دورية أكاديمية
Epithelial-to-mesenchymal transition heterogeneity of circulating tumor cells and their correlation with mdscs and tregs in HER2-negative metastatic breast cancer patients
| العنوان: | Epithelial-to-mesenchymal transition heterogeneity of circulating tumor cells and their correlation with mdscs and tregs in HER2-negative metastatic breast cancer patients |
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| المؤلفون: | Papadaki M.A., Aggouraki D., Vetsika E.-K., Xenidis N., Kallergi G., Kotsakis A., Georgoulias V. |
| المصدر: | Anticancer Research ; https://www.scopus.com/inward/record.uri?eid=2-s2.0-85100683779&doi=10.21873%2fANTICANRES.14817&partnerID=40&md5=d4f3dc1c6a8f68e1f22e6e0c6de38127Test |
| سنة النشر: | 2021 |
| المجموعة: | University of Thessaly Institutional Repository / Ιδρυματικό Αποθετήριο Πανεπιστημίου Θεσσαλίας |
| مصطلحات موضوعية: | aldehyde dehydrogenase isoenzyme 1, antineoplastic agent, CD11b antigen, CD14 antigen, CD15 antigen, CD3 antigen, CD33 antigen, CD4 antigen, epidermal growth factor receptor 2, HLA DR antigen, inducible nitric oxide synthase, reactive oxygen metabolite, transcription factor Twist, Twist related protein 1, tumor marker, adult, aged, Article, breast carcinoma, cancer chemotherapy, cancer hormone therapy, CD25+ T lymphocyte, CD3+ T lymphocyte, CD4+ T lymphocyte, cell heterogeneity, cell subpopulation, circulating tumor cell, clinical article, controlled study, epithelial mesenchymal transition |
| الوصف: | Background: To investigate the correlation between circulating tumor cells (CTCs) bearing cancer stem cell (CSC) and epithelial-to-mesenchymal (EMT) phenotypes and the different immunosuppressive cells in peripheral blood of patients with metastatic breast cancer (mBC). Materials and Methods: Blood was obtained from 38 pre-treated patients with mBC before a new line of treatment. CTC detection and characterization was performed by triple immunofluorescent staining, while Myeloid-derived Suppressor Cells (MDSCs) and T regulatory cells (Tregs) were analyzed by multi-flow cytometry. Results: CTCs were detected in 16 (42.1%) of patients. Based on the co-expression of ALDH1, TWIST and CK, CTCs revealed an important heterogeneity: CTCs with a CSC/partial-EMT, CSC/Epithelial-like, non-CSC/partial-EMT and non-CSC/Epithelial-like phenotype were detected in 7 (18.4%), 7 (18.4%), 1 (1.4%) and 9 (23.7%) of patients, respectively. Immunophenotyping of MDSCs identified 2 monocytic [M-MDSCs; CD14+CD15+CD11b+CD33+HLADR- Lin- (CD14+CD15+) and CD14+CD15-CD11b+CD33+HLA-DR-Lin- (CD14+CD15-)] and one granulocytic [GMDSCs; CD14-CD15+CD11b+CD33+HLA-DR-Lin- (CD14- CD15+)] subpopulations, expressing inducible nitric oxide synthase (iNOS) and reactive oxygen species (ROS), respectively. Patients with detectable CTCs had a higher frequency of Tregs (CD3+CD4+CD25high; p=0.022) whereas a positive correlation was found between CTC counts and the percentage of Tregs (p=0.005) and CD14+CD15+M-MDSCs (p=0.024). Patients with a partial-EMT phenotype had a higher frequency of CD14+CD15+M-MDSCs (p=0.023). Patients harboring the non-CSC/epithelial-like CTC subpopulation had an increased frequency of CD14-CD15+GMDSCs (p=0.020), along with decreased levels of CD3+CD4+CD25high FoxP3+Tregs (p=0.020). Conclusion: These findings provide evidence that CTCs in ER+/HER2- mBC patients may be under the control of the immune system and various immune escape mechanisms might be involved during the different stages of their biological evolution. © 2021 ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 02507005 |
| العلاقة: | http://hdl.handle.net/11615/77535Test |
| DOI: | 10.21873/ANTICANRES.14817 |
| الإتاحة: | https://doi.org/10.21873/ANTICANRES.14817Test http://hdl.handle.net/11615/77535Test |
| رقم الانضمام: | edsbas.F363FF59 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 02507005 |
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| DOI: | 10.21873/ANTICANRES.14817 |