التفاصيل البيبلوغرافية
| العنوان: |
Diversity, distribution, and dynamics of coral reef bacterial communities across the Hawaiian archipelago and wider Pacific Ocean |
| المؤلفون: |
Salerno, Jennifer Lee |
| بيانات النشر: |
[University of Hawaii at Manoa], [December 2013] |
| سنة النشر: |
2013 |
| المجموعة: |
ScholarSpace at University of Hawaii at Manoa |
| مصطلحات موضوعية: |
bacterial communities, Hawaiian Archipelago, Indo-Pacific Ocean, coral health |
| الوصف: |
Ph.D. University of Hawaii at Manoa 2013. ; Includes bibliographical references. ; A comprehensive investigation characterizing the diversity, distribution, and dynamics of coral-associated bacterial communities was carried out through a series of targeted observational and experimental studies. A focus was placed on four overarching research themes deemed fundamental to understanding these complex communities: method optimization, biogeography, natural variability, and environmental effects on bacterial community structure. Protocols for genomic DNA extraction and Polymerase Chain Reaction (PCR) amplification of bacterial 16S rRNA genes were optimized for high-throughput analyses of coral-associated bacterial communities associated with common Hawaiian corals. Successful gene amplification and community profiles varied with sample pre-treatment/nucleic acid extraction method combination and differed significantly between Porites lobata and Porites compressa corals. A baseline assessment of coral-associated bacteria associated with Porites lobata throughout the Hawaiian Archipelago and Indo-Pacific Ocean revealed a weak, but significant isolation by distance pattern, indicating that geographic isolation is partially responsible for driving observed divergences at the archipelagic scale. However, some geographic groupings were not distance-based, indicating that additional environmental factors may also affect the distributions of these communities. The natural variability of bacterial communities associated with colonies of Porites compressa was investigated over a five-month period at two sites around Molokaʻi, Hawaiʻi. Terminal-restriction fragment length polymorphism analysis of PCR-amplified 16S ribosomal RNA genes revealed that bacterial communities did not differ between colonies within a site but differed between sites and fluctuated from month-to-month. Analysis of environmental data revealed site differences in environmental parameters (seawater salinity, temperature, turbidity, fluorescence, ... |
| نوع الوثيقة: |
thesis |
| وصف الملف: |
application/pdf |
| اللغة: |
English |
| العلاقة: |
Theses for the degree of Doctor of Philosophy (University of Hawaii at Manoa). Zoology.; http://hdl.handle.net/10125/100747Test |
| الإتاحة: |
http://hdl.handle.net/10125/100747Test |
| رقم الانضمام: |
edsbas.D20045AC |
| قاعدة البيانات: |
BASE |
