دورية أكاديمية
Determination of Urinary Total Hydroxyproline by HPLC with UV and EC Detectors
| العنوان: | Determination of Urinary Total Hydroxyproline by HPLC with UV and EC Detectors |
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| المؤلفون: | Kaya, Betul Isiner, Dural, Emrah, Kenduzler, Erdal, Soylemezoglu, Tulin |
| المساهمون: | Kaya, Betul Isiner -- Kenduzler, Erdal Burdur Mehmet Akif Ersoy Univ, Fac Arts & Sci, Dept Chem, TR-15030 Burdur, Turkey -- Dural, Emrah Sivas Cumhuriyet Univ, Fac Pharm, Dept Pharmaceut Toxicol, TR-58140 Sivas, Turkey -- Soylemezoglu, Tulin Ankara Univ, Inst Forens Sci, Dept Forens Chem & Forens Toxicol, TR-06590 Ankara, Turkey |
| بيانات النشر: | CHEM SOC PAKISTAN |
| سنة النشر: | 2018 |
| المجموعة: | Sivas Cumhuriyet University Institutional Repository |
| مصطلحات موضوعية: | Hydroxyproline, Tobacco smoke, Urine, HPLC, Ultraviolet detection, Electrochemical detection |
| الوصف: | WOS: 000444687300005 ; Hydroxyproline is found in high concentrations in connective tissue proteins. It is remarkably useful to detect it because variation in urine levels of hydroxyproline is associated with various diseases. High performance liquid chromatography methods, in which ultraviolet and electrochemical detectors were used, were developed and validated for the determination of hydroxyproline in urine. Both methods included acid hydrolysis and derivation. The most appropriate time, temperature and pH were identified to optimize derivatization processes. Limits of detection were calculated as 1.57 mu g/mL, 0.9 mu g/mL and limits of quantification were 4.76 mu g/mL, 2.73 mu g/mL for UVD and ECD methods, respectively. Precision and accuracy of the methods were obtained <= 10.9 % (RSD), <= 11.5% (RE). The recoveries were found 108 +/- 6% and 102 +/- 7% for UVD and ECD methods, respectively. A strong, positive and linear correlation was found between hydroxyproline values obtained from UVD and ECD methods (p<0.01, r=+0.98). After optimization and validation of these methods, OHP levels in urine samples which were obtained from non-smokers and smokers were analyzed and, observed OHP/creatinine levels were compared. Results of both methods showed that total urinary OHP values of smokers were significantly higher than non-smokers (p<0.05). ; Scientific Research Projects Support Program of Burdur Mehmet Akif Ersoy University [0146-YL-11] ; This research was supported by Scientific Research Projects Support Program of Burdur Mehmet Akif Ersoy University under the project number of 0146-YL-11. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 0253-5106 |
| العلاقة: | JOURNAL OF THE CHEMICAL SOCIETY OF PAKISTAN; Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı; https://hdl.handle.net/20.500.12418/6129Test; 40; 875; 866 |
| الإتاحة: | https://doi.org/20.500.12418/6129Test https://hdl.handle.net/20.500.12418/6129Test |
| حقوق: | info:eu-repo/semantics/closedAccess |
| رقم الانضمام: | edsbas.39FFB676 |
| قاعدة البيانات: | BASE |
| تدمد: | 02535106 |
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