التفاصيل البيبلوغرافية
| العنوان: |
Subunit fusion of two yeast D-amino acid oxidases enhances their thermostability and resistance to H2O2 |
| المؤلفون: |
Wang, Shih-juei, Yu, Chi-yang, Lee, Cheng-kang, Chern, Ming-kai, Kuan, I-ching |
| المساهمون: |
淡江大學生命科學研究所 |
| بيانات النشر: |
Springer Netherlands |
| سنة النشر: |
2008 |
| المجموعة: |
Tamkang University Institutional Repository (TKUIR) / 淡江大學機構典藏 |
| مصطلحات موضوعية: |
D-Amino acid oxidase, Gene fusion, Rhodosporidium toruloides, Stability, Trigonopsis variabilis |
| الوصف: |
D-Amino acid oxidases from Rhodosporidium toruloides and Trigonopsis variabilis (RtDAO and TvDAO) are both yeast homodimeric flavoenzymes. Two of their cDNA genes were connected by a hexanucleotide linker and heterologously expressed in E. coli to produce the corresponding double DAOs (dRtDAO and dTvDAO) with two subunits fused into a single polypeptide. The specific activities of double DAOs remained similar to those of native dimeric DAOs, although the catalytic efficiencies (kcat/KM) were decreased due to higher KM values. The Tm value for dRtDAO was shifted 5°C higher while that for dTvDAO was increased only by 2°C, in comparison with the corresponding native counterparts. In the presence of 10 mM H2O2, dRtDAO and dTvDAO exhibited half-lives of about 60 and 40 min, respectively, which were 2- and 1.5-fold, respectively, longer than their native DAOs. These yeast DAOs can therefore be thermally and oxidatively stabilized by linking their subunits together. ; 國外 ; SCI ; 紙本 ; 電子版 ; NLD |
| نوع الوثيقة: |
other/unknown material |
| اللغة: |
English |
| العلاقة: |
Biotechnology Letters 30(8), pp.1415-1422; 0141-5492; 1573-6776; http://tkuir.lib.tku.edu.tw:8080/dspace/handle/987654321/61895Test; http://tkuir.lib.tku.edu.tw:8080/dspace/bitstream/987654321/61895/1/index.htmlTest |
| الإتاحة: |
http://tkuir.lib.tku.edu.tw:8080/dspace/handle/987654321/61895Test
http://tkuir.lib.tku.edu.tw:8080/dspace/bitstream/987654321/61895/1/index.htmlTest |
| رقم الانضمام: |
edsbas.672D2A25 |
| قاعدة البيانات: |
BASE |
