دورية أكاديمية
Abnormally located SSEA1+/SOX9+ endometrial epithelial cells with a basalis-like phenotype in the eutopic functionalis layer may play a role in the pathogenesis of endometriosis
| العنوان: | Abnormally located SSEA1+/SOX9+ endometrial epithelial cells with a basalis-like phenotype in the eutopic functionalis layer may play a role in the pathogenesis of endometriosis |
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| المؤلفون: | Hapangama, DK, Drury, J, Da Silva, L, Al-Lamee, H, Earp, A, Valentijn, AJ, Edirisinghe, DP, Murray, PA, Fazleabas, AT, Gargett, CE |
| بيانات النشر: | Oxford University Press |
| سنة النشر: | 2019 |
| المجموعة: | The University of Liverpool Repository |
| الوصف: | STUDY QUESTION:Is endometriosis associated with abnormally located endometrial basalis-like (SSEA1+/SOX9+) cells in the secretory phase functionalis and could they contribute to ectopic endometriotic lesion formation? SUMMARY ANSWER:Women with endometriosis had an abnormally higher number of basalis-like SSEA1+/SOX9+ epithelial cells present in the stratum functionalis and, since these cells formed 3D structures in vitro with phenotypic similarities to ectopic endometriotic lesions, they may generate ectopic lesions following retrograde menstruation. WHAT IS KNOWN ALREADY:Endometrial basalis cells with progenitor potential are postulated to play a role in the pathogenesis of endometriosis and SSEA1 and nuclear SOX9 (nSOX9) mark basalis epithelial cells that also have some adenogenic properties in vitro. Induction of ectopic endometriotic lesions in a baboon model of endometriosis produces characteristic changes in the eutopic endometrium. Retrograde menstruation of endometrial basalis cells is proposed to play a role in the pathogenesis of endometriosis. STUDY DESIGN, SIZE, DURATION:This prospective study included endometrial samples from 102 women with and without endometriosis undergoing gynaecological surgery and from six baboons before and after induction of endometriosis, with in vitro assays examining the differentiation potential of human basalis-like cells. PARTICIPANTS/MATERIALS, SETTING, METHODS:The study was conducted at a University Research Institute. SSEA1 and SOX9 expression levels were examined in human endometrial samples from women aged 18-55 years (by immunohistochemistry (IHC) and qPCR) and from baboons (IHC). The differential gene expression and differentiation potential was assessed in freshly isolated SSEA1+ endometrial epithelial cells from women with and without endometriosis (n = 8/group) in vitro. In silico analysis of selected published microarray datasets identified differential regulation of genes of interest for the mid-secretory phase endometrium of women with endometriosis ... |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | text |
| اللغة: | English |
| العلاقة: | http://livrepository.liverpool.ac.uk/3029635/1/dey336%20DH.pdfTest; Hapangama, DK orcid:0000-0003-0270-0150 , Drury, J orcid:0000-0002-2275-6664 , Da Silva, L, Al-Lamee, H, Earp, A, Valentijn, AJ, Edirisinghe, DP, Murray, PA, Fazleabas, AT and Gargett, CE (2019) Abnormally located SSEA1+/SOX9+ endometrial epithelial cells with a basalis-like phenotype in the eutopic functionalis layer may play a role in the pathogenesis of endometriosis. Human Reproduction, 34 (1). pp. 56-68. |
| الإتاحة: | http://livrepository.liverpool.ac.uk/3029635Test/ https://academic.oup.com/humrep/article/34/1/56/5213996Test http://livrepository.liverpool.ac.uk/3029635/1/dey336%20DH.pdfTest |
| رقم الانضمام: | edsbas.5BA776F |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |