التفاصيل البيبلوغرافية
| العنوان: |
ヒト骨中γ-Carboxyglutamic Acid含有蛋白に関する研究 : 蛋白の精製, 抗体の作製, 及び免疫組織化学的研究 ; Human Bone γ-Carboxyglutamic Acid-Containing Protein : Purification, Production of the Specific Antibody and Immunohistochemical Localization in the Human Ossifying Front |
| المؤلفون: |
松山, 敏勝, 小川, 勝洋 |
| بيانات النشر: |
札幌医科大学医学部 |
| سنة النشر: |
1985 |
| المجموعة: |
SIKOR - Sapporo Medical University Information and Knowledge Repository / 札幌医科大学学術機関リポジトリ |
| مصطلحات موضوعية: |
γ-Carboxyglutamic acid, Boneγ-carboxyglutamic acid-containing protein, BGP, Human cortical bone, Calcium-binding protein |
| الوصف: |
Bone γ-carboxyglutamic acid-containing protein （BGP） has an ability to bind calcium ion, being a major component of noncollagenous protein in bone tissue. Although BGP has been isolated from various species, purification of BGP from the human bone by Poser et al. was only successful by means of the immunoadsorbent method. It is a major difficulty to isolate human BGP by the procedures available for other species （extraction with 0.5 M EDTA, pH 8.0, Sephadex G-100 gel filtration, and DEAE Sephadex A-25 ion exchange chromatography） because of degradation of proteins by proteases during prolonged decalcification. We succeeded in the isolation of human BGP by the use of a protease inhibitor. Molecular weight of the purified human BGP was about 8,500-9,000. It showed an altered electrophoretic mobility in the presence of calcium ion. Amino acid analysis revealed that one molecule contains 2 γ-carboxyglutamic acid residues. These physio-chemical properties were consistent with those of BGP, previously isolated from other species. Antisera raised in rabbits to the human BGP reacted to the solid phase fixed BGP even at the dilution of 1/27,000 in ELISA. Immunohistochemical study demonstrated that BGP was localized in the cytoplasm of osteoblasts, osteocytes, hypertrophic chondrocytes and osteoclasts, and the periphery of osteoid matrix in the ossifying front of human bone tissue. These findings support the hypothesis that BGP has a major role in the ossification of bone tissue. ; departmental bulletin paper |
| نوع الوثيقة: |
other/unknown material |
| وصف الملف: |
application/pdf |
| اللغة: |
Japanese |
| تدمد: |
0036-472X |
| العلاقة: |
札幌医学雑誌 = The Sapporo medical journal; 54; 625; 635; The Sapporo medical journal; https://sapmed.repo.nii.ac.jp/record/15095/files/n0036472X546625.pdfTest |
| الإتاحة: |
https://sapmed.repo.nii.ac.jp/record/15095/files/n0036472X546625.pdfTest |
| رقم الانضمام: |
edsbas.46CAB1BD |
| قاعدة البيانات: |
BASE |
