التفاصيل البيبلوغرافية
| العنوان: |
Development of a successful method for quantifying viable oral anaerobic spirochetes from pure culture and periodontal pockets |
| المؤلفون: |
Qiu, Yu-Shan |
| المساهمون: |
Chan, Z. C. S. (advisor) |
| بيانات النشر: |
McGill University |
| سنة النشر: |
1993 |
| المجموعة: |
Theses Canada / Thèses Canada (Library and Archives Canada) |
| مصطلحات موضوعية: |
Biology, Microbiology, Health Sciences, Dentistry |
| الوقت: |
Master of Science (Department of Microbiology and Immunology.) |
| الوصف: |
Spirochetes are markedly prevalent in periodontal disease but are not included as predominant cultivable organisms because of the inability to quantify them by viable count. A successful method was developed for enumerating viable oral spirochetes in pure culture as colony-forming units (CFU) in new oral spirochete (NOS) medium with 0.7% agarose and using small tissue-culture flasks. Three species of oral spirochetes in log-phase growth in NOS broth were used for evaluation of the method. Reliable, consistent and reproducible viable counts of pure spirochete cultures were obtained. ; This method was extended to enumerate viable oral spirochetes from periodontal pockets. The antibiotic rifampin (20 $ mu$g/ml) was found to be an excellent selective agent for such a count when added to NOS-agarose medium. Counts of cultivable oral spirochetes from 10 subgingival plaque samples ranged from 12.5% to 28.2% of the total cultivable anaerobic bacteria. In addition, by the use of this method thirteen new oral spirochetes were isolated. ; The viable count technique was modified and employed to study the locomotion of spirochetes. Migration of oral spirochetes out of NOS-Bacto agar medium into NOS-agarose medium was observed and two locomotory phenotypes of oral spirochetes were detected. |
| نوع الوثيقة: |
thesis |
| وصف الملف: |
application/pdf |
| اللغة: |
English |
| العلاقة: |
alephsysno: 001397531; proquestno: AAIMM94504; Theses scanned by UMI/ProQuest.; http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=68246Test |
| الإتاحة: |
http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=68246Test |
| حقوق: |
All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| رقم الانضمام: |
edsbas.342CAB43 |
| قاعدة البيانات: |
BASE |
