دورية أكاديمية
Evaluation or the Gold-Plated Silver Sample Block on the GeneAmp PCR System 9700 Thermal Cycler
| العنوان: | Evaluation or the Gold-Plated Silver Sample Block on the GeneAmp PCR System 9700 Thermal Cycler |
|---|---|
| المؤلفون: | Moore, Melody Ann |
| المساهمون: | Arthur Eisenberg, Joseph Warren, John Planz |
| سنة النشر: | 2019 |
| المجموعة: | UNTHSC Scholarly Repository (University. of North Texas Health Science Center) |
| مصطلحات موضوعية: | Cell and Developmental Biology, Equipment and Supplies, Genetic Processes, Genetics, Genetics and Genomics, Health Information Technology, Investigative Techniques, Life Sciences, Medical Genetics, Medicine and Health Sciences, Molecular Genetics, Other Genetics and Genomics, Gold-plated silver sample block, GeneAmp PCR System, 9700 Thermal Cycler, interchangeable, amplifications, electrophoretic characteristics, allele concordance, peak heights, peak height ratio, software packages, operating systems, interchangeability, Macintosh, Windows NT platforms |
| الوصف: | Moore, Melody Ann. Evaluation of the Gold-plated Silver Sample Block on the GeneAmp PCR System 9700 Thermal Cycler. Master of Science (Forensic Genetics), May 2003, 49 pages, 9 figures, 3 tables, 2 appendices, 24 references. The GeneAmp PRC System 9700 Thermal Cycler has been introduced with interchangeable silver and gold-plated silver sample blocks. To validate the new gold-plated silver sample block on the System 9700, amplifications were performed on both the gold-plated silver and the previously validated silver sample blocks. PCR amplifications using the AmpFLSTR Profiler Plus ID COfiler, Identifiler and SGM Plus typing kits (Applied Biosystems, Forster City, CA) were performed. Electrophoretic characteristics such as allele concordance, peak heights, and peak height ratios were used to discern any differences in the amplification capabilities of the two sample blocks. The results demonstrate that the PCR reactions on both silver and gold-plated silver blocks are equivalent. In addition, the data obtained (allele cells, peak heights, peak height ratios) from both Macintosh and Windows NT platforms were identical for each amplification kit, indicating concordance between the software packages of each operating system. The results of this study demonstrate the interchangeability of the silver and gold-plated sample blocks. |
| نوع الوثيقة: | text |
| وصف الملف: | application/pdf |
| اللغة: | English |
| العلاقة: | https://hdl.handle.net/20.500.12503/29526Test |
| الإتاحة: | https://doi.org/20.500.12503/29526Test https://hdl.handle.net/20.500.12503/29526Test |
| رقم الانضمام: | edsbas.1E1BA135 |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |