المؤلفون: Wójcik, S., Spodnik, J. H., Sidor-Kaczmarek, J., Moryś, J.

المساهمون: This work was supported by a Polish Ministry of Science and Higher Education grant - NN401 005735 (to SW) and by a statutory (ST-11) grant from the Polish Ministry of Science and Higher Education ST-0011/07/211.

المصدر: Folia Morphologica; Vol 74, No 4 (2015); 428-433

مصطلحات موضوعية: epoxomicin, lactacystin, MG-132, synapsin I, rat, neuronal nuclear antigen, Western blot

الوصف: Background: We have recently described changes present in nigrostriatal terminals after intraperitoneal administration of MG-132 and changes that occur in the walls of the rat lateral ventricle after intraventricular administration of MG-132, lactacystin and epoxomicin — different classes of proteasome inhibitors. Substances that inhibit ubiquitin-proteasome system (UPS) activity, are intensively studied due to their potential role as novel therapeutic strategies in the treatment of cancer and ischaemia-reperfusion injury in the brain. The aim of this study is to determine the influence of intraventricular administration of MG-132, lactacystin and epoxomicin on the level in the rat striatum synapsin I — one of the most prominent neuron-specific phosphoproteins in the brain. Materials and methods and Results: Two weeks after administration of studied proteasome inhibitors, substantial reduction (up to 80%) of synapsin I was ob­served in the rat striatum. Because neurons, and especially dopaminergic ones, are sensitive to the depletion of proteasome function, we assume that observed synapsin I decrease may reflect changes in population of striatal neurons and/or nigrostriatal terminals. Conclusions: Understanding of cellular mechanisms standing behind our findings needs further studies, and could provide valuable contribution to the discussion on the mechanisms linking UPS inhibition and survival of neurons.

وصف الملف: application/pdf; application/xhtml+xml

العلاقة: https://journals.viamedica.pl/folia_morphologica/article/view/FM.2015.0103Test

الإتاحة: https://doi.org/10.5603/FM.2015.0103Test
https://journals.viamedica.pl/folia_morphologica/article/view/FM.2015.0103Test

المؤلفون: Badowska-Szalewska, E., Klejbor, I., Sidor-Kaczmarek, J., Cecot, T., Lietzau, G., Spodnik, J.H., Moryś, J.

المصدر: Folia Morphologica; Vol 68, No 3 (2009); 119-128

مصطلحات موضوعية: IL-1β, hippocampus, amygdala, open field, forced swim

الوصف: The aim of this study was to investigate the influence of two periods of life, namely P28 and P360, on the changes in interleukin-1beta (IL-1β) immunoreactivity (-ir) in the hippocampus (CA1, CA3, DG) and amygdala (central-CeA, medial-MeA) caused by acute and repeated open field (OF), or by forced swim (FS) exposition. Rats were divided into groups: non-stressed, exposed to acute (one-time for 15 min) and chronic stressors (21 days for 15 min daily). We found IL-1β-ir in the control group to be higher in P360 than in P28. In P28, under OF and FS exposure, IL-1β-ir in the CeA remained unaltered but increased in the MeA and in the hippocampus after acute and chronic stress. In P360 no changes were observed in the IL-1β-ir level after acute and chronic stimulation. These data demonstrate that only the levels of IL-1β-ir in juvenile rat brains are affected by FS and OF. Additionally, there was no significant difference between FS and OF stimulation in IL-1β-ir.

وصف الملف: application/pdf

العلاقة: https://journals.viamedica.pl/folia_morphologica/article/view/15916Test

الإتاحة: https://journals.viamedica.pl/folia_morphologica/article/view/15916Test

المؤلفون: Sidor-Kaczmarek, J, Labuda, C, Litwinowicz, B, Spodnik, JH, Kowiański, P, Dziewiątkowski, J, Moryś, J

المصدر: Folia Morphologica; Vol 63, No 3 (2004); 285-288

مصطلحات موضوعية: SNAP-25, protein expression, Western blotting, development, cerebral cortex, striatum, rat

الوصف: The developmental changes of 25-kDa synaptosomal-associated protein (SNAP-25) expression in the rat striatum and cerebral cortex were examined using Western- blotting and densitometric scanning of immunoblots. Analysis of the striatum extracts from postnatal day 0 (P0) to postnatal day 120 (P120) demonstrated that SNAP-25 is poorly expressed until P14. From this point the expression level gradually increases to reach a maximum on P60 and then decreases. The pattern of SNAP-25 expression in the rat cerebral cortex is different. Two peaks are observed, the first on P10 and the second on P60, after which the expression level decreases. These results appear to confirm the role of SNAP-25 protein in axon outgrowth and synaptogenesis in the nervous system.

وصف الملف: application/pdf

العلاقة: https://journals.viamedica.pl/folia_morphologica/article/view/16226Test

الإتاحة: https://journals.viamedica.pl/folia_morphologica/article/view/16226Test