رسالة جامعية
Characterization of Violacein Producing Host Pseudoduganella sp. NI28 and Production of Deoxyviolacein with Recombinant Escherichia coli Strain
| العنوان: | Characterization of Violacein Producing Host Pseudoduganella sp. NI28 and Production of Deoxyviolacein with Recombinant Escherichia coli Strain |
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| المؤلفون: | Choi, Seong Yeol |
| المساهمون: | Mitchell, Robert J. |
| بيانات النشر: | Graduate School of UNIST |
| سنة النشر: | 2015 |
| المجموعة: | ScholarWorks@UNIST (Ulsan National Institute of Science and Technology) |
| مصطلحات موضوعية: | Violacein, Deoxyviolacein |
| الوصف: | Department of Biological Sciences ; Violacein is bacteria pigment made several strain such as J.lividum, C.violaceum, Duganella sp., Pseudoduganella sp., Collimonas sp. which found various environment from glacial to subtropical climate. The usage of violacein in the bio-application in the field is diverse to block growth of several type of living organism: gram positive bacteria, fungus, virus, protozoa, nematodes, parasites and some cancer cell line like Hep2, KM12, MOLT-4. Because of this versatile application potent, needs of amount of violacein and its derivation will be increase near future. Nevertheless this positive application prediction, the production of violacein and its derivation is just beginning stage. The crude violacein contained violacein and its derivation specific productivity is reported 12.4mg/L?h and its final concentration is 0.44g/L using C.violaceum natural violacein producing host. In recombinant strain violacein producing field, recent reported recombinant E. coli produce pure violacein 0.710g/L, however, specific productivity is 3.1mg/ L?h. On the other hands violacein measurement method is not standardized, spectrophotometry is generally used for measurement violacein but extinction coefficient doesn???t have unity every study. Moreover purity of crude violacein is also questionable without HPLC analysis. Thus violacein production field need more study for enhance productivity and standardizing measurement of crude violacein. Characterization of violacein producing strain Pseudoduganella sp. NI28 found forest soil sample. P. sp. NI28 is similar to Psedoduganella violanceinigra YIM 31327 98.8% for 16s rRNA, 95.9% for gyrB, 88.8% for vioA. Even this similarity NI28 shown better crude violacein production tendency in NB media two Pseudoduganella strains shown significantly different production of crude violacein, NI28 produced 14mg/L but type strain produced under 0.1mg at 24 hours culture. Following HPLC analysis, NI28 crude violacein contain more than 95% violacein in the extracted ... |
| نوع الوثيقة: | master thesis |
| اللغة: | unknown |
| العلاقة: | https://scholarworks.unist.ac.kr/handle/201301/16727Test; http://unist.dcollection.net/jsp/common/DcLoOrgPer.jsp?sItemId=000002071255Test |
| الإتاحة: | https://scholarworks.unist.ac.kr/handle/201301/16727Test http://unist.dcollection.net/jsp/common/DcLoOrgPer.jsp?sItemId=000002071255Test |
| رقم الانضمام: | edsbas.CCBB68CD |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |