دورية أكاديمية
MCPIP1 contributes to clear cell renal cell carcinomas development.
| العنوان: | MCPIP1 contributes to clear cell renal cell carcinomas development. |
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| المؤلفون: | Ligeza, Janusz, Marona, Paulina, Gach, Natalia, Lipert, Barbara, Miekus, Katarzyna, Wilk, Waclaw, Jaszczynski, Janusz, Stelmach, Andrzej, Loboda, Agnieszka, Dulak, Jozef, Branicki, Wojciech, Rys, Janusz, Jura, Jolanta |
| سنة النشر: | 2017 |
| المجموعة: | University of Auckland Research Repository - ResearchSpace |
| مصطلحات موضوعية: | Cell Line, Tumor, Humans, Carcinoma, Renal Cell, Kidney Neoplasms, Ribonucleases, p38 Mitogen-Activated Protein Kinases, Vascular Endothelial Growth Factor A, Leupeptins, NF-kappa B, Transcription Factors, RNA, Messenger, Signal Transduction, Cell Hypoxia, Cell Survival, Adult, Aged, 80 and over, Middle Aged, Female, Male, Glucose Transporter Type 1, Basic Helix-Loop-Helix Transcription Factors, Proteasome Inhibitors, Carcinogenesis |
| الوصف: | Monocyte Chemoattractant protein-induced protein 1 (MCPIP1), also known as Regnase-1, is encoded by the ZC3H12a gene, and it mediates inflammatory processes by regulating the stability of transcripts coding for proinflammatory cytokines and controlling activity of transcription factors, such as NF-κB and AP1. We found that MCPIP1 transcript and protein levels are strongly downregulated in clear cell renal cell carcinoma (ccRCC) samples, which were derived from patients surgically treated for renal cancer compared to surrounded normal tissues. Using Caki-1 cells as a model, we analyzed the role of MCPIP1 in cancer development. We showed that MCPIP1 expression depends on the proteasome activity; however, hypoxia and hypoxia inducible factor 2 alfa (HIF2α) are key factors lowering MCPIP1 expression. Furthermore, we found that MCPIP1 negatively regulates HIF1α and HIF2α levels and in the case of the last one, the mechanism is based on the regulation of the half time of transcript coding for HIF2α. Enhanced expression of MCPIP1 in Caki-1 cells results in a downregulation of transcripts encoding VEGFA, GLUT1, and IL-6. Furthermore, MCPIP1 decreases the activity of mTOR and protein kinase B (Akt) in normoxic conditions. Taken together, MCPIP1 contributes to the ccRCC development. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | Print-Electronic; application/pdf |
| اللغة: | English |
| تدمد: | 0969-6970 1573-7209 |
| العلاقة: | Angiogenesis; http://hdl.handle.net/2292/44876Test |
| DOI: | 10.1007/s10456-017-9540-2 |
| الإتاحة: | https://doi.org/10.1007/s10456-017-9540-2Test http://hdl.handle.net/2292/44876Test |
| حقوق: | Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. ; https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htmTest ; Copyright: The author ; http://purl.org/eprint/accessRights/RestrictedAccessTest |
| رقم الانضمام: | edsbas.AE5B37EF |
| قاعدة البيانات: | BASE |
| تدمد: | 09696970 15737209 |
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| DOI: | 10.1007/s10456-017-9540-2 |