دورية أكاديمية
Glycogen synthase kinase 3-β inhibition induces lymphangiogenesis through β-catenin-dependent and mTOR-independent pathways.
| العنوان: | Glycogen synthase kinase 3-β inhibition induces lymphangiogenesis through β-catenin-dependent and mTOR-independent pathways. |
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| المؤلفون: | Stump, Benjamin, Shrestha, Shikshya, Lamattina, Anthony M, Louis, Pierce H, Cho, Woohyun, Perrella, Mark A, Ai, Xingbin, Rosas, Ivan O, Wagner, Florence F, Priolo, Carmen, Astin, Jonathan, El-Chemaly, Souheil |
| سنة النشر: | 2019 |
| المجموعة: | University of Auckland Research Repository - ResearchSpace |
| مصطلحات موضوعية: | Lung, Cell Line, Endothelial Cells, Lymphatic Vessels, Humans, Maleimides, Indoles, RNA, Small Interfering, Cell Culture Techniques, Signal Transduction, Phosphorylation, Lymphangiogenesis, beta Catenin, Protein Stability, Microvessels, TOR Serine-Threonine Kinases, Glycogen Synthase Kinase 3 beta |
| الوصف: | Lymphatic vessels play an important role in health and in disease. In this study, we evaluated the effects of GSK3-β inhibition on lung lymphatic endothelial cells in vitro. Pharmacological inhibition and silencing of GSK3-β resulted in increased lymphangiogenesis of lung lymphatic endothelial cells. To investigate mechanisms of GSK3-β-mediated lymphangiogenesis, we interrogated the mammalian/mechanistic target of rapamycin pathway and found that inhibition of GSK3-β resulted in PTEN activation and subsequent decreased activation of AKT, leading to decreased p-P70S6kinase levels, indicating inhibition of the mTOR pathway. In addition, consistent with a negative role of GSK3-β in β-catenin stability through protein phosphorylation, we found that GSK3-β inhibition resulted in an increase in β-catenin levels. Simultaneous silencing of β-catenin and inhibition of GSK3-β demonstrated that β-catenin is required for GSK3-β-induced lymphangiogenesis. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | Electronic-eCollection; application/pdf |
| اللغة: | English |
| تدمد: | 1932-6203 |
| العلاقة: | PloS one; PloS one 14(4):e0213831 Jan 2019; http://hdl.handle.net/2292/50352Test |
| DOI: | 10.1371/journal.pone.0213831 |
| الإتاحة: | https://doi.org/10.1371/journal.pone.0213831Test http://hdl.handle.net/2292/50352Test |
| حقوق: | Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. ; https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htmTest ; https://creativecommons.org/licenses/by/4.0Test/ ; Copyright: The authors ; http://purl.org/eprint/accessRights/OpenAccessTest |
| رقم الانضمام: | edsbas.2557C072 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 19326203 |
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| DOI: | 10.1371/journal.pone.0213831 |