دورية أكاديمية
Structural Systems Biology Evaluation of Metabolic Thermotolerance in Escherichia coli
| العنوان: | Structural Systems Biology Evaluation of Metabolic Thermotolerance in Escherichia coli |
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| المؤلفون: | Chang, Roger L., Andrews, Kathleen, Kim, Donghyuk, Li, Zhanwen, Godzik, Adam, Palsson, Bernhard O. |
| بيانات النشر: | AMER ASSOC ADVANCEMENT SCIENCE |
| سنة النشر: | 2018 |
| المجموعة: | ScholarWorks@UNIST (Ulsan National Institute of Science and Technology) |
| الوصف: | Genome-scale network reconstruction has enabled predictive modeling of metabolism for many systems. Traditionally, protein structural information has not been represented in such reconstructions. Expansion of a genome-scale model of Escherichia coli metabolism by including experimental and predicted protein structures enabled the analysis of protein thermostability in a network context. This analysis allowed the prediction of protein activities that limit network function at superoptimal temperatures and mechanistic interpretations of mutations found in strains adapted to heat. Predicted growth-limiting factors for thermotolerance were validated through nutrient supplementation experiments and defined metabolic sensitivities to heat stress, providing evidence that metabolic enzyme thermostability is rate-limiting at superoptimal temperatures. Inclusion of structural information expanded the content and predictive capability of genome-scale metabolic networks that enable structural systems biology of metabolism. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 0036-8075 |
| العلاقة: | SCIENCE, v.340, no.6137, pp.1220 - 1223; http://science.sciencemag.org/content/340/6137/1220Test; https://scholarworks.unist.ac.kr/handle/201301/24289Test; 3695; 30478; 2-s2.0-84878756325; 000319972800047 |
| DOI: | 10.1126/science.1234012 |
| الإتاحة: | https://doi.org/10.1126/science.1234012Test https://scholarworks.unist.ac.kr/handle/201301/24289Test http://science.sciencemag.org/content/340/6137/1220Test |
| رقم الانضمام: | edsbas.3A8EDDF0 |
| قاعدة البيانات: | BASE |
| تدمد: | 00368075 |
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| DOI: | 10.1126/science.1234012 |