دورية أكاديمية

Sputum Proteome Signatures of Mechanically Ventilated Intensive Care Unit Patients Distinguish Samples with or without Anti-pneumococcal Activity

التفاصيل البيبلوغرافية
العنوان: Sputum Proteome Signatures of Mechanically Ventilated Intensive Care Unit Patients Distinguish Samples with or without Anti-pneumococcal Activity
المؤلفون: Seinen, Jolien, Engelke, Rudolf, Abdullah, Mohammed R, Voß, Franziska, Michalik, Stephan, Dhople, Vishnu M, Dieperink, Willem, de Smet, Anne Marie G A, Völker, Uwe, van Dijl, Jan Maarten, Schmidt, Frank, Hammerschmidt, Sven
المصدر: Seinen , J , Engelke , R , Abdullah , M R , Voß , F , Michalik , S , Dhople , V M , Dieperink , W , de Smet , A M G A , Völker , U , van Dijl , J M , Schmidt , F & Hammerschmidt , S 2021 , ' Sputum Proteome Signatures of Mechanically Ventilated Intensive Care Unit Patients Distinguish Samples with or without Anti-pneumococcal Activity ' , Msystems , vol. 6 , no. 2 , e00702-20 . https://doi.org/10.1128/mSystems.00702-20Test
سنة النشر: 2021
المجموعة: University of Groningen research database
مصطلحات موضوعية: Streptococcus pneumoniae, mechanical ventilation, sputum, antimicrobial peptides, immunoproteomics, proteomics
الوصف: Mechanically ventilated patients are at risk of contracting pneumonia. Therefore, these patients often receive prophylactic systemic antimicrobial therapy. Intriguingly however, a previous study showed that antimicrobial activity in bronchoalveolar aspirates (here referred to as "sputa") from ventilated patients was only partially explained by antibiotic therapy. Here we report that sputa from these patients presented distinct proteome signatures depending on the presence or absence of antimicrobial activity. Moreover, we show that the same distinction applied to antibodies against Streptococcus pneumoniae, which is a major causative agent of pneumonia. Specifically, the investigated sputa that inhibited growth of S. pneumoniae, while containing subinhibitory levels of the antibiotic cefotaxime, presented elevated levels of proteins implicated in innate immune defenses, including complement and apolipoprotein-associated proteins. In contrast, S. pneumoniae-inhibiting sputa with relatively high cefotaxime concentrations or noninhibiting sputa contained higher levels of proteins involved in inflammatory responses, such as neutrophil elastase-associated proteins. In an immunoproteomics analysis, 18 out of 55 S. pneumoniae antigens tested showed significantly increased levels of IgGs in inhibiting sputa. Hence, proteomics and immunoproteomics revealed elevated levels of antimicrobial host proteins or S. pneumoniae antigen-specific IgGs in pneumococcal growth-inhibiting sputa, thus explaining their anti-pneumococcal activity. IMPORTANCE Respiratory pathogens like Streptococcus pneumoniae can cause severe pneumonia. Nonetheless, mechanically ventilated intensive care patients, who have a high risk of contracting pneumonia, rarely develop pneumococcal pneumonia. This suggests the presence of potentially protective antimicrobial agents in their lung environment. Our present study shows for the first time that bronchoalveolar aspirates, "sputa," of ventilated patients in a Dutch intensive care unit were characterized by ...
نوع الوثيقة: article in journal/newspaper
وصف الملف: application/pdf
اللغة: English
العلاقة: https://research.rug.nl/en/publications/ea91fd4e-e82d-49e6-9ff5-70a8a61fc9a5Test
DOI: 10.1128/mSystems.00702-20
الإتاحة: https://doi.org/10.1128/mSystems.00702-20Test
https://hdl.handle.net/11370/ea91fd4e-e82d-49e6-9ff5-70a8a61fc9a5Test
https://research.rug.nl/en/publications/ea91fd4e-e82d-49e6-9ff5-70a8a61fc9a5Test
https://pure.rug.nl/ws/files/163988654/Sputum_Proteome_Signatures_of_Mechanically_Ventilated_Intensive_Care_Unit_Patients_Distinguish_Samples_with_or_without_Anti_pneumococcal_Activity.pdfTest
حقوق: info:eu-repo/semantics/openAccess
رقم الانضمام: edsbas.5525A8CD
قاعدة البيانات: BASE