectin expression on activated endothelium in vivo in patients with RA, using a radiolabeled MAb. This technique has considerable potential for monitoring disease activity and response to therapy in inflammatory diseases.

الإتاحة: https://doi.org/10.1002/art.1780390815Test

المؤلفون: JAMAR, FRANCOIS, FIELD, CATHERINE, LENERS, NORBERT, FERRANT, AUGUSTIN

المصدر: British Journal of Haematology ; volume 90, issue 1, page 22-30 ; ISSN 0007-1048 1365-2141

الوصف: To specify the validity of bone marrow scanning using a monoclonal anti‐granulocyte antibody labelled with 99m Tc (BW 250/183) for the functional assessment of haemopoiesis, we compared this method with 52 Fe scan in 16 patients with haematological disorders. The examinations were performed using a rectilinear whole‐body scanner and the distribution of the two tracers was assessed visually and quantitatively in anatomical bone marrow segments, the spleen and liver. Qualitative comparison showed concordance in the bone marrow distribution of the two tracers in 83% of the segments. Discrepancies were found in six patients with hypoplastic or aplastic marrow. The spleen was visualized in all cases with the 99m Tc‐Moab, including nine patients without splenic haemopoiesis (i.e. without spleen uptake of 52 Fe). The uptake of the two tracers, quantified in bone marrow segments and the spleen, correlated well (PO‐0001), but not in the liver (NS). The correlation between the uptake values for each patient was excellent, except in cases of aplastic bone marrow. In conclusion, bone marrow scanning using a 99m Tc labelled anti‐granulocyte monoclonal antibody enables functional evaluation of the distribution of haemopoiesis. Limitations include the evaluation of bone marrow aplasia and identification of splenic haemopoiesis, for which 52 Fe remains the tracer of choice.

الإتاحة: https://doi.org/10.1111/j.1365-2141.1995.tb03376.xTest

المؤلفون: Hanin, François‐Xavier, Jamar, François

المصدر: Diagnostic Imaging of Infections and Inflammatory Diseases ; page 273-290 ; ISBN 9781118484418 9781118484388

الإتاحة: https://doi.org/10.1002/9781118484388.ch16Test