دورية أكاديمية
Molecular Basis of Loss-of-Function Mutations in the glp-1 Gene of Caenorhabitis elegans
| العنوان: | Molecular Basis of Loss-of-Function Mutations in the glp-1 Gene of Caenorhabitis elegans |
|---|---|
| المؤلفون: | Kodoyianni, Voula, Maine, Eleanor M, Kimble, Judith |
| المصدر: | Biology - All Scholarship |
| بيانات النشر: | SURFACE at Syracuse University |
| سنة النشر: | 1992 |
| المجموعة: | Syracuse University Research Facility And Collaborative Environment (SUrface) |
| مصطلحات موضوعية: | Mutation, Caenorhabditis elegans, Multigene family, Gene organization, Phenotype, Biology |
| الوصف: | The glp-1 gene encodes a membrane protein required for inductive cell interactions during development of the nematode Caenorhabditis elegans. Here we report the molecular characterization of 15 loss-of-function (lf) mutations of glp-1. Two nonsense mutations appear to eliminate glp-1 activity; both truncate the glp-1 protein in its extracellular domain and have a strong loss-of-function phenotype. Twelve missense mutations and one in-frame deletion map to sites within the repeated motifs of the glp-1 protein (10 epidermal growth factor [EFG]-like and 3 LNG repeats extracellularly and 6 cdc10/SW16, or ankyrin, repeats intracellularly). We find that all three types of repeated motifs are critical to glp-1 function, and two individual EFG-like repeats may have distinct functions. Intriguingly, all four missense mutations in one phenotypic class map to the N-terminal EGF-like repeats and all six missense mutations in a second phenotypic class reside in the intracellular cdc10/SW16 repeats. These two clusters of mutations may identify functional domains within the glp-1 protein. |
| نوع الوثيقة: | text |
| وصف الملف: | application/pdf |
| اللغة: | English |
| العلاقة: | https://surface.syr.edu/bio/15Test; https://surface.syr.edu/context/bio/article/1015/viewcontent/Kodoyianni_et_al_92.pdfTest |
| الإتاحة: | https://surface.syr.edu/bio/15Test https://surface.syr.edu/context/bio/article/1015/viewcontent/Kodoyianni_et_al_92.pdfTest |
| حقوق: | http://creativecommons.org/licenses/by/3.0Test/ |
| رقم الانضمام: | edsbas.A20EC22A |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |