دورية أكاديمية
Breg Cells Are Numerically Decreased and Functionally Impaired in Patients with Systemic Sclerosis
| العنوان: | Breg Cells Are Numerically Decreased and Functionally Impaired in Patients with Systemic Sclerosis |
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| المؤلفون: | Mavropoulos A., Simopoulou T., Varna A., Liaskos C., Katsiari C.G., Bogdanos D.P., Sakkas L.I. |
| المصدر: | Arthritis and Rheumatology ; https://www.scopus.com/inward/record.uri?eid=2-s2.0-84956737145&doi=10.1002%2fart.39437&partnerID=40&md5=5ee3666b65d6c5a69afcf5923d5a55bbTest |
| سنة النشر: | 2016 |
| المجموعة: | University of Thessaly Institutional Repository / Ιδρυματικό Αποθετήριο Πανεπιστημίου Θεσσαλίας |
| مصطلحات موضوعية: | CD19 antigen, CD24 antigen, CD27 antigen, CD38 antigen, IL10 protein, human, interleukin 10, lymphocyte antigen receptor, mitogen activated protein kinase p38, STAT3 protein, toll like receptor 9, adult, aged, B lymphocyte, case control study, complication, female, flow cytometry, immunology, lymphocyte activation, lymphocyte count, male, middle aged, Pulmonary Fibrosis, rheumatoid arthritis, severity of illness index, systemic sclerosis, Antigens, CD19, CD24 |
| الوصف: | Objective Breg cells, a regulatory cell subset that produces interleukin-10 (IL-10), play a significant role in suppressing autoimmune responses and preventing autoimmunity. This study was undertaken to examine the number and function of Breg cells in patients with systemic sclerosis (SSc), a disease with many autoantibodies. Methods Forty-five patients with SSc (12 with early SSc, 33 with established disease including 16 with SSc-associated pulmonary fibrosis [PF]), 12 healthy control subjects, and 10 patients with rheumatoid arthritis (RA)-associated PF were studied. The phenotypes of immature/transitional Breg cells (CD19+CD24highCD38high) and memory Breg cells (CD19+CD27+CD24high) were evaluated by flow cytometry. The function of Breg cells was assessed by measuring the production of IL-10 after B cell activation. In addition, activation of p38 MAPK and STAT-3 was measured following stimulation of the cells with B cell receptor (BCR) and Toll-like receptor 9 (TLR-9). Results Percentages of memory Breg cells were decreased in patients with early SSc (mean ± SEM 1.85 ± 0.38%), those with established SSc (1.6 ± 0.88%), those with SSc-associated PF (1.52 ± 0.17%), and those with RA-associated PF (1.58 ± 0.26%), compared to healthy controls (6.3 ± 0.49%; each P < 0.001). Percentages of transitional Breg cells were also decreased. Expression of IL-10 by Breg cells after stimulation with TLR-9 was impaired in patients with SSc, particularly those with SSc-associated PF. Activation of STAT-3 and p38 MAPK was impaired in naive and memory B cells from patients with SSc after stimulation with BCR and TLR-9. Expression of the stimulatory CD19 receptor was increased in B cells and also increased, to a lesser extent, in Breg cells from patients with SSc compared to healthy controls. Percentages of memory B cells were decreased in patients with SSc, particularly in those with SSc-associated PF. Conclusion This is the first study to demonstrate that Breg cells are phenotypically and functionally impaired in patients with ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 23265191 |
| العلاقة: | http://hdl.handle.net/11615/76444Test |
| DOI: | 10.1002/art.39437 |
| الإتاحة: | https://doi.org/10.1002/art.39437Test http://hdl.handle.net/11615/76444Test |
| رقم الانضمام: | edsbas.725B545A |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 23265191 |
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| DOI: | 10.1002/art.39437 |